2000
DOI: 10.1002/1438-5171(200006)1:2<99::aid-simo99>3.0.co;2-k
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Poly(Ethylene Glycol): An Ideal Spacer for Molecular Recognition Force Microscopy/Spectroscopy.

Abstract: In this review, we report recent molecular recognition studies of our group. The surface chemistry by which ligands are covalently coupled to force microscopy tips is described. Poly(ethylene glycol), which is used as spacer for the ligands, was shown to be a most suitable crosslinker for force spectroscopy and microscopy experiments. Basic principles of force spectroscopy are discussed and the successful application of this technique to several biological systems is demonstrated. Information about kinetic rat… Show more

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Cited by 88 publications
(59 citation statements)
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“…2, bottom (labeled A. anophagefferens), is a histogram of rupture forces between a probe functionalized with the MAb to the target alga immobilized by using the Ab method described above. The histogram for the f-d rupture force taken at 12 nN/s load rate exhibits a well-defined peak corresponding to a force of 246 11 pN, which is within the expected range for single Ab-Ag bonds [28]. For these reasons, we believe that the data in Fig.…”
Section: A Algal Immobilizationsupporting
confidence: 64%
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“…2, bottom (labeled A. anophagefferens), is a histogram of rupture forces between a probe functionalized with the MAb to the target alga immobilized by using the Ab method described above. The histogram for the f-d rupture force taken at 12 nN/s load rate exhibits a well-defined peak corresponding to a force of 246 11 pN, which is within the expected range for single Ab-Ag bonds [28]. For these reasons, we believe that the data in Fig.…”
Section: A Algal Immobilizationsupporting
confidence: 64%
“…Finally, the tips were treated in an MAb [18] solution that was specific for surface antigens of A. anophagefferens (8.5 g/mL of MAb in 100 mM phosphate buffer, pH 7.2) for 1 h, rinsed with phosphate buffer, and then stored in phosphate buffer at 4 C until use. Tips could typically be stored for several weeks after they had been functionalized [28].…”
Section: A Tip Functionalization With Mabmentioning
confidence: 99%
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“…Several other interactions, such as Van der Waals, electrostatic or hydrophobic forces can contribute to the attractive interaction between the tip and the substrate. Long polymeric linkers, in particular polyethylene glycol (PEG) [24], are usually intercalated between the tip surface and one of the moieties in order to separate this unspecific contribution from the studied interaction [25]. Thus, the typically short-ranged unspecific interactions are broken before any stress is exerted on the complex (Figure 2).…”
Section: Double-tethered Dfsmentioning
confidence: 99%