Pollen-specific pectin methylesterase involved in pollen tube growth

Guo-wei, Tian; Chen, Min-Huei; Zaltsman, Adi; Citovsky, Vitaly

doi:10.1016/j.ydbio.2006.02.026

Cited by 192 publications

(177 citation statements)

References 53 publications

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“…Cellulose, hemicellulose, and pectin are three major carbohydrates making up the primary cell wall in most plant cells, while the wall of the pollen tube tip is only composed of a single layer of pectin. Thus, pectin methylesterases (PMEs) likely play a central role in pollen tube growth (Tian et al, 2006). Our RGA down-regulated list includes a group of genes encoding putative PMEs, such as VANGUARD1 (VGD1; At2g47040) and VGDH1 (At2g47030), both of which are represented by the same probe set identifier in the Affymetrix array, and VGDH2 (At3g62170).…”

Section: Rga Predominantly Regulates Stamen and Pollen Developmentmentioning

confidence: 99%

“…Two other PME genes, AtPPME1 (At1g69940) and At5g07410, represented by the same probe set identifier in the Affymetrix array, are also down-regulated by RGA. AtPPME1 plays a role in determining the shape of the pollen tube and the rate of its elongation (Tian et al, 2006). Identification of a group of RGA down-regulated PME genes in unpollinated floral buds indicates that GA is required to relieve RGA repression of these genes during pollen development so that the pollen tube can elongate normally after pollination.…”

Section: Rga Predominantly Regulates Stamen and Pollen Developmentmentioning

confidence: 99%

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Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Hou

Shen

et al. 2008

Plant Physiology

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Gibberellin (GA) plays important roles in regulating many aspects of plant development. GA derepresses its signaling pathway by promoting the degradation of DELLA proteins, a family of nuclear growth repressors. Although the floral organ identity is established in flowers of the GA-deficient mutant ga1-3, the growth of all floral organs is severely retarded. In particular, abortive anther development in ga1-3 results in male sterility. Genetic analysis has revealed that various combinations of null mutants of DELLA proteins could gradually rescue floral organ defects in ga1-3 and that RGA is the most important DELLA protein involved in floral organ development. To elucidate the early molecular events controlled by RGA during flower development, we performed whole-genome microarray analysis to identify genes in response to the steroid-inducible activation of RGA in ga1-3 rgl2 rga 35S:RGA-GR. Although DELLA proteins were suggested as transcriptional repressors, similar numbers of genes were down-regulated or up-regulated by RGA during floral organ development. More than one-third of RGA down-regulated genes were specifically or predominantly expressed in stamens. A significant number of RGA-regulated genes are involved in phytohormone signaling or stress response. Further expression analysis through activation of RGA by steroid induction combined with cycloheximide identified eight genes as immediate targets of RGA. In situ hybridization and transgenic studies further showed that the expression pattern and function of several selected genes were consistent with the predictions from microarray analysis. These results suggest that DELLA regulation of floral organ development is modulated by multiple phytohormones and stress signaling pathways.

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Section: Rga Predominantly Regulates Stamen and Pollen Developmentmentioning

confidence: 99%

Section: Rga Predominantly Regulates Stamen and Pollen Developmentmentioning

confidence: 99%

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Hou

Shen

et al. 2008

Plant Physiology

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“…PMEs are classified in two distinct groups depending on the presence/absence of an N-terminal extension domain (the PRO region) showing similarity with the pectin methylesterase inhibitor (PMEI) domain (Micheli, 2001). PMEs from group 1 do not have a PMEI domain but can be inhibited by PMEIs (Röckel et al, 2008), whereas PMEs from group 2 can contain from one to three PMEI domains (Tian et al, 2006). It is hypothesized that the PRO region is cleaved during maturation of the PME, as so far, only PMEs lacking this domain were found in the cell wall (Micheli, 2001).…”

mentioning

confidence: 99%

“…The first direct evidence for the crucial roles of PMEs during pollen tube growth was described in two knockout mutants, vanguard1 (vgd1; Jiang et al, 2005) and Atppme1 (Tian et al, 2006). VGD1 (At2g47040) encodes a pollen-specific group 2 PME.…”

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confidence: 99%

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

et al. 2014

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Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling, and biochemical analyses was used on wild-type and Atpme48 mutant plants. We showed that AtPME48 is specifically expressed in the male gametophyte and is the second most expressed PME in dry and imbibed pollen grains. Pollen grains from homozygous mutant lines displayed a significant delay in imbibition and germination in vitro and in vivo. Moreover, numerous pollen grains showed two tips emerging instead of one in the wild type. Immunolabeling and Fourier transform infrared analyses showed that the degree of methylesterification of the homogalacturonan was higher in pme482/2 pollen grains. In contrast, the PME activity was lower in pme482/2, partly due to a reduction of PME48 activity revealed by zymogram. Interestingly, the wild-type phenotype was restored in pme482/2 with the optimum germination medium supplemented with 2.5 mM calcium chloride, suggesting that in the wild-type pollen, the weakly methylesterified homogalacturonan is a source of Ca 2+ necessary for pollen germination. Although pollen-specific PMEs are traditionally associated with pollen tube elongation, this study provides strong evidence that PME48 impacts the mechanical properties of the intine wall during maturation of the pollen grain, which, in turn, influences pollen grain germination.

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“…This suggests that loss of function of RMD may affect the trafficking of pectin and callose. Pectin distribution is required for the formation of pollen tubes with a normal appearance, and the disruption of a single pectin methylesterase frequently causes severe pollen tube defects (Tian et al, 2006;Rounds and Bezanilla, 2013), suggesting that the altered deposition of pectin in the pollen tube wall of rmd mutants also causes abnormal pollen tube growth.…”

Section: Rmd Controls Cytoplasmic Streaming and Cell Wall Componentsmentioning

confidence: 99%

Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth

Yang

Zhang

et al. 2018

Plant Physiol.

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Pollen-specific pectin methylesterase involved in pollen tube growth

Cited by 192 publications

References 53 publications

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth

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