Poly lactic acid (PLA) nanoparticles sustain the cytotoxic action of temozolomide in C6 Glioma cells

Jain, Darshana; Athawale, Rajani; Bajaj, Amrita; Shrikhande, Shruti; Goel, Peeyush N.; Nikam, Yuvraj; Gude, Rajiv P.

doi:10.1016/j.biomag.2013.08.003

Cited by 21 publications

(12 citation statements)

References 19 publications

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“…B16F10 cells were grown in 35 mm plates till they attained 60–70% confluency. Confluent cells were treated with plain dye and dye loaded nanocarriers for specific time limit as in a particular study (Jain et al, 2013). After harvesting, these cells were fixed using 1% PFA solution.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells

Shrikhande¹,

Jain²,

Athawale³

et al. 2015

Saudi Pharmaceutical Journal

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Nanoparticles are being increasingly used in the field of cancer treatment due to their unique properties and advantages. The aim of the present research work was to prepare and characterize a polymeric albumin nanosystem for Cisplatin and evaluate its in-vitro efficacy against B16F10 melanoma. The developed nanoparticles were almost spherical in shape with a particle size in the range of 150-300 nm, low polydispersity values and about 80% drug entrapment efficiency. Albumin nanocarriers sustained the release of Cisplatin for more than 48 h, suggesting the reduction in dosing schedule for this drug. The results from in-vitro cell line studies indicated the dose dependent cytotoxic potential of drug loaded albumin nanoparticles, their potential to inhibit cell proliferation and induce morphological changes. In addition, these nanoparticles exhibited superiority to Cisplatin in hampering the cell migration. Developed nanoparticles caused cell cycle arrest along with time and concentration dependent cellular uptake in B16F10 cell line. These results signify that the prepared Cisplatin albumin nanoparticles could serve as a promising approach for B16F10 melanoma treatment.

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Section: Methodsmentioning

confidence: 99%

Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells

Shrikhande¹,

Jain²,

Athawale³

et al. 2015

Saudi Pharmaceutical Journal

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“…Flow cytometric analysis was done to confirm our previous findings. Sub-confluent cultures of C6 glioma were treated with FITC and its loaded nanoparticles as done earlier [ 22 ]. Cells were thereafter harvested and fixed using 1% PFA.…”

Section: Methodsmentioning

confidence: 99%

Unraveling the cytotoxic potential of Temozolomide loaded into PLGA nanoparticles

et al. 2014

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BackgroundNanotechnology has received great attention since a decade for the treatment of different varieties of cancer. However, there is a limited data available on the cytotoxic potential of Temozolomide (TMZ) formulations. In the current research work, an attempt has been made to understand the anti-metastatic effect of the drug after loading into PLGA nanoparticles against C6 glioma cells.Nanoparticles were prepared using solvent diffusion method and were characterized for size and morphology. Diffusion of the drug from the nanoparticles was studied by dialysis method. The designed nanoparticles were also assessed for cellular uptake using confocal microscopy and flow cytometry.ResultsPLGA nanoparticles caused a sustained release of the drug and showed a higher cellular uptake. The drug formulations also affected the cellular proliferation and motility.ConclusionPLGA coated nanoparticles prolong the activity of the loaded drug while retaining the anti-metastatic activity.

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“…Clonogenic assay was performed to determine the colony formation inhibition activity of prepared nanoparticles [31]. A549 cells (500 cells/well in 2 mL medium) were seeded in 10 cm 2 plastic dishes.…”

Section: Clonogenic Assaymentioning

confidence: 99%

Natural polysaccharide functionalized gold nanoparticles as biocompatible drug delivery carrier

Pooja

Panyaram

Kulhari

et al. 2015

International Journal of Biological Macromolecules

124

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Poly lactic acid (PLA) nanoparticles sustain the cytotoxic action of temozolomide in C6 Glioma cells

Cited by 21 publications

References 19 publications

Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells

Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells

Unraveling the cytotoxic potential of Temozolomide loaded into PLGA nanoparticles

Natural polysaccharide functionalized gold nanoparticles as biocompatible drug delivery carrier

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