Poly(glycerol sebacate) combined with chondroitinase ABC promotes spinal cord repair in rats

Abstract

This study was to investigate the feasibility of PGS combined with ChABC for repairing the transection of spinal cords (TSC) in rats. A thoracic 10 (T10) TSC model of rats was employed. The effects of PGS with ChABC on the morphology and histological structure of the spinal cords, Basso, Beattie, Bresnahan (BBB) scores, and the expression of GAP-43 and NF-200 were comparatively studied. The BBB scores indicated that all rats with TSC were paralyzed immediately after surgery and then recovered hind limb movement gradually, but did not fully recover until the end of week 12. The rats treated with PGS alone, ChABC alone, and PGS/ChABC recovered significantly (p < 0.05) better than the control rats with TSC only. The PGS/ChABC treated rats recovered significantly (p < 0.05) more movement function than the rats treated with PGS or ChABC treated alone. The spinal cords in the control rats showed lusterless surfaces, big holes, and big scars; in both PGS rats and ChABC rats showed lucent surfaces, small holes, and small scars; in PGS/ChABC rats showed the best. The expression of GAP-43 and NF-200 in the TSC region was hardly detected in the control rats, moderately detected in PGS or ChABC rats, and highly detected in PGS/ChABC rats. In conclusion, both PGS and ChABC alone could promote nerve regeneration and partially recover the movement function in TSC rats. A combination of PGS and ChABC resulted in augmented nerve regeneration and functional recovery. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1770-1777, 2018.

Poly(glycerol-co-diacids) Polyesters: From Glycerol Biorefinery to Sustainable Engineering Applications, A Review

“…Poly­(glycerol sebacate) (PGSeb) modified by grafting of poly­(methyl methacrylate) has been proposed as candidates for nerve tissue engineering Electrospun nanofibers of PMMA–PGSeb in blends with gelatin supported differentiation of PC12 cells onto neuron like phenotypes. Unmodified PGSeb has also been shown a promising material on nerve regeneration in vivo . On animal models, PGSeb was implanted onto spinal cord defects along a promoter of neuron development (ChABC), and the nerve regeneration and functional recovery was evaluated.…”

Poly(Glycerol Sebacate) in Biomedical Applications—A Review of the Recent Literature

“…A simple, porous PGS film proved to promote motor function recovery through nerve generation by enhancing axon growth and neuron sprouting in a rat model, where the complete transection of the spinal cord was employed. [ 189 ] These results were even improved if combined with chondroitinase ABC. For nerve TE, electrically conductive grafts have shown great potential.…”

“…However, this technique is constrained due to a restricted supply of donated nerves, the necessity for a second surgery, donor site morbidity, loss of function, a mismatch between donor nerve and recipient site and clinically functional recovery rates of only 80%. [187][188][189] Research within a variety of biological or artificial nerve grafts has been carried out to supplement or even substitute autologous nerve grafts. Nerve grafts have to fulfill several requirements as biocompatibility, biodegradability, permeability, biochemical properties, as well as macro and microarchitecture.…”

Polyglycerol Hyperbranched Polyesters: Synthesis, Properties and Pharmaceutical and Biomedical Applications

“…The device combined three stimulating factors, a micro-patterned surface, a neurotrophic gradient membrane and seeding with Schwann cells, and it was demonstrated that neural stem cells could attach, proliferate, and differentiate successfully on this device. PGSeb was also evaluated for the repair of the transection of spinal cords [163].…”