Pollen size in<i>Carex</i>: The effect of different chemical treatments and mounting media

Meltsov, Vivika; Poska, Anneli; Saar, Maret

doi:10.1080/00173130802435970

Cited by 27 publications

(21 citation statements)

References 23 publications

(41 reference statements)

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“…This is in agreement with previous reports (Erdtman, 1952;Heusser, 1971;Schulze, 1978;Kosenko, 1994;Rudall et al, 2000;Sanso et al, 2005;Hofreiter, 2006). However, there are significant differences in the value of quantitative palynological characters that may to some extent be related to differences in the preparation of pollen grains (Schols et al, 2004) as well as the mounting medium (Meltsov et al, 2008 and references cited therein). We have used non-acetolysed pollen grains mounted in silicone oil for our measurements; however, all other researchers, except Sanso and Xifreda (2001), used acetolysed pollen for their observations and measurements.…”

Section: Variations In Pollen Morphological Characterssupporting

confidence: 82%

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Pollen morphology and infrageneric classification ofAlstroemeriaL. (Alstroemeriaceae)

Sarwar

Hoshino

Araki

2010

Grana

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Section: Variations In Pollen Morphological Characterssupporting

confidence: 82%

“…In most instances, acetolysing for three minutes or more does not affect the size significantly (Reitsma, 1969) or between 6% and 30% (Schols et al, 2004). Pollen grains mounted in silicon oil were also smaller than grains mounted in glycerine (Faegri & Iversen, 1975;Meltsov et al, 2008).…”

Section: Variations In Pollen Morphological Charactersmentioning

confidence: 99%

Pollen morphology and infrageneric classification ofAlstroemeriaL. (Alstroemeriaceae)

Sarwar

Hoshino

Araki

2010

Grana

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“…However, there are significant differences in the value of quantitative palynological characters that may to some extent be related to differences in the mounting media (Meltsov et al, 2008 and references therein). Pollen grains mounted in silicon oil were smaller than grains mounted in glycerine jelly (Faegri and Iversen, 1989;Meltsov et al, 2008).…”

Section: Variation In Palynological Charactersmentioning

confidence: 98%

Pollen morphology of <i>Kalmia</i> L. (Phyllodoceae, Ericaceae) and its taxonomic significance

Sarwar

Takahashi

2012

Bangladesh J. Plant Taxon

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Pollen morphology of seven taxa of Kalmia was examined using light and scanning electron microscopy (LM and SEM, respectively), or SEM alone, in search of new characters that might contribute to infrageneric classification of the genus. The Kalmia species are stenopalynous and characterized by 3-colpor(oid)ate, medium, oblate pollen united in tetrahedral tetrads with rugulate exine sculpture. However, a continuous and serial variation in all the quantitative characters and exine sculpture was revealed within the genus. Kalmia buxifolia is characterized by having the smallest pollen tetrads, largest aperture, largest 2f/D ratio and septum thicker than apocolpial exine. On the other hand, K. latifolia produces the largest pollen tetrads with smallest aperture and smallest 2f/D ratio. An evolutionary trend from rugulate to psilate has also been observed in apocolpial exine sculpture of the genus Kalmia. On the basis of acetolysed pollen characteristics, a dichotomous key for Kalmia was prepared.

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“…Concerning mounting medium, glycerol jelly tends to swell the grains about 1.25 times compared to silicone oil treatment, which does not appear to cause swelling (Moore and Webb 1978). However, a recent study performed with Carex pollen grains showed that the intraspecific variation of size was greater than the variation that could be observed due to different chemical treatments (Meltsov et al 2008).…”

Section: Influence Of Pollen Preparationmentioning

confidence: 99%

Poaceae pollen grain size as a tool to distinguish past grasslands in South America: a new methodological approach

Schüler

Behling

2010

Veget Hist Archaeobot

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Despite the dominance of grasslands during the last glacial period, especially in South America, the highly uniform morphology of Poaceae pollen grains has so far allowed only very few palynological studies based on Poaceae pollen. In our study we compare two methods of distinguishing between South American grassland ecosystems based on quantitative morphology of Poaceae pollen grains. We investigated data sets from Páramo in southern Ecuador, Campos de Altitude and Campos in south-eastern and southern Brazil as well as data sets from the Pampa in Argentina by measuring the pollen grain length, grain width, pore diameter and annulus width. Firstly we investigated the potential influence of chemical treatment of pollen grains on pollen grain size as well as the measurement setting for defining the boundary conditions for using Poaceae pollen grains in a palaeoecological investigation. Finally the measured pollen grain parameters were analyzed by comparison of average grain length using statistical tests. This approach reveals highly significant differences in average grain size between all grassland ecosystems. Assuming that a certain grain size range can be assigned to a certain Poaceae taxon, conclusions about differences and similarities in taxa composition can be derived. We used two methods of multivariate data analysis. One uses the pollen grain parameters directly for a Principle Component Analysis (PCA). The other is an already established method in grassland ecology which defines parameter based pollen grain types to investigate similarities between grassland ecosystems. Both approaches confirm the results of the grain length analysis. In this work we demonstrate that the method we developed has the potential to provide acquisition of so far inaccessible information on spatial and temporal patterns and dynamics of South American grasslands.

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Pollen size inCarex: The effect of different chemical treatments and mounting media

Cited by 27 publications

References 23 publications

Pollen morphology and infrageneric classification ofAlstroemeriaL. (Alstroemeriaceae)

Pollen morphology and infrageneric classification ofAlstroemeriaL. (Alstroemeriaceae)

Pollen morphology of <i>Kalmia</i> L. (Phyllodoceae, Ericaceae) and its taxonomic significance

Poaceae pollen grain size as a tool to distinguish past grasslands in South America: a new methodological approach

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