Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering

Abstract

In this study, fibrous scaffolds based on poly(γ-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N -hydroxyethyl-l-glutamine units; and second, modification with the short peptide sequence azidopentanoyl-GGGRGDSGGGY-NH , using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10 -10 pm/cm ). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm and 200 ± 100 fm/cm RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs. Copyright © 2015 John Wiley & Sons, Ltd.

RGDS- and SIKVAVS-Modified Superporous Poly(2-hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications

“…Moreover, tyrosine and acetylcysteine were incorporated into the peptide's C‐ and N‐terminus, respectively, to enable both radioiodination and immobilization of the peptide via a Michael type reaction with the activated double bond (Figure ). Let us note that the effective cell adhesion on the RGD‐modified surfaces was observed in concentrations ranging 1–1000 fmol cm −2 , i.e., at spatial distance between ligands 440 and 9 nm . The peptide concentration and spatial distance were crucial also for the MSC spreading and differentiation .…”

Colloidally stable polypeptide‐based nanogel: Study of enzyme‐mediated nanogelation in inverse miniemulsion

“…The starting polymer, poly(γ‐benzyl‐L‐glutamate) (PBLG) (I), was prepared according to our previous work (for more details, see Supporting Information) …”

“…The starting polymer, poly(γ-benzyl-L-glutamate) (PBLG) (I), was prepared according to our previous work (for more details, see Supporting Information). 28 The poly[N 5 -(2-hydroxypropyl)-L-glutamine-ran-N 5 -propargyl-L-glutamine-ran-N 5 -(6-aminohexyl)-L-glutamine-ran-N 5 -(2-(4hydroxyphenyl)ethyl)-L-glutamine] (P2HPG) polymer precursor was prepared through the modification of glutamate side chains, as follows (Scheme 1). First, using HBr, PBLG (I) was partially debenzylated to poly(γ-benzyl-L-glutamate-co-glutamic acid) (PBLG-co-PGA) (II).…”

Controlled drug release for tissue engineering

“…Growth factors 58 , synthetic chemicals 59 , and compounds such as graphene 60 have also been used to modify the surface of a scaffold matrix. Various peptides 61–63 , often short amino acid sequences of ECM proteins, are also used to increase biocompatibility of scaffold surfaces. Other approaches such as argon plasma 64 , phosphonate groups 65 , or cold atmospheric plasma 66 modifications have also improved adhesion and cellular infiltration and proliferation on scaffolds.…”