Poly(dimethylsiloxane) Microchip Electrophoresis with Contactless Conductivity Detection for Measurement of Chemical Warfare Agent Degradation Products

Ding, Yongsheng; Garcı́a, Carlos D.; Rogers, Kim R.

doi:10.1080/00032710701792943

Cited by 32 publications

(25 citation statements)

References 45 publications

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“…A negative [44][45][46][47] or positive [48][49][50][51] polarity mode was applied; polarity in this context refers to the polarity on the detection side. Some researchers added an electroosmotic flow modifier to the electrolyte to suppress or reverse the EOF [33,34,[49][50][51], while others used no EOF modification [44][45][46][47]. The negative polarity mode offers some advantages over the positive, as the inorganic cations and anions are effectively separated from the NA degradation products.…”

Section: Introductionmentioning

confidence: 99%

In situ determination of nerve agents in various matrices by portable capillary electropherograph with contactless conductivity detection

Kubáň

Seiman

Makarõtševa

et al. 2011

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

In situ determination of nerve agents in various matrices by portable capillary electropherograph with contactless conductivity detection

Kubáň

Seiman

Makarõtševa

et al. 2011

Journal of Chromatography A

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“…The combination of amperometric detection with ME provides a powerful approach to the determination of a variety of biologically important compounds including reactive oxygen species (ROS) [7], reactive nitrogen species (RNS) [8], catecholamines [9, 10], thiols [11, 12], and carbohydrates [13-15]. This technique has been used in a variety of applications including biomedical [16], agricultural [17], forensics [18, 19], and environmental analyses [20]. …”

Section: Introductionmentioning

confidence: 99%

In‐channel amperometric detection for microchip electrophoresis using a wireless isolated potentiostat

2011

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The combination of microchip electrophoresis (ME) with amperometric detection leads to a number of analytical challenges that are associated with isolating the detector from the high voltages used for the separation. While methods such as end-channel alignment and the use of decouplers have been employed, they have limitations. A less common method has been to utilize an electrically isolated potentiostat. This approach allows placement of the working electrode directly in the separation channel without using a decoupler. This paper explores the use of microchip electrophoresis and electrochemical detection (ME-EC) with an electrically isolated potentiostat for the separation and in-channel detection of several biologically important anions. The separation employed negative polarity voltages and tetradecyltrimethylammonium bromide (TTAB, as a buffer modifier) for the separation of nitrite (NO2-), glutathione (GSH), ascorbic acid (AA), and tyrosine (Tyr). A half-wave potential (E½) shift of approximately negative 500 mV was observed for NO2- and H2O2 standards in the in-channel configuration compared to end channel. Higher separation efficiencies were observed for both NO2- and H2O2 with the in-channel detection configuration. The limits of detection were approximately two-fold lower and the sensitivity was approximately two-fold higher for in-channel detection of nitrite when compared to end-channel. The application of this microfluidic device for the separation and detection of biomarkers related to oxidative stress is described.

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“…Collaborations with other faculty members have enabled the application of microchips to the analysis of chemical warfare agents [15], the development of the first lab-on-a-robot (Integrated Microchip – Capillary Electrophoresis, Power Supply, Electrochemical Detector, Wireless Unit, and Mobile Platform) [16] and the multivariate evaluation of the separation conditions for five bisphenols [17]. …”

Section: Capillary Electrophoresis Microchip-ce and Electrochemicalmentioning

confidence: 99%

Research Spotlight: The Next Big Thing Is Actually Small

Garcı́a

2012

Bioanalysis

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Summary Recent developments in materials, surface modifications, separation schemes, detection systems, and associated instrumentation have allowed significant advances in the performance of lab-on-a-chip devices. These devices, also referred to as micro total analysis systems (µTAS), offer great versatility, high throughput, short analysis time, low cost, and more importantly, performance that is comparable to standard bench-top instrumentation. To date, µTAS have demonstrated advantages in a significant number of fields including biochemical, pharmaceutical, military, and environmental. Perhaps most importantly, µTAS represent excellent platforms to introduce students to microfabrication and nanotechnology, bridging chemistry with other fields such as engineering and biology, enabling the integration of various skills and curricular concepts. Considering the advantages of the technology and the potential impact to society, our research program aims to address the need for simpler, more affordable, faster, and portable devices to measure biologically-active compounds. Specifically, the program is focused on the development and characterization of a series of novel strategies towards the realization of integrated microanalytical devices. One key aspect of our research projects is that the developed analytical strategies must be compatible with each other, therefore enabling their use in integrated devices. The program combines spectroscopy, surface chemistry, capillary electrophoresis, electrochemical detection, and nanomaterials. This article discusses some of the most recent results obtained in two main areas of emphasis: Capillary Electrophoresis, Microchip-CE, Electrochemical Detection, andInteraction of Proteins with Nanomaterials

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Poly(dimethylsiloxane) Microchip Electrophoresis with Contactless Conductivity Detection for Measurement of Chemical Warfare Agent Degradation Products

Cited by 32 publications

References 45 publications

In situ determination of nerve agents in various matrices by portable capillary electropherograph with contactless conductivity detection

In situ determination of nerve agents in various matrices by portable capillary electropherograph with contactless conductivity detection

In‐channel amperometric detection for microchip electrophoresis using a wireless isolated potentiostat

Research Spotlight: The Next Big Thing Is Actually Small

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