Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

Abstract

Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.

Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and regenerative (Foxn1 deficient; nude) mouse models

“…Excised skin tissues from newborn Balb/c and nude mice during five independent experiments (3–5 animals per experiment; total n = 20 Balb/c and n = 20 nude) were subjected to enzymatic digestion, according to a modified procedure described previously (Zuber et al 2014 ; Gawronska-Kozak et al 2016 ). In brief, to separate the epidermis from the underlying dermis, tissues were first digested with 6 U/ml dispase I (Life Technologies) overnight at 4 °C.…”

Changes in expression of cartilaginous genes during chondrogenesis of Wharton’s jelly mesenchymal stem cells on three-dimensional biodegradable poly(L-lactide-co-glycolide) scaffolds

“…Aliphatic polyesters such as polylactide (PLA), polyglycolide (PGA), poly-ɛ-caprolactone and its copolymers can degrade in contact with living tissue or under the influence of external factors. Biodegradation occurs as a result of their hydrolysis to lactic acid and glycolic acid [ 6 , 7 ]. Homopolymers themselves are not optimum materials for forming the 3D scaffolds for cartilage tissue regeneration.…”

“…PLGA has proved to be an excellent material for cartilage tissue engineering due to its biodegradable properties, mechanical strength, and ease of fabrication into a considerably complex formation. Moreover, PLGA is a synthetic material that has excellent biocompatibility [ 6 , 9 , 10 ].…”

The Effect of the New Lupeol Derivatives on Human Skin Cells as Potential Agents in the Treatment of Wound Healing

“…Human skin cells were isolated from skin biopsies taken from three healthy donors during plastic surgery and cultured as described previously [ 20 ]. Informed consent and Ethical Committee approval were obtained according to Polish law (No.…”