Poly-cis-isoprene Degradation by Nocardia sp. BSTN01 Isolated from Industrial Waste

Sarkar, Biraj; Gupta, Aayatti Mallick; Shah, Maulin P.; Mandal, Sukhendu

doi:10.1007/s12010-022-03854-3

Cited by 6 publications

(4 citation statements)

References 45 publications

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“…It was also found that poly-cis-isoprene was degraded by Nocardia sp. after six weeks by 55.3% [42].…”

Section: Confirmation Of Nr Degradation By E Coli Ay1 and A Oryzae 32...mentioning

confidence: 93%

“…No available studies shed light on degraded rubber's antioxidant and antibacterial activity. Still, Sarkar et al [42] found an increase in the total protein inside Nocardia BSTN01 cells up to 623.6 µg mL −1 following NR degradation.…”

Section: Biological Application Of Biodegraded Nrmentioning

confidence: 95%

“…Our results also agreed with the study of Maheswaran et al [45], who used SEM to measure the variations in carbonyl index on polyethylene pterephthalate surface by bacterial consortium. In addition, Sarkar et al [42] revealed adhesive growth of Nocardia sp. BSTN01 on the surface of natural and synthetic rubber using SEM.…”

Section: Sem Imaging Of Degraded Nrmentioning

confidence: 99%

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Eco-Friendly Degradation of Natural Rubber Powder Waste Using Some Microorganisms with Focus on Antioxidant and Antibacterial Activities of Biodegraded Rubber

et al. 2023

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Natural rubber (NR) powder wastes contribute to the pollution of the environment and pose a risk to human health. Therefore, Escherichia coli AY1 and Aspergillus oryzae were used to degrade NR in the present investigation. The biodegradation was further confirmed using E. coli AY1 and A. oryzae’s ability to create biofilm, which grew on the surface of the NR. Additionally, the biodegraded NR was examined by scanning electron microscopy (SEM), attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopy, and gas chromatography–mass spectrometry (GC–MS). The highest weight loss (69%) of NR was detected (p < 0.05) after 210 d of incubation with the mixed microbial culture (E. coli AY1 + A. oryzae). In the SEM, the surface of the control treatment appeared uniform and normal, whereas the surface of the microbial treatment displayed an irregular shape, with apparent particle deformation and surface erosion. After biodegradation by E. coli AY1 and A. oryzae, the particle size range of the untreated NR dropped from (5.367–9.623 µm) to (2.55–6.549 µm). After treating NR with E. coli AY1 and A. oryzae, new bands appeared in the ATR–FTIR technique; others shifted down in the range of 3910–450 cm−1, suggesting the existence of active groups belonging to alcohol, secondary amine, aromatic amine, conjugated anhydride, aldehyde, alkene, and halo compounds. On the other hand, the GC–MS profile reports a significant decline (p < 0.05) in the amount of hydrocarbons while simultaneously reporting a significant increase (p < 0.05) in the proportion of oxygenated, sulfurous, and nitrogenous compounds. These active groups are attributed to the antioxidant and antibacterial properties of biodegraded NR by a mixture of E. coli AY1 and A. oryzae, which rose 9-fold (p < 0.05) compared to untreated NR. Through the use of this research, we will be able to transform NR waste into a valuable product that possesses both antioxidant and antibacterial properties.

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“…It was also found that poly-cis-isoprene was degraded by Nocardia sp. after six weeks by 55.3% [42].…”

Section: Confirmation Of Nr Degradation By E Coli Ay1 and A Oryzae 32...mentioning

confidence: 93%

Section: Biological Application Of Biodegraded Nrmentioning

confidence: 95%

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Eco-Friendly Degradation of Natural Rubber Powder Waste Using Some Microorganisms with Focus on Antioxidant and Antibacterial Activities of Biodegraded Rubber

et al. 2023

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“…In the majority of recent investigations, it has been observed that the biodegradation process of NR has shown a rather slow rate, despite the successful identification and isolation of a limited number of functional bacteria capable of degrading this material ( Bosco et al, 2018 ; Joseph et al, 2022; Sarkar et al, 2022 ; Cheng et al, 2023 ). The duration of microbial culture has shown a range of 30–120 days, but assessment methods such as weight loss or SEM have only identified little deterioration.…”

Section: Prospects For Rubber Degradation In the Futurementioning

confidence: 99%

Assembly strategies for rubber-degrading microbial consortia based on omics tools

Cui,

Jiang,

Zhang

et al. 2023

Front. Bioeng. Biotechnol.

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Numerous microorganisms, including bacteria and fungus, have been identified as capable of degrading rubber. Rubber biodegradation is still understudied due to its high stability and the lack of well-defined pathways and efficient enzymes involved in microorganism metabolism. However, rubber products manufacture and usage cause substantial environmental issues, and present physical-chemical methods involve dangerous chemical solvents, massive energy, and trash with health hazards. Eco-friendly solutions are required in this context, and biotechnological rubber treatment offers considerable promise. The structural and functional enzymes involved in poly (cis-1,4-isoprene) rubber and their cleavage mechanisms have been extensively studied. Similarly, novel bacterial strains capable of degrading polymers have been investigated. In contrast, relatively few studies have been conducted to establish natural rubber (NR) degrading bacterial consortia based on metagenomics, considering process optimization, cost effective approaches and larger scale experiments seeking practical and realistic applications. In light of the obstacles encountered during the constructing NR-degrading consortia, this study proposes the utilization of multi-omics tools to discern the underlying mechanisms and metabolites of rubber degradation, as well as associated enzymes and effective synthesized microbial consortia. In addition, the utilization of omics tool-based methods is suggested as a primary research direction for the development of synthesized microbial consortia in the future.

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Biodegradation of vulcanized natural rubber by enriched bacterial consortia

Cheng,

Wei,

et al. 2024

Chemical Engineering Journal

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Poly-cis-isoprene Degradation by Nocardia sp. BSTN01 Isolated from Industrial Waste

Cited by 6 publications

References 45 publications

Eco-Friendly Degradation of Natural Rubber Powder Waste Using Some Microorganisms with Focus on Antioxidant and Antibacterial Activities of Biodegraded Rubber

Eco-Friendly Degradation of Natural Rubber Powder Waste Using Some Microorganisms with Focus on Antioxidant and Antibacterial Activities of Biodegraded Rubber

Assembly strategies for rubber-degrading microbial consortia based on omics tools

Biodegradation of vulcanized natural rubber by enriched bacterial consortia

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