Pollen wall pattern in Arabidopsis

Xu, Te; Zhang, Cheng; Zhou, Qing; Yang, Zhong‐Nan

doi:10.1007/s11434-016-1062-6

Cited by 32 publications

(30 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SAPs contain proteins derived from male germ cells, associated with sporopollenin reception. A primary pattern is defined by the plasma membrane undulation and primexine reception sites at this stage (Xu et al ., ). We found that pro‐tectum and pro‐baculum layers were generated with increasing deposition and polymerization of sporopollenin, but no difference was found in np1‐4 at the tetrad stage, indicating that primexine formation and initial accumulation of sporopollenin were not affected in the mutant.…”

Section: Discussionmentioning

confidence: 97%

Rice No Pollen 1 (NP1) is required for anther cuticle formation and pollen exine patterning

et al. 2017

View full text Add to dashboard Cite

Angiosperm male reproductive organs (anthers and pollen grains) have complex and interesting morphological features, but mechanisms that underlie their patterning are poorly understood. Here we report the isolation and characterization of a male sterile mutant of No Pollen 1 (NP1) in rice (Oryza sativa). The np1-4 mutant exhibited smaller anthers with a smooth cuticle surface, abnormal Ubisch bodies, and aborted pollen grains covered with irregular exine. Wild-type exine has two continuous layers; but np1-4 exine showed a discontinuous structure with large granules of varying size. Chemical analysis revealed reduction in most of the cutin monomers in np1-4 anthers, and less cuticular wax. Map-based cloning suggested that NP1 encodes a putative glucose-methanol-choline oxidoreductase; and expression analyses found NP1 preferentially expressed in the tapetal layer from stage 8 to stage 10 of anther development. Additionally, the expression of several genes involved in biosynthesis and in the transport of lipid monomers of sporopollenin and cutin was decreased in np1-4 mutant anthers. Taken together, these observations suggest that NP1 is required for anther cuticle formation, and for patterning of Ubisch bodies and the exine. We propose that products of NP1 are likely important metabolites in the development of Ubisch bodies and pollen exine, necessary for polymerization, assembly, or both.

show abstract

Section: Discussionmentioning

confidence: 97%

Rice No Pollen 1 (NP1) is required for anther cuticle formation and pollen exine patterning

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This result suggests that the enzyme activity of OsACOS12 might be lower than that of ACOS5 in Arabidopsis or that there may be some functional divergence for fatty acid metabolism. The pollen wall patterning for a specific plant species was a conserved and elaborate process [41]. The slightly different substrates and products derived from OsACOS12 probably led to the defective pollen surface in the transgenic line.…”

Section: Discussionmentioning

confidence: 99%

OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice

Guo

et al. 2016

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

BackgroundSporopollenin is a major component of the pollen exine pattern. In Arabidopsis, acyl-CoA synthetase5 (ACOS5) is involved in sporopollenin precursor biosynthesis. In this study, we identified its orthologue, OsACOS12, in rice (Oryza sativa) and compared the functional conservation of ACOS in rice to Arabidopsis.ResultsSequence analysis showed that OsACOS12 shares 63.9 % amino acid sequence identity with ACOS5. The osacos12 mutation caused by a pre-mature stop codon in LOC_Os04g24530 exhibits defective sexine resulting in a male sterile phenotype in rice. In situ hybridization shows that OsACOS12 is expressed in tapetal cells and microspores at the transcript level. The localization of OsACOS12-GFP demonstrated that OsACOS12 protein is accumulated in tapetal cells and anther locules. OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis.Conclusions OsACOS12 is an orthologue of ACOS5 that is essential for sporopollenin synthesis in rice. ACOS5 and OsACOS12 are conserved for pollen wall formation in monocot and dicot species.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0943-9) contains supplementary material, which is available to authorized users.

show abstract

“…7 The pollen wall, which is mainly made up of exine and intine, is highly stable and prevents the digestion and decomposition of pollen. 8 In particular, extine consisting of sporopollenin provides physical strength and chemical resistance, thereby preserving the contents of the pollen. 9 It is therefore necessary to employ a wall-disruption technique to promote nutrient release and the pollen's biological effects so that the utilization of bee pollen in the food industry can be expanded.…”

Section: Introductionmentioning

confidence: 99%

Effect of ultrasonic and ball‐milling treatment on cell wall, nutrients, and antioxidant capacity of rose (Rosa rugosa) bee pollen, and identification of bioactive components

et al. 2019

View full text Add to dashboard Cite

BACKGROUND: Bee pollen has been regarded as a complete nutritional human dietary supplement but its nutrient absorption and biological effects may be restricted by the complex pollen wall. The aim of this study was to explore the effects of ultrasonic and ball-milling treatment on the release of nutritional components and on in vitro and in vivo antioxidant effects of rose (Rosa rugosa) bee pollen. RESULTS: Bee pollen walls were broken to varying degrees, nutrients were released, and in vitro and in vivo antioxidant effects of bee pollen were improved. The scavenging effects of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2 ′ -azinobis(3-ethylbenzothiazolone-6-sulfonic acid) (ABTS) radicals, and oxygen radical absorbance capacity (ORAC) were improved. In aging mice, wall-breaking treatment led to better organ recovery, enhanced superoxide dismutase (SOD) and catalase (CAT) effects, and malondialdehyde (MDA) reduction. Eight compounds of rose bee pollen ethanol extract, including isorhamnetin 3-O-diglucoside and N ′ , N ′′ , N ′′′ -dicaffeoyl p-coumaroyl spermidine were identified by ultra-performance liquid chromatography electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-QTOF-MS/MS) assay.CONCLUSION: This study showed that ultrasonic treatment had greater wall-disruption effects of bee pollen on nutrient release and antioxidant effect promotion. In conclusion, rose bee pollen, with wall-breaking treatments, may have potential value as an ingredient in functional food processing.

show abstract

Pollen wall pattern in Arabidopsis

Cited by 32 publications

References 45 publications

Rice No Pollen 1 (NP1) is required for anther cuticle formation and pollen exine patterning

Rice No Pollen 1 (NP1) is required for anther cuticle formation and pollen exine patterning

OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice

Effect of ultrasonic and ball‐milling treatment on cell wall, nutrients, and antioxidant capacity of rose (Rosa rugosa) bee pollen, and identification of bioactive components

Contact Info

Product

Resources

About