Poly(L-co-D,L-lactic acid-co-trimethylene carbonate) for extrusion-based 3D printing: Comprehensive characterization and cytocompatibility assessment

Pedrini, Flavia; Gomes, Rodrigo César; Moraes, Ariana Souza; Antunes, Bianca Sabino Leocádio; Motta, Adriana Cristina; Dávila, José Luis; Hausen, Moema Alencar; Komatsu, Daniel; Duek, Eliana Aparecida Rezende

doi:10.1016/j.polymer.2023.126585

Cited by 3 publications

(1 citation statement)

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“…Furthermore, investigations by Wang et al 15 underscore that attributes such as mechanical and biodegradable characteristics, porous structure, as well as the facilitation of chondrocyte stimulation and specificity, represent essential strategies for biomaterials in this area. Consequently, the investigation of the manipulation properties of the PLDLA-TMC terpolymer assumes significant importance, given its nuanced characteristics for tissue engineering 13 and its compatibility with the 3D printing process, 16 standing out as a promising new material for the regeneration of cartilage tissue.…”

Section: Introductionmentioning

confidence: 99%

Meniscal repair with additive manufacture of bioresorbable polymer: From physicochemical characterization to implantation of 3D printed poly (L-co-D, L lactide-co-trimethylene carbonate) with autologous stem cells in rabbits

Komatsu,

Cabrera,

Quevedo

et al. 2024

J Biomater Appl

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Three-dimensional (3D) structures are actually the state-of-the-art technique to create porous scaffolds for tissue engineering. Since regeneration in cartilage tissue is limited due to intrinsic cellular properties this study aims to develop and characterize three-dimensional porous scaffolds of poly (L-co-D, L lactide-co-trimethylene carbonate), PLDLA-TMC, obtained by 3D fiber deposition technique. The PLDLA-TMC terpolymer scaffolds (70:30), were obtained and characterized by scanning electron microscopy, gel permeation chromatography, differential scanning calorimetry, thermal gravimetric analysis, compression mechanical testing and study on in vitro degradation, which showed its amorphous characteristics, cylindrical geometry, and interconnected pores. The in vitro degradation study showed significant loss of mechanical properties compatible with a decrease in molar mass, accompanied by changes in morphology. The histocompatibility association of mesenchymal stem cells from rabbit’s bone marrow, and PLDLA-TMC scaffolds, were evaluated in the meniscus regeneration, proving the potential of cell culture at in vivo tissue regeneration. Nine New Zealand rabbits underwent total medial meniscectomy, yielding three treatments: implantation of the seeded PLDLA-TMC scaffold, implantation of the unseeded PLDLA-TMC and negative control (defect without any implant). After 24 weeks, the results revealed the presence of fibrocartilage in the animals treated with polymer. However, the regeneration obtained with the seeded PLDLA-TMC scaffolds with mesenchymal stem cells had become intimal to mature fibrocartilaginous tissue of normal meniscus both macroscopically and histologically. This study demonstrated the effectiveness of the PLDLA-TMC scaffold in meniscus regeneration and the potential of mesenchymal stem cells in tissue engineering, without the use of growth factors. It is concluded that bioresorbable polymers represent a promising alternative for tissue regeneration.

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Section: Introductionmentioning

confidence: 99%