Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells

Li, Yonggang; Siripanyaphinyo, Uamporn; Tumkosit, Uranan; Noranate, Nitchakarn; A-nuegoonpipat, Atchareeya; Pan, Yang; Kameoka, Masanori; Kurosu, Takeshi; Ikuta, Kazuyoshi; Takeda, Naokazu; Anantapreecha, Surapee

doi:10.1186/1743-422x-9-114

Cited by 60 publications

(46 citation statements)

References 54 publications

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“…They have frequently been used as a cellular model to study the role of bronchial epithelial cells in airway inflammation [17], and can induce IFN-β in response to poly(I:C) [18]. To reassess the kinetics of well-characterized IFN-β gene expression, we first performed a time course analysis of IFN-β mRNA accumulation in BEAS-2B cells following 2 hours of treatment with poly(I:C).…”

Section: Resultsmentioning

confidence: 99%

Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine

Abe

Ishigami

Shyu

et al. 2012

Biochemical and Biophysical Research Communications

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Interferon-beta (IFN-β) is a critical antiviral cytokine and is essential for innate and acquired immune responses to pathogens. Treatment with polyinosinic:polycytidylic acid (poly(I:C)) induces transient accumulation of IFN-β mRNA, which involves an increase and a decrease of IFN-β mRNA. This phenomenon has been extensively analyzed as a model for understanding the mechanisms of gene induction in response to external stimuli. Using a new RNA metabolic labeling method with ethynyluridine to directly measure de novo RNA synthesis and RNA stability, we reassessed both de novo synthesis and degradation of IFN-β mRNA. We found that transcriptional activity is maintained after the maximum accumulation of IFN-β mRNA following poly(I:C) treatment on immortalized human bronchial epithelial cells. We also observed an unexpected change in the stability of IFN-β mRNA before and after the maximum accumulation. The results indicate that this method of RNA metabolic labeling provides a general approach for the simultaneous analysis of transcriptional activity and mRNA stability coupled with transcriptional timing.

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Section: Resultsmentioning

confidence: 99%

Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine

Abe

Ishigami

Shyu

et al. 2012

Biochemical and Biophysical Research Communications

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“…Previous research demonstrated that Poly I:C can reduce the replication of Chikungunya virus in vitro [39] and pretreatment of mice with poly I:C provided significant protection against SARS and influenza infection [40]. Poly I:C was also effective as an adjuvant for Foot and Mouth disease vaccine in pigs [23,41] and for influenza virus vaccine studies in chickens [42,43].…”

Section: Discussionmentioning

confidence: 99%

Poly I:C adjuvanted inactivated swine influenza vaccine induces heterologous protective immunity in pigs

Thomas

Wang

Sreenivasan

et al. 2015

Vaccine

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Swine influenza is widely prevalent in swine herds in North America and Europe causing enormous economic losses and a public health threat. Pigs can be infected by both avian and mammalian influenza viruses and are sources of generation of reassortant influenza viruses capable of causing pandemics in humans. Current commercial vaccines provide satisfactory immunity against homologous viruses; however, protection against heterologous viruses is not adequate. In this study, we evaluated the protective efficacy of an intranasal Poly I:C adjuvanted UV inactivated bivalent swine influenza vaccine consisting of Swine/OH/24366/07 H1N1 and Swine/CO/99 H3N2, referred as PAV, in maternal antibody positive pigs against an antigenic variant and a heterologous swine influenza virus challenge. Groups of three-week-old commercial-grade pigs were immunized intranasally with PAV or a commercial vaccine (CV) twice at 2 weeks intervals. Three weeks after the second immunization, pigs were challenged with the antigenic variant Swine/MN/08 H1N1 (MN08) and the heterologous Swine/NC/10 H1N2 (NC10) influenza virus. Antibodies in serum and respiratory tract, lung lesions, virus shedding in nasal secretions and virus load in lungs were assessed. Intranasal administration of PAV induced challenge viruses specific-hemagglutination inhibition- and IgG antibodies in the serum and IgA and IgG antibodies in the respiratory tract. Importantly, intranasal administration of PAV provided protection against the antigenic variant MN08 and the heterologous NC10 swine influenza viruses as evidenced by significant reductions in lung virus load, gross lung lesions and significantly reduced shedding of challenge viruses in nasal secretions. These results indicate that Poly I:C or its homologues may be effective as vaccine adjuvants capable of generating cross-protective immunity against antigenic variants/heterologous swine influenza viruses in pigs.

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“…20) is a synthetic double-stranded RNA (dsRNA) analog. 93 In according with Li et al, 94 the CHIKV was found to be sensitive to the innate immune response induced by (215). 77,92 Moreover, activation of the TRL3 leads to an innate immune response against many different types of viruses.…”

Section: Polyinosinic Acidmentioning

confidence: 90%

The medicinal chemistry of Chikungunya virus

Silva-Júnior

Leoncini

Rodrigues

et al. 2017

Bioorganic & Medicinal Chemistry

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Arthropod-borne viruses (arboviruses) are an important threat to human and animal health globally. Among these, zoonotic diseases account for billions of cases of human illness and millions of deaths every year, representing an increasing public health problem. Chikungunya virus belongs to the genus Alphavirus of the family Togariridae, and is transmitted mainly by the bite of female mosquitoes of the Aedes aegypti and/or A. albopictus species. The focus of this review will be on the medicinal chemistry of Chikungunya virus, including synthetic and natural products, as well as rationally designed compounds.

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Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells

Cited by 60 publications

References 54 publications

Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine

Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine

Poly I:C adjuvanted inactivated swine influenza vaccine induces heterologous protective immunity in pigs

The medicinal chemistry of Chikungunya virus

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