Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Dariš, Barbara; Knez, Željko

doi:10.2478/acph-2020-0007

Cited by 19 publications

(10 citation statements)

References 60 publications

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“…Furthermore, PHB has piezoelectric properties which can be considered as an essential job to invigorate bone development and recovery 11,12 . The nanocomposites of PHB and bioactive inorganic clays such as nano‐hydroxyapatite (n‐HA), nanosized calcium phosphates (CaP), nano‐bioglass, and Herafill have been found to give strong arm and bioactivity to the composites as investigated elsewhere 13 . Micro‐scale or nano‐scale inorganic phases conventionally utilized for the preparation of polymer nanocomposites which were mainly synthetic materials.…”

Section: Introductionmentioning

confidence: 97%

Fabrication and properties of polyhydroxybutyrate/kaolin nanocomposites and evaluation of their biocompatibility for biomedical applications

Ankush

Pugazhenthi

Vasanth

2021

J of Applied Polymer Sci

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The polyhydroxybutyrate biopolymer nanocomposites (C1–C10) were fabricated by solvent casting method with different loading of kaolin and polyethylene glycol. Scanning electron microscopy showed that the microstructure of the composites varied with different kaolin loading. X‐ray diffraction and Fourier transform infrared spectroscopy analysis confirm the presence of kaolin in the polymer matrix due to the intercalation and formation of hydrogen bond. The contact angle of the nanocomposites decreased with increasing kaolin loading indicating an improvement in wettability of the nanocomposites. Thermogravimetric and differential scanning calorimetry analysis showed that the Tmax and Tm of the nanocomposites increased with increasing kaolin loading. The mechanical property of the nanocomposite fabricated with 10 wt% kaolin (C10) was found to have identical mechanical property with natural bone that was selected as an optimum nanocomposite. The nanocomposite showed prolonged blood clotting time exhibiting anticoagulant nature of the nanocomposite. Moreover, low protein adsorption (168 ± 8 μg/cm2), suppressed platelet adhesion (75 ± 2 × 109 platelets/cm2) and less complement activation (118 ± 5 mg/dl for C3 and 658 ± 5 mg/dl for C4) showed the improvement in surface properties of the nanocomposite. In vitro bioactivity studies revealed the formation of hydroxyapatite layer on the surface of the nanocomposites. Eventually, the nanocomposites (C10) showed no cytotoxic effect on MG‐63 cells as tested through MTT assay and it is biologically safe.

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Section: Introductionmentioning

confidence: 97%

Fabrication and properties of polyhydroxybutyrate/kaolin nanocomposites and evaluation of their biocompatibility for biomedical applications

Ankush

Pugazhenthi

Vasanth

2021

J of Applied Polymer Sci

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“…These polymers serve as reserves of carbon and energy to allow cell survival during starved conditions (Jendrossek and Handrick 2002 ). They have attracted industrial interest because they are completely biodegradable, non-toxic, biocompatible and naturally produced (Anderson and Dawes 1990 ; Dariš and Knez 2020 ). Paracoccus denitrificans , a gram-negative bacterium, accumulates poly(3-hydroxybutyrate) (PHB) as insoluble granules inside the cell (Ueda et al 1992 ).…”

Section: Introductionmentioning

confidence: 99%

Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans

2021

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Poly(3-hydroxybutyrate) (PHB) granule formation in Paracoccus denitrificans Pd1222 was investigated by laser scanning confocal microscopy (LSCM) and gas chromatography analysis. Cells that had been starved for 2 days were free of PHB granules but resynthesized them within 30 min of growth in fresh medium with succinate. In most cases, the granules were distributed randomly, although in some cases they appeared in a more organized pattern. The rates of growth and PHB accumulation were analyzed within the frame of a Genome-Scale Metabolic Model (GSMM) containing 781 metabolic genes, 1403 reactions and 1503 metabolites. The model was used to obtain quantitative predictions of biomass yields and PHB synthesis during aerobic growth on succinate as sole carbon and energy sources. The results revealed an initial fast stage of PHB accumulation, during which all of the acetyl-CoA originating from succinate was diverted to PHB production. The next stage was characterized by a tenfold lower PHB production rate and the simultaneous onset of exponential growth, during which acetyl-CoA was predominantly drained into the TCA cycle. Previous research has shown that PHB accumulation correlates with cytosolic acetyl-CoA concentration. It has also been shown that PHB accumulation is not transcriptionally regulated. Our results are consistent with the mentioned findings and suggest that, in absence of cell growth, most of the cellular acetyl-CoA is channeled to PHB synthesis, while during exponential growth, it is drained to the TCA cycle, causing a reduction of the cytosolic acetyl-CoA pool and a concomitant decrease of the synthesis of acetoacetyl-CoA (the precursor of PHB synthesis).

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“…These polymers serve as reserves of carbon and energy to allow cell survival during starved conditions (Jendrossek and Handrick 2002). They have attracted industrial interest because they are completely biodegradable, non-toxic, biocompatible and naturally produced (Anderson and Dawes 1990;Dariš and Knez 2020). Paracoccus denitri cans, a gram-negative bacterium, accumulates poly(3hydroxybutyrate) (PHB) as insoluble granules inside the cell (Ueda et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans

Bordel

Spanning

Santos-Beneit

2021

Preprint

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Poly(3-hydroxybutyrate) (PHB) granule formation in Paracoccus denitrificans Pd1222 was investigated by laser scanning confocal microscopy (LSCM) and gas chromatography analysis. Cells that had been starved for 2 days were free of PHB granules but resynthesized them within 30 minutes of growth in fresh medium with succinate. In most cases, the granules were distributed randomly, although in some cases they appeared in a more organized pattern. The rates of growth and PHB accumulation were analyzed within the frame of a Genome-Scale Metabolic Model (GSMM) containing 781 metabolic genes, 1403 reactions and 1503 metabolites. The model was used to obtain quantitative predictions of biomass yields and PHB synthesis during aerobic growth on succinate as sole carbon and energy sources. The results revealed an initial fast stage of PHB accumulation, during which all of the acetyl-CoA originating from succinate was diverted to PHB production. The next stage was characterized by a tenfold lower PHB production rate and the simultaneous onset of exponential growth, during which acetyl-CoA was predominantly drained into the TCA cycle. Previous research has shown that PHB accumulation correlates with cytosolic acetyl-CoA concentration. It has also been shown that PHB accumulation is not transcriptionally regulated. Our results are consistent with the mentioned findings and suggest that, in absence of cell growth, most of the cellular acetyl-CoA is channeled to PHB synthesis, while during exponential growth, it is drained to the TCA cycle, causing a reduction of the cytosolic acetyl-CoA pool and a concomitant decrease of the synthesis of acetoacetyl-CoA (the precursor of PHB synthesis).

show abstract

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Cited by 19 publications

References 60 publications

Fabrication and properties of polyhydroxybutyrate/kaolin nanocomposites and evaluation of their biocompatibility for biomedical applications

Fabrication and properties of polyhydroxybutyrate/kaolin nanocomposites and evaluation of their biocompatibility for biomedical applications

Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans

Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans

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