Poly(3-hydroxybutyrate) Production by Isolated<i>Halomonas</i>sp. KM-1 Using Waste Glycerol

Kawata, Yoshikazu; Aiba, Sei‐ichi

doi:10.1271/bbb.90459

Cited by 88 publications

(48 citation statements)

References 10 publications

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“…Among these, the lowest Mn PHAs was derived from Halomonas sp. KM-1 cultivated in a medium supplemented with 3% of waste glycerol at 29 9 10 3 g mol -1 [20]. Some researchers have published data that we will compare with our observation.…”

Section: Gel Permeation Chromatographysupporting

confidence: 69%

“…Differing from the other carbon sources that have been studied for PHAs biosynthesis, both pure and crude glycerol used as carbon sources for PHAs production were reported with significantly low Mw PHAs because of glycerol esterification of PHAs resulting in chain termination (end-capping) [5,45]. It has been reported that the PHAs produced from biodieselbased glycerol showed Mw ranging from 786 9 10 3 to 87 9 10 3 g mol -1 and Mn ranging from 215 9 10 3 to 29 9 10 3 g mol -1 for different types of microorganisms and culture conditions [4,5,8,20,21,23]. Among these, the lowest Mn PHAs was derived from Halomonas sp.…”

Section: Gel Permeation Chromatographymentioning

confidence: 99%

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Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

Teeka

Imai

Reungsang

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Biodiesel-contaminated wastewater was used to screen for PHAs-producing bacteria by using crude glycerol as the sole carbon source. A gram-negative THA_AIK7 isolate was chosen as a potential PHAs producer. The 16S rRNA phylogeny indicated that THA_AIK7 isolate is a member of Novosphingobium genus which is supported by a bootstrap percentage of 100% with Novosphingobium capsulatum. The 1,487 bp of 16S rRNA gene sequence of THA_AIK7 isolate has been deposited in the GenBank database under the accession number HM031593. Polymer content of 45% cell dry weight was achieved in 72 h with maximum product yield coefficient of 0.29 g PHAs g⁻¹ glycerol. Transmission electron micrograph results exhibited the PHAs granules accumulated inside the bacterial cell. PHAs polymer production in mineral salt media supplemented with 2% (w/v) of crude glycerol at initial pH 7 was extracted by the sodium hypochlorite method. Polymer film spectrographs from Nuclear magnetic resonance displayed a pattern of signal virtually identical to spectra of commercial PHB. Thermal analysis by Differential scanning calorimeter showed a melting temperature at 179°C. Molecular weight analysis by Gel permeation chromatography showed two main peaks of 133,000 and 700 g mol⁻¹ with weight-average molecular weight value of 23,800 and number-average molecular weight value of 755. Endotoxinfree of PHAs polymer was preliminarily assessed by a negative result of the gel-clot formation, Pyrotell® Single test vial, at sensitivity of 0.25 EU ml⁻¹. To our knowledge, this is the first reported test of endotoxin-free PHAs naturally produced from gram-negative bacteria which could be used for biomedical application.

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Section: Gel Permeation Chromatographysupporting

confidence: 69%

Section: Gel Permeation Chromatographymentioning

confidence: 99%

Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

Teeka

Imai

Reungsang

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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“…Under such conditions, glycerol is generally converted into P(3HB) by various native PHA producing bacteria. 5,[83][84][85][86][87] As shown in Figure 3, glycerol is converted to glyceraldehyde-3-phosphate (GAP) by three enzymes, glycerol kinase (GlpK), glycerol-3-phosphate dehydrogenase (GlpD), and triosephosphate isomerase (TPI). GAP is an intermediate in the glycolysis pathway and eventually metabolized to pyruvate.…”

Section: Production and Characterization Of Polyhydrox-yalkanoates Frmentioning

confidence: 99%

Bioplastics from waste glycerol derived from biodiesel industry

Zhu

Chiu

Nakas

et al. 2013

J of Applied Polymer Sci

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Polyhydroxyalkanoates (PHAs) are polyesters that can be biologically synthesized by many microorganisms and engineered plants that have been investigated by microbiologists, biochemists, polymer scientists, material engineers, and medical researchers for several decades. Research on microbial production of PHAs has been extensively focused on using pure carbon sources, such as sugars and fatty acids. Practical considerations of production costs of PHAs have resulted in research efforts to use alternative renewable and inexpensive feedstocks. One potential feedstock for the production of PHA polymers is the glycerol waste byproduct of biodiesel production. The major focus of this review is the production of PHA polymers from glycerol. A review of biosynthetic pathways for PHAs production from glycerol, current production of waste glycerol in biodiesel industry, physical and mechanical properties of PHAs, and applications of PHAs in the areas of packaging industry, implant materials, drug carrier, biofuels, are covered.

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“…KM-1 (Kawata and Aiba, 2010), osmophilic organism (Koller et al, 2005), Pseudomonas oleovorans NRRL B-14682 and Pseudomonas corrugata 388 (Ashby et al, 2004) from different sources of biodiesel feedstock. Among these, in small scale cultivation, H. hydrothermalis could accumulate PHB content up to 75.8% of the cell dry weight (CDW) with 3 g/L PHB concentration by using mineral salt medium (MSM) supplemented with 2% (w/v) of 95% glycerol content from Jatropha biodiesel byproduct (Shrivastav et al, 2010) In Thailand, as the biodiesel industry grows, research study for biodiesel-derived waste glycerol conversion into useful product should be done in order to minimize the environmental problem caused by this waste stream.…”

Section: Introductionmentioning

confidence: 99%

Screening of PHA-Producing Bacteria Using Biodiesel-Derived Waste Glycerol as a Sole Carbon Source

Teeka

Imai

Cheng

et al. 2010

J. of Wat. & Envir. Tech.

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Different sources of wastewater and soil were used to screen for PHA-producing bacteria using biodiesel-derived waste glycerol as a sole carbon source by the Nile red staining method together with polymer determination. Twelve out of twenty-six isolates from biodiesel-contaminated wastewater consortium were screened for their PHA accumulation ability by cultivation in mineral salt medium supplemented with waste glycerol. The AIK7 isolate was chosen as a potential PHA producer. The PHA production on waste glycerol was examined using pure glycerol as a control substrate. The PHA content of AIK7 isolate cultivated in 10 g/L glycerol could reach 35% cell dry weight in 72 hours from waste glycerol and 33% cell dry weight in 120 hours from pure glycerol cultivation. It can be seen that at this content of waste glycerol, AIK7 isolate is effectively capable of biotransforming glycerol into polymer from low-grade glycerol.

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Poly(3-hydroxybutyrate) Production by IsolatedHalomonassp. KM-1 Using Waste Glycerol

Cited by 88 publications

References 10 publications

Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

Bioplastics from waste glycerol derived from biodiesel industry

Screening of PHA-Producing Bacteria Using Biodiesel-Derived Waste Glycerol as a Sole Carbon Source

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