Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering

Narayanan, Ganesh; Vernekar, Varadraj N.; Kuyinu, Emmanuel; Laurencin, Cato T.

doi:10.1016/j.addr.2016.04.015

Cited by 391 publications

(272 citation statements)

References 345 publications

(301 reference statements)

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“…PLA was selected based on its extensive use as a fixation device in orthopedic repair and has been demonstrated suitable for fabrication of tissue engineered scaffolds. [74, 75] Alizarin red staining was performed on neat, cell-free scaffolds that had been soaked in growth media for 2 weeks for a qualitative assessment of calcium deposition. Fumarate-based chemistries have been shown to support surface mineralization when soaked in concentrated solutions of simulated body fluid.…”

Section: Resultsmentioning

confidence: 99%

Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels

et al. 2018

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The design of tissue engineered scaffolds based on polymerized high internal phase emulsions (polyHIPEs) has emerged as a promising bone grafting strategy. We previously reported the ability to 3D print emulsion inks to better mimic the structure and mechanical properties of native bone while precisely matching defect geometry. In the current study, redox-initiated hydrogel carriers were investigated for in situ delivery of human mesenchymal stem cells (hMSCs) utilizing the biodegradable macromer, poly(ethylene glycol)-dithiothreitol. Hydrogel carrier properties including network formation time, sol-gel fraction, and swelling ratio were modulated to achieve rapid cure without external stimuli and a target cell-release period of 5-7 days. These in situ carriers enabled improved distribution of hMSCs in 3D printed polyHIPE grafts over standard suspension seeding. Additionally, carrier-loaded polyHIPEs supported sustained cell viability and osteogenic differentiation of hMSCs post-release. In summary, these findings demonstrate the potential of this in situ curing hydrogel carrier to enhance the cell distribution and retention of hMSCs in bone grafts. Although initially focused on improving bone regeneration, the ability to encapsulate cells in a hydrogel carrier without relying on external stimuli that can be attenuated in large grafts or tissues is expected to have a wide range of applications in tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels

et al. 2018

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“…This aspect could be important because PLA membrane scaffolds synthesized by AJT were amorphous and the in vitro and in vivo degradability could be influenced by the fiber diameter reflected on the mechanical properties indicating changes in the scaffolds properties. However, the scaffolds exhibited dimensional stability during the degradation period time, which is an important prerequisite for materials evaluation for tissue engineering regeneration …”

Section: Discussionmentioning

confidence: 99%

In vitroandin vivobiological characterization of poly(lactic acid) fiber scaffolds synthesized by air jet spinning

Granados-Hernández

Serrano-Bello²,

Montesinos

et al. 2017

J Biomed Mater Res

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Poly(lactic acid) (PLA) is one of the most promising renewable and biodegradable polymers for mimic extracellular matrix for tissue engineering applications. In this work, PLA spun membrane scaffold were successfully prepared by air jet spinning technology. Morphology, mechanical properties, in vitro biocompatibility, and in vitro and in vivo degradation of PLA fibrous scaffold were characterized by X-ray diffraction, Fourier Transform Infrared, and scanning electron microscope (SEM). Morphological results assessed by SEM analyses indicated that PLA scaffolds possessed an average fiber diameter of approximately 0.558 ± 0.141 µm for 7% w/v of PLA and approximately 0.647 ± 0.137 µm for 10% w/v. Interestingly, our results showed that the nanofiber size of PLA scaffold allow structural stability after 100 days of in vitro degradation in Ringer solution where the average fiber diameter were of approximately 0.633 ± 0.147 µm for 7% w/v and approximately 0.645 ± 0.140 µm for 10% w/v of PLA. Mechanical properties of PLA fibers scaffold after in vitro degradation showed decrease in terms of flexibility elongation, and less energy was needed to achieve maximal elastic deformation. The fiber size exerts an influence on the biological response of human Bone Marrow Mesenchymal Stromal Cells as confirmed by MTT assay after 9 days of cell culture and the in vivo degradation assay of 7% w/v and 10% w/v of PLA scaffold, did not demonstrate evidence of toxicity with a mild inflammatory respond. In conclusion, airbrushing technology promises to be a viable and attractive alternative technique for producing a biocompatible PLA nanofiber scaffold that could be considered for tissue engineering regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2435-2446, 2018.

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“…Various synthetic and natural materials are currently being investigated for nucleus pulposus tissue engineering [21][22][23][24][25][26]. However, synthetic materials, such as chemically modified hyaluronan, can have toxic byproducts or result in a foreign body response [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering

et al. 2017

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Background Context: Disc degeneration is the leading cause of low back pain and is often characterized by a loss of disc height, resulting from cleavage of chondroitin sulfate proteoglycans (CSPGs) present in the nucleus pulposus. Intact CSPGs are critical to water retention and maintenance of the nucleus osmotic pressure. Decellularization of healthy nucleus pulposus tissue has the potential to serve as an ideal matrix for tissue engineering of the disc because of the presence of native disc proteins and CSPGs. Injectable in situ gelling matrices are the most viable therapeutic option to prevent damage to the anulus fibrosus and future disc degeneration.

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Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering

Cited by 391 publications

References 345 publications

Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels

Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels

In vitroandin vivobiological characterization of poly(lactic acid) fiber scaffolds synthesized by air jet spinning

Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering

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