Pollen quality for pollinators tracks pollen quality for plants in <i>Mimulus guttatus</i>

Yeamans, Rebecca; Roulston, T’ai H.; Carr, David E.

doi:10.1890/es14-00099.1

Cited by 19 publications

(18 citation statements)

References 59 publications

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“…This is not surprising as other pollen indices, such as protein content and viability, are known to vary extensively between members of the same species, based on genotypic and environmental influences on plant health. 58,59 Using the highest level observed for pollen in this study, we can calculate that an adult honey bee might consume approximately 6 £ 10 7 copies of MIR156a per day, resulting in a maximum theoretical delivery of 6 copies per cell if 100% transfer of the diet-derived miRNA were achieved (using 1 £ 10 7 cells per bee as previously 25 ). Thus, even under these optimal theoretical conditions, it is difficult to envision dietary miRNA reaching distal tissues in biologically relevant levels unless aided by specialized mechanisms for concentrating or transferring miRNA in specific cell types.…”

Section: Discussionmentioning

confidence: 87%

Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees

et al. 2015

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The putative transfer and gene regulatory activities of diet-derived miRNAs in ingesting animals are still debated. Importantly, no study to date has fully examined the role of dietary uptake of miRNA in the honey bee, a critical pollinator in both agricultural and natural ecosystems. After controlled pollen feeding experiments in adult honey bees, we observed that midguts demonstrated robust increases in plant miRNAs after pollen ingestion. However, we found no evidence of biologically relevant delivery of these molecules to proximal or distal tissues of recipient honey bees. Our results, therefore, support the premise that pollen miRNAs ingested as part of a typical diet are not robustly transferred across barrier epithelia of adult honey bees under normal conditions. Key future questions include whether other small RNA species in honey bee diets behave similarly and whether more specialized and specific delivery mechanisms exist for more efficient transport, particularly in the context of stressed barrier epithelia.

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Section: Discussionmentioning

confidence: 87%

Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees

et al. 2015

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“…We posit that it may be due to attributes of the pollen itself or correlations with traits we did not measure. Although we can only speculate, one possibility is that more viable pollen may have higher protein content (Yeamans, Roulston, & Carr, ) which contributes to pollinator choice (Carr, Roulston, & Hart, ). Darker pollen remains more viable under heat stress (Koski & Galloway, ), and so may provide a higher‐quality reward to pollinators.…”

Section: Discussionmentioning

confidence: 99%

Pollen colour morphs take different paths to fitness

Koski

Berardi

Galloway

2020

J of Evolutionary Biology

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Colour phenotypes are often involved in communication and are thus under selection by species interactions. However, selection may also act on colour through correlated traits or alternative functions of biochemical pigments. Such forms of selection are instrumental in maintaining petal colour diversity in plants. Pollen colour also varies markedly, but the maintenance of this variation is little understood. In Campanula americana, pollen ranges from white to dark purple, with darker morphs garnering more pollinator visits and exhibiting elevated pollen performance under heat stress. Here, we generate an F2 population segregating for pollen colour and measure correlations with floral traits, pollen attributes and plant‐level traits related to fitness. We determine the pigment biochemistry of colour variants and evaluate maternal and paternal fitness of light and dark morphs by crossing within and between morphs. Pollen colour was largely uncorrelated with floral traits (petal colour, size, nectar traits) suggesting it can evolve independently. Darker pollen grains were larger and had higher anthocyanin content (cyanidin and peonidin) which may explain why they outperform light pollen under heat stress. Overall, pollen‐related fitness metrics were greater for dark pollen, and dark pollen sires generated seeds with higher germination potential. Conversely, light pollen plants produce 61% more flowers than dark, and 18% more seeds per fruit, suggesting a seed production advantage. Results indicate that light and dark morphs may achieve fitness through different means—dark morphs appear to have a pollen advantage whereas light morphs have an ovule advantage—helping to explain the maintenance of pollen colour variation.

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“…La viabilidad del polen varía sustancialmente entre y dentro de especies y puede ser reducida por distintos factores (Yeamans et al, 2014). De acuerdo con estos autores, el polen llega a ser inviable ya sea durante su desarrollo en los botones florales o después de su desarrollo completo.…”

Section: Introductionunclassified

Viabilidad y longevidad del polen en genotipos de limón mexicano estimada mediante germinación in vitro

Robles-González

Carrillo-Medrano

Cortez-Arroyo

et al. 2019

Remexca

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Los estudios de viabilidad y longevidad del polen en los genotipos seleccionados como progenitores son útiles para asegurar el éxito de las hibridaciones en un programa de mejoramiento genético. El presente estudio se llevó a cabo en el Campo Experimental Tecomán del INIFAP, en Tecomán Colima, México, durante los meses de enero y febrero de 2008 y 2009. Se estimó la viabilidad y longevidad del polen en tres variedades de limón mexicano [Citrus aurantifolia): ‘Colimex’, ‘Colimón’ y ‘Lise’, dos híbridos naturales de limón mexicano; CV 63-64 y CV 67-68 así como el ‘Citrange C-35’ (C. sinensis x Poncirus trifoliata) como testigo. Se evaluaron las formulaciones de Brewbaker y Kwack (1963); Lora et al. (2006); Leal (1969) modificado. Los tres medios de cultivo promovieron la germinación in vitro de granos de polen en proporciones muy similares, cercanas a 20%. Los genotipos, ‘Colimón’ y ‘Lise’ registraron porcentajes de germinación bajos 0.78% y 3.05% respectivamente. Por su parte, la variedad ‘Colimex’ y el hibrido natural CV 63 -64, tuvieron valores porcentuales intermedios, mientras que el ‘Citrange C-35’ y el híbrido natural CV 67-68 alcanzaron los porcentajes de viabilidad de polen más altos (43.3% y 39.69% respectivamente) y pueden funcionar como donadores de polen. El polen de todos los genotipos evaluados, conservado a temperatura ambiente, que fluctuó entre 21 °C y 30 °C, perdió casi 100% de su viabilidad a las 48 h posteriores a la antesis. Este es el primer estudio sobre viabilidad y longevidad de polen de limón mexicano usando pruebas de germinación in vitro.

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Pollen quality for pollinators tracks pollen quality for plants in Mimulus guttatus

Cited by 19 publications

References 59 publications

Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees

Negligible uptake and transfer of diet-derived pollen microRNAs in adult honey bees

Pollen colour morphs take different paths to fitness

Viabilidad y longevidad del polen en genotipos de limón mexicano estimada mediante germinación in vitro

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