Poloxam Thermosensitive Hydrogels Loaded with hFGF2-Linked Camelina Lipid Droplets Accelerate Skin Regeneration in Deep Second-Degree Burns

Abstract

Burn injuries are difficult to manage due to the defect of large skin tissues, leading to major disability or even death. Human fibroblast growth factor 2 (hFGF2) is known to promote burn wound healing. However, direct administration of hFGF2 to the wound area would affect the bioactivity. To provide a supportive environment for hFGF2 and control its release in a steady fashion, in this research, we developed novel thermosensitive poloxam hydrogels delivered with hFGF2-linked Camelina lipid droplets (CLD-hFGF2 hydrogels). Cryopreserved scanning electron microscopy (SEM) results indicated that the incorporation of CLD-hFGF2 does not significantly affect the inner structure of hydrogels. The rheological properties showed that CLD-hFGF2 hydrogels gelated in response to temperature, thus optimizing the delivery method. In vitro, CLD-hFGF2 could be released from hydrogels for 3 days after drug delivery (the release rate was 72%), and the release solution could still promote the proliferation and migration of NIH3T3 cells. In vivo, compared with hydrogels alone or with direct CLD-hFGF2 administration, CLD-hFGF2 hydrogels had the most obvious effect on deep second-degree burn wound healing. This work indicates that CLD-hFGF2 hydrogels have potential application value in burn wound healing.

Polyacrylamide Hydrogel Containing Calendula Extract as a Wound Healing Bandage: In Vivo Test

“…The bandage made from the formulation also showed a reduction in the amount of exudate as well as macrophage proliferation. Afrin et al [ 57 ] and Zhang et al [ 58 ] also confirmed the efficiency of hydrogels in the wound healing process in an in vivo study. The anti-inflammatory action observed in the results was attributed to flavonols (specifically rutin) present in the calendula extract.…”

Research progress related to thermosensitive hydrogel dressings in wound healing: a review

“…Zhang et al encapsulated camelina lipid droplet (CLD)-hFGF2 in thermosensitive hydrogels. 190 The poloxamer composite hydrogels were synthesized from P188 and P407. The shell of these spherical micelles consists of a polyoxyethylene nucleus and an expanded polyoxyethylene chain.…”