Pollen germination, pollen–pistil interaction and crossability studies in interspecific and induced colchiploid population of Abelmoschus species

Badiger, Mahesh; Yadav, Ramesh Kumar; Sharma, Brij Mohan; Bhat, K. V.; Tomar, B S; Lata, Suman; Vinay, N.D.; Das, Anjan

doi:10.1007/s10722-023-01610-y

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…In the present investigation, interspecific crosses involving A. esculentus and wild relatives, followed by polyploidization, generated considerable variability in the amphidiploid derivatives. These results reaffirmed the earlier reports on the successful interspecific hybridization to generate considerable variability (Reddy, 2010;Arunkumar, 2015;Patel et al, 2021;Badiger et al, 2023;Singh et al, 2023b) and the existence of barriers to the transmission of gene flow from wild species to the cultivated ones (Rajamony et al, 2006;Jatkar et al, 2007;Badiger et al, 2023). Widely variable amphidiploids have also been generated from the hybrids of wheat and related species of the genera Aegilops, Secale, and Thinopyrum by Nemeth et al (2015).…”

Section: Discussionsupporting

confidence: 85%

“…Regarding the derivatives involving A. esculentus and A. moschatus variant, the seed setting ranged from 22.75 to 98.25, indicating that the seed setting was possible by using A. esculentus as a female parent. Badiger et al (2023) also confirmed that viable seeds were obtained in the direct cross. They reported that A. moschatus × A. esculentus crosses (reciprocal cross) suffered from severe pre-zygotic barriers that resulted in the formation of aborted embryos with partially filled seeds.…”

Section: Selfed Amphidiploid Derivativessupporting

confidence: 73%

“…Further, the greater vigor of the amphidiploids may be attributed to the heterozygosity of different genomes (Nasrallah et al, 2000;Chen, 2010;Fujimoto et al, 2011). Badiger et al (2023) observed that the interspecific hybrids developed between A. esculentus and wild species (A. manihot var. tetraphyllus and A. moschatus) showed intermediate morphological traits with greater alliance toward their wild parents.…”

Section: Discussionmentioning

confidence: 99%

“…Rattan and Kumar (2020) developed the protocol for embryo rescue of interspecific hybrids involving A. esculentus × A. tetraphyllus. Badiger et al (2023) elaborated the pollen germination, pollenpistil interaction, and crossability studies in interspecific and induced colchiploid populations involving crosses between A. esculentus and wild species, viz., A. manihot var. tetraphyllus and A. moschatus.…”

Section: Introductionmentioning

confidence: 99%

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Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

Suma,

Joseph John,

Bhat

et al. 2023

Front. Plant Sci.

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IntroductionThe introgression of genetic material from one species to another through wide hybridization and repeated back-crossing, plays an important role in genetic modification and enriching the cultivated gene-pool with novel genetic variations. Okra (Abelmoschus esculentus [(L.) Moench)] is a popular vegetable crop with high dietary fibre and protein, rich in essential amino acids, lysine and tryptophan. The wild Abelmoschus genepool has many desirable traits like ornamental value, short internodal length, more number of productive branches, extended bearing, perennation tendency, reduced fruit length (more consumer preferred trait), high mucilage content (medicinal value), abiotic stress tolerances such as drought, high temperature and biotic stress resistances such as okra Yellow Vein Mosaic Virus (YVMV) and Enation Leaf Curl Virus (ELCV) diseases. The repeated use of elite breeding lines led to narrowing of the genetic base of the okra crop, one of the major factors attributed to breakdown of resistance/ tolerance to biotic stresses. YVMV and ELCV are the two major diseases, causing significant yield loss in okra. Hence, wide hybridization was attempted to transfer tolerance genes from wild species to the cultivated genepool to widen the genetic base.Material and methodsThe screening of germplasm of wild Abelmoschus species at hotspots led to the identification of tolerant species (Abelmoschus pungens var. mizoramensis, A. enbeepeegeearensis, A. caillei, A. tetraphyllus and A. angulosus var. grandiflorus), which were further used in a wide-hybridization programme to generate interspecific hybrids with the cultivated okra. Presence of pre- and post-zygotic barriers to interspecific geneflow, differences in ploidy levels and genotype specific variations in chromosome numbers led to varying degrees of sterility in F1 plants of interspecific crosses. This was overcome by doubling the chromosome number of interspecific hybrids by applying Colchicine at the seedling stage. The 113 cross derivatives generated comprising amphidiploids in the F1 generation (30), F3 (14), one each in F2 and F4 generations, back cross generation in BC1F2 (03), BC1F3 (25), and BC2F3 (02), crosses between amphidiploids (27), multi-cross combinations (07) and inter-specific cross (between A. sagittifolius × A. moschatus subsp. moschatus) selfed derivatives at F8 generation (03) were characterized in the present study. Besides they were advanced through selfing and backcrossing.Results and DiscussionThe amphidiploids were found to possess many desirable genes with a considerable magnitude of linkage drag. Majority of the wide cross derivatives had an intermediate fruit morphology and dominance of wild characters viz., hispid fruits, stem, leaves, tough fruit fibre, vigorous perennial growth habit and prolonged flowering and fruiting. The fruit morphology of three BC progenies exhibited a high morphological resemblance to the cultivated okra, confirming successful transfer of useful genes to the cultivated okra genepool. The detailed morphological characteristics of the various combinations of Abelmoschus amphidiploids and the genetic enhancement of the genepool achieved in this process is reported here.

