Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties

Abstract

Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting.

Targeted Delivery of Interleukin-12 Plasmid into HepG2 cells through Folic Acid Conjugated Graphene Oxide Nanocarrier

“…The ndings from our studies revealed that transfection e ciency with G0-PEI polyplexes was remarkably higher than simple PEI at all tested N/P ratios. Additionally, a folic acid ligand (GO-PEI-FA) has been added to the polymeric nanoparticle to enhance delivery to the target site, which has been broadly used in the treatment of cancers overexpressing folate receptors, such as liver, lung, breast, and ovarian [23,36,37].…”

Contactless Resolution of Inflammatory Signals in Tailored Macrophage-Based Cell Therapeutics

“…34,35 We selected branched PEI because of its additional advantages over linear PEI, providing primary, secondary, and tertiary amino groups that more effectively bind nucleic acids, whereas low-molecular-weight PEI lowers the cytotoxic potential of the system. 36 Furthermore, the modification of SPION with PEI molecules increases the nucleic acid transfection efficiency of PEI alone, as magnetic-based systems can be internalized in cells under the guidance of an EMF via magnetofection.…”

Efficient Chlorostannate Modification of Magnetite Nanoparticles for Their Biofunctionalization

“…Altogether, these facts suggest better binding of the mAb-FA antibody with MNP-SnCl(DMF)-mAb-FA, possibly due to the lower hydrolysis rate of SnCl2 in DMF. It should be noted that the developed magnetic IC metrology could be useful for the investigation of many other functionalized magnetic nanoparticles, which are extensively studied, for example, for targeted hyperthermia treatments of cancer [44], the isolation and identification of circulating tumor cells [45], the diagnosis and treatment of cardiovascular diseases [46], and magnetic systems for personalized and precision medicine [47], etc. In addition, studies like those discussed above on the interaction of FAconju- It should be noted that the developed magnetic IC metrology could be useful for the investigation of many other functionalized magnetic nanoparticles, which are extensively studied, for example, for targeted hyperthermia treatments of cancer [44], the isolation and identification of circulating tumor cells [45], the diagnosis and treatment of cardiovascular diseases [46], and magnetic systems for personalized and precision medicine [47], etc.…”