Poly(Ethylene oxide) Hydrogels: Valuable Candidates for an Artificial Pancreas?

Alexandre, Eliane; Boudjema, K.; Schmitt, Bertrand; Cinqualbre, J; Jaeck, D.; Lutz, P.

doi:10.1021/bk-2006-0924.ch009

Cited by 2 publications

(2 citation statements)

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“…The cell entrapment occurs in the polymer solution and there are no small monomers which are typically toxic to the cells. Furthermore, PEO hydrogels have been approved by the US Food and Drug Administration for biomedical application and their biocompatible properties have already led to a number of biomedical applications (Alexandre et al 2003;Doycheva et al 2004). Therefore, future investigations should focus on additional optimization of the gel properties relevant for cell immobilization, shape and size of immobilized gels, and finding the right ratio of entrapped cells and support material.…”

Section: Operational Stability Of Immobilized Candida Boidinii Cells mentioning

confidence: 99%

Hydrogels based on u.v.-crosslinked poly(ethylene oxide) – matrices for immobilization of Candida boidinii cells for xylitol production

Winkelhausen

Jovanovic-Malinovska

Kuzmanova

et al. 2008

World J Microbiol Biotechnol

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Hydrogels based on high molecular weight poly(ethylene oxide) were synthesized by u.v.-irradiation of aqueous solutions in presence of the photoinitiator, (4-benzoylbenzyl)trimethylammonium chloride and different crosslinkers, poly(ethylene glycol), diacrylates and N,N 0 -methylenebisacrylamide. Candida boidinii cells were immobilized in these hydrogels and the gels were characterized in regards to gel fraction yield, degree of equilibrium swelling, shear storage and loss moduli. In addition, the number average molecular weight between crosslinks and the mesh size were estimated. The incorporated yeast cells considerably affected the viscoelastic properties of the gels. Immobilized C. boidinii cells were used for conversion of xylose to xylitol. Of the immobilized systems tested, only the system with poly(ethylene oxide) crosslinked with N,N 0 -methylenebisacrylamide exhibited xylitol production. The operational stability of this system was evaluated by seven repeated-batch runs performed in Erlenmeyer flasks in duration of 55 days. The progressive improvement of xylose consumption, up to 73.5%, stopped in the fifth cycle, after which it dropped to 42.7%. Although xylitol concentration never reached more than 4.2 g l -1 , xylitol was produced in each of the seven cycles. The cell leakage of 1.8 g l -1 during the first 45 days, indicated very good stability of the system.

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Section: Operational Stability Of Immobilized Candida Boidinii Cells mentioning

confidence: 99%

Hydrogels based on u.v.-crosslinked poly(ethylene oxide) – matrices for immobilization of Candida boidinii cells for xylitol production

Winkelhausen

Jovanovic-Malinovska

Kuzmanova

et al. 2008

World J Microbiol Biotechnol

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“…Biomimetic hydrogels mimic biological extracellular matrix structures and absorb large amount of water without disintegrating (1). Notable examples of biomimetic hydrogels include: poly(acrylic acid), poly(ethylene oxide), poly(Nvinylcaprolactam), collagen, alginate and silk fibroin (1)(2)(3)(4)(5)(6)(7). In particular, hydrogels of poly(N-vinylcaprolactam) (PVCL) and silk fibroin have been extensively studied in the field of biomedical and tissue engineering (8,9).…”

Section: Introductionmentioning

confidence: 99%

Nanostructure Evolution of Biomimetic Hydrogel from Silk Fibroin and Poly(N-Vinylcaprolactam): A Small Angle Neutron Scattering Study

Balu¹,

Whittaker²,

Mata³

et al. 2018

ACS Symposium Series

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The nanostructure of biomimetic hydrogels fabricated using regenerated silk fibroin (RSF) protein and thermoresponsive poly(N-vinylcaprolactam) (PVCL) polymer via physical and chemical crosslinking methods have been studied using Small Angle Neutron Scattering (SANS) technique.RSF showed no gelation and/or secondary structure (Gaussian coil) change at temperatures between 12 °C and 35 °C, whereas PVCL formed physical hydrogels by molecular self assembly exhibiting swollen coil to Gaussian coil structure change above a lower critical solution temperature (LCST) of ~32 °C. RSF-PVCL blend solutions were also observed to form physical hydrogels above this LCST.However, RSF-PVCL hybrid system exhibited swollen coil to collapsed coil structure change, suggesting relatively increased gelation compared to PVCL. Contrast variation SANS experiments demonstrate that in RSF-PVCL hybrid system, RSF provokes the intrinsic structural changes in PVCL without undergoing any structural change of its own. Unlike PVCL and other biomimetic polymer hydrogels, RSF hydrogels exhibited ordered structures containing hydrophobic domains which exhibit sharp interfaces with hydrophilic domains and/or amorphous protein matrix. Further, photochemically crosslinked RSF and RSF-PVCL hybrid hydrogels showed variation in the order of secondary structures in hydrophilic domains, which can be associated to their reported difference in water uptake behavior.

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Poly(Ethylene oxide) Hydrogels: Valuable Candidates for an Artificial Pancreas?

Cited by 2 publications

References 0 publications

Hydrogels based on u.v.-crosslinked poly(ethylene oxide) – matrices for immobilization of Candida boidinii cells for xylitol production

Hydrogels based on u.v.-crosslinked poly(ethylene oxide) – matrices for immobilization of Candida boidinii cells for xylitol production

Nanostructure Evolution of Biomimetic Hydrogel from Silk Fibroin and Poly(N-Vinylcaprolactam): A Small Angle Neutron Scattering Study

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