Pollution profile of waterborne bacterial and fungal community in urban Rivers of Pearl River estuary: Microbial safety assessment

Abstract

Waterborne pathogens in urban rivers of estuarine areas pose a great threat to human health. In this study, both bacterial and fungal community structures of four urban rivers in the Pearl River estuarine area were investigated using high-throughput sequencing. Results showed that the diversity and richness indices of the bacterial community were higher than that of fungi. Taxonomic analysis indicated that the most abundant bacterial phylum was Actinobacteria, while the most abundant fungal phylum was Ascomycota. In addition, 35 major pathogens were found at the level of genus with Mycobacterium being the most abundant. Specifically, pathogens of Burkholderiales, Bartonella, Rhizobiales, Bordetella and Brucella were first detected in this area, although with relative low abundances. Spearman correlation analysis revealed that temperature, TOC and TDP were significant factors shaping both total microbial community and pathogenic microbial community structure. TDP was positively correlated with almost all the potential pathogenic genera. PICRUSt analysis further predicted that bacterial infectious disease was the most abundant pathway associated with microbial functions of human disease in all the studied rivers. This work provides useful information not only for in-depth understanding of bacterial and fungal communities in urban rivers of estuarine areas under anthropogenic disturbances but also for environmental risk management of potential waterborne pathogens.

The Community Structure of eDNA in the Los Angeles River Reveals an Altered Nitrogen Cycle at Impervious Sites

“…Matsuoka et al found similar results along a river network in Japan in 2019, where they found that fungal DNA assemblages had a spatial structure and samples that were closer to one another tended to be more similar. Overall, our results agree with the numerous studies of urban, eutrophic, and brackish freshwater bodies since proteobacteria, bacteroidetes, firmicutes, cyanobacteria, chloroflexi, actinobacteria, and acidobacteria were all well-represented [77][78][79][80]. The elevated presence of Verrucomicrobia and Gammaproteobacteria aligned more with the brackish metagenome [80].…”

Partitional Clustering and Differential Abundance Analysis Reveal the Community Structure of eDNA in the Los Angeles River

“…Matsuoka et al found similar results along a river network in Japan in 2019, where they found that fungal DNA assemblages had a spatial structure and samples which were closer to one another tended to be more similar. Overall, our results agree with numerous studies of urban, eutrophic, and brackish freshwater bodies since Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, Chloroflexi, Actinobacteria, Acidobacteria were all well-represented [66,67,68,69]. The elevated presence of Verrucomicrobia and Gammaproteobacteria aligned more with the brackish metagenome [69].…”

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