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Section: Discussionsupporting

confidence: 85%

Section: Selfed Amphidiploid Derivativessupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

Suma,

Joseph John,

Bhat

et al. 2023

Front. Plant Sci.

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“…Similarly, Pasonen et al (2001) reported a significant positive correlation between pollen-tube growth rate and seed mass in Betula pendula. Conversely in VP/MH, although the in vitro pollen germination was higher (71.7 %) (at par with SC), the fruit set (23.25 %) was significantly less as compared to VP/SC along with the lowest compatibility index (0.511) among all successful cross-combinations (Badiger et al, 2023).…”

Section: Cross-compatibility and Pollenpistil Interactionmentioning

confidence: 83%

Studies on pollen micro-morphology, pollen storage methods, and cross-compatibility among grape (Vitis spp.) genotypes

Rane,

Thakre,

Verma

et al. 2024

Front. Plant Sci.

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The knowledge of pollen morphology, suitable storage condition, and species compatibility is vital for a successful grapevine improvement programme. Ten grape genotypes from three different species, viz., Vitis vinifera L., Vitis parviflora Roxb., and Vitis champini Planc., were studied for their pollen structure and pollen storage with the objective of determining their utilization in grape rootstock improvement programs. Pollen morphology was examined through the use of a scanning electron microscope (SEM). The viability of the pollen was assessed using 2,3,5-triphenyltetrazolium chloride (TTC). In vitro pollen germination was investigated using the semi-solid medium with 10 % sucrose, 100 mg/L boric acid, and 300 mg/L calcium nitrate. The results revealed variations in pollen micro-morphology in 10 genotypes, with distinct pollen dimensions, shapes, and exine ornamentation. However, species-wise, no clear difference was found for these parameters. Pollen of V. parviflora Roxb. and Dogridge was acolporated and did not germinate. The remaining eight genotypes exhibited tricolporated pollen and showed satisfactory in vitro pollen germination. Storage temperature and duration interactions showed that, at room temperature, pollen of most of the grape genotypes can be stored for up to 1 day only with an acceptable pollen germination rate (>30 %). However, storage for up to 7 days was successfully achieved at 4 °C, except for ‘Pearl of Csaba’. The most effective storage conditions were found to be at −20 °C and −196 °C (in liquid N2), enabling pollen storage for a period of up to 30 days, and can be used for pollination to overcome the challenge of asynchronous flowering. Four interspecific combinations were studied for their compatibility, among which V. parviflora Roxb. × V. vinifera L. (Pusa Navrang) and V. parviflora Roxb. × V. champini Planc. (Salt Creek) showed high cross-compatibility, offering their potential use for grape rootstock breeding. However, V. parviflora Roxb. × V. vinifera L. (Male Hybrid) recorded the lowest compatibility index among studied crosses. In the case of self-pollinated flowers from V. parviflora Roxb. and V. parviflora Roxb. × V. champini Planc. (Dogridge), pollen failed to germinate on the stigma due to male sterility caused by acolporated pollen. As a result, the flowers of these genotypes functioned as females, which means they are ideal female parents for grape breeding without the need for the tedious process of emasculation.

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Enhancing virus resistance through pre-breeding and crossability experiments in okra (Abelmoschus esculentus (L.) Moench)

Santhiya,

Yadav,

Lata

et al. 2024

Genet Resour Crop Evol

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Pollen germination, pollen–pistil interaction and crossability studies in interspecific and induced colchiploid population of Abelmoschus species

Cited by 5 publications

References 32 publications

Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

Studies on pollen micro-morphology, pollen storage methods, and cross-compatibility among grape (Vitis spp.) genotypes

Enhancing virus resistance through pre-breeding and crossability experiments in okra (Abelmoschus esculentus (L.) Moench)

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