Poly(3-hydroxybutyrate) production by Rhodopseudomonas sp. S16-VOGS3 cells grown in digested sludge

Touloupakis, Eleftherios; Chatziathanasiou, Angeliki; Ghanotakis, Demetrios F.; Carlozzi, Pietro; Pecorini, Isabella

doi:10.1016/j.eti.2023.103058

Cited by 8 publications

(4 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For PHB determination, a total of 30 mL of the biomass was centrifuged at 14,000 rpm for 8 min, and the supernatant was removed. PHB was determined in the form of crotonic acid by HPLC [35]. Three mL of the solid phase was diluted to 30 mL with distilled water, and 5.0 mL of this was destined for acid digestion to crotonic acid by boiling it in 1.0 mL of pure sulfuric acid for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Influence of Aeration Rate on Uncoupled Fed Mixed Microbial Cultures for Polyhydroxybutyrate Production

Castagnoli,

Falcioni,

Touloupakis

et al. 2024

Sustainability

Self Cite

View full text Add to dashboard Cite

The use of residual streams as feedstock for the production of polyhydroxyalkanoates (PHAs) is growing steadily, as it allows the valorization of waste and nutrients otherwise disposed of and the potential production of a biodegradable bioplastic. To date, the environmental and economic costs associated with this process limit its scale-up, which is why it is important to identify possible solutions and optimize the costliest steps. With this in mind, a laboratory-scale sequenced batch reactor (SBR, 5 L) was constructed to allow the selection of a mixed microbial culture able to convert volatile fatty acids (VFAs) into PHA. The reactor is fed with synthetic water containing VFAs, ammonium, phosphate, and micronutrients, typical compounds of fermented streams of certain wastes, such as cheese whey, food waste, or wastewater sludge. The biomass selected and produced by this first reactor is sent to an accumulation reactor, which is fed with a solution rich in VFAs, allowing the accumulation of PHAs. The role of aeration and its impacts on the main process parameters were analyzed. Three scenarios corresponding to different aeration rates were analyzed: 0.08, 0.16, and 0.32 vvm. The SBR was operated at an organic load rate of 600 mgCOD L−1d−1, under a dynamic feeding regime (feast–famine) and a short hydraulic retention time (HRT; 1 day). The results obtained showed that a value of 0.32 enabled better selection and better settling of the sludge. Furthermore, a potential correlation between aeration rate and VFA and NH4+ consumption rates was identified. The resulting biomass was able to accumulate up to 0.15 ± 0.02 g PHAgVSS−1.

show abstract

Section: Methodsmentioning

confidence: 99%

Influence of Aeration Rate on Uncoupled Fed Mixed Microbial Cultures for Polyhydroxybutyrate Production

Castagnoli,

Falcioni,

Touloupakis

et al. 2024

Sustainability

Self Cite

View full text Add to dashboard Cite

show abstract

“…PNSB is used as a nutrient-providing source for rice via N fixation processes [14], [15]. aerobic conditions [13], [41]. In particular, R. palustris TLS12, VNS19, VNS32, VNS62, and VNW95 strains have increased content of available NH4 + in acid sulfate soil [21].…”

Section: Selecting N-fixing Pnsb That Can Live In Saline Soilmentioning

confidence: 99%

“…Moreover, microbiological approaches are also promising, while purple nonsulfur bacteria (PNSB) are one of the most potent approaches, because PNSB can diversely habituate, such as photoautotroph, chemoautotroph, and chemoheterotroph [12]  [13]. Some species of PNSB belong to the genera Rhodopseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrogen-Fixing Purple Nonsulfur Bacteria Originating from Acid Saline Soils of a Rice-Shrimp Farm

Anh,

Xuan,

Xuan

et al. 2024

Indonesian J. Agric. Res

View full text Add to dashboard Cite

The study was conducted to (i) isolate, select, and identify strains of purple nonsulfur bacteria (PNSB), which can fix nitrogen (N), from soil and water in a rice-shrimp integrated system, (ii) to determine the capacity of the selected potent PNSB strains in producing plant growth promoting substances. The isolation resulted in 57 pure PNSB strains from 36 soil samples and 36 water samples of rice-shrimp paddy fields in Thanh Phu - Ben Tre. Among them, 49 strains survived under pH 5.0 conditions, 24 of which grew well under microaerobic light (ML) and aerobic dark (AD) conditions in a basic isolation medium (BIM) containing NaCl 5‰. Two strains (S01 and S06) with the greatest N fixation were identified by 16S rRNA techniques as Rhodobacter sphaeroides. Their N production was 16.9 mg L-1 under the ML condition and 32.1 mg L-1 under the AD condition. Moreover, two R. sphaeroides S01 and S06 strains performed P solubilization at 0.382-2.954 mg L-1 from Al-P, 3.81-4.28 mg L-1 from Fe-P, and 3.87-4.74 mg L-1 from Ca-P, and production of plant growth promoting substances, such as IAA (12.3-15.5 mg L-1), EPS (1.09-1.58 mg L-1), siderophores (10.7-53.6%) and ALA (1.68-2.82 mg L-1) under both the incubating conditions.

show abstract

Biomanufacturing of value-added chemicals from lignin

Liu,

Ellis,

Mhatre

et al. 2024

Current Opinion in Biotechnology

View full text Add to dashboard Cite

Poly(3-hydroxybutyrate) production by Rhodopseudomonas sp. S16-VOGS3 cells grown in digested sludge

Cited by 8 publications

References 54 publications

Influence of Aeration Rate on Uncoupled Fed Mixed Microbial Cultures for Polyhydroxybutyrate Production

Influence of Aeration Rate on Uncoupled Fed Mixed Microbial Cultures for Polyhydroxybutyrate Production

Nitrogen-Fixing Purple Nonsulfur Bacteria Originating from Acid Saline Soils of a Rice-Shrimp Farm

Biomanufacturing of value-added chemicals from lignin

Contact Info

Product

Resources

About