Poly-N-Acetyl Glucosamine Fibers Activate Bone Regeneration in a Rabbit Femur Injury Model

Muise‐Helmericks, Robin C.; Demcheva, Marina; Vournakis, John N.; Seth, Arun

doi:10.1097/ta.0b013e3182258799

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…In a more general view, single-chain amphiphile/sterol liposomes formed a versatile platform as several therapeutic or targeting components can be included. For example, SA was chosen in the present work to confer the positive surface charges and maintain the stability of the vesicles; however, a single-chain glucosamine may be a better amphiphile candidate to promote bone regeneration . Meanwhile, SA might be a good candidate to create a liposome surface that targets cancerous lesions.…”

Section: Resultsmentioning

confidence: 99%

“…For example, SA was chosen in the present work to confer the positive surface charges and maintain the stability of the vesicles; however, a single-chain glucosamine may be a better amphiphile candidate to promote bone regeneration. 44 Meanwhile, SA might be a good candidate to create a liposome surface that targets cancerous lesions. It is reported that many types of cancer cells display an elevated amount of phosphatidylserine on the luminal surface.…”

Section: Resultsmentioning

confidence: 99%

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Design and Characterization of a Therapeutic Non-phospholipid Liposomal Nanocarrier with Osteoinductive Characteristics To Promote Bone Formation

et al. 2017

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Sterosomes are a recently developed type of non-phospholipid liposomes formed from single-chain amphiphiles and high content of sterols. Although sterosomes presented significantly increased stability compared to conventional phospholipid liposomes, current sterosome biomaterials are not truly bioactive and have no intrinsic therapeutic effects. The purpose of this study was to develop a sterosome formulation with osteoinductive properties by an effective selection of sterol, one of the sterosome components. Oxysterols are oxidized derivatives of cholesterol and are known to stimulate osteogenesis and bone formation. Thus, 20S-hydroxycholesterol (Oxy), one of the most potent oxysterols for bone regeneration was examined as a promising candidate molecule to form fluid lamellar phases with a single-chain amphiphile, namely stearylamine (SA). First, the optimal composition was identified by investigating the phase behavior of SA/Oxy mixtures. Next, the capacity of the optimized SA/Oxy sterosomes to promote osteogenic differentiation of bone marrow stromal cells was assessed in vitro in a hydrogel environment. Furthermore, we explored the effects of osteogenic oxysterol sterosomes in vivo with the mouse critical-sized calvarial defect model. Our results showed that SA/Oxy sterosomes induced osteogenic differentiation in vitro and enhanced calvarial healing without delivery of additional therapeutic agents, indicating their intrinsic bone forming potential. This study suggests a promising non-phospholipid liposomal platform with osteoinductive properties for delivery of small molecular drugs and/or other therapeutic genes for enhanced bone formation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Design and Characterization of a Therapeutic Non-phospholipid Liposomal Nanocarrier with Osteoinductive Characteristics To Promote Bone Formation

et al. 2017

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“…Muise-Helmericks et al drilled circular holes (Φ 2.0 mm) in the femurs of rabbits and implanted n-chitin [ 150 ]. All the 15 n-chitin test sites were found to have new bone tissue present.…”

Section: Biomedical Applications Of Nano-chitinsmentioning

confidence: 99%

Emerging Biomedical Applications of Nano-Chitins and Nano-Chitosans Obtained via Advanced Eco-Friendly Technologies from Marine Resources

2014

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The present review article is intended to direct attention to the technological advances made in the 2010–2014 quinquennium for the isolation and manufacture of nanofibrillar chitin and chitosan. Otherwise called nanocrystals or whiskers, n-chitin and n-chitosan are obtained either by mechanical chitin disassembly and fibrillation optionally assisted by sonication, or by e-spinning of solutions of polysaccharides often accompanied by poly(ethylene oxide) or poly(caprolactone). The biomedical areas where n-chitin may find applications include hemostasis and wound healing, regeneration of tissues such as joints and bones, cell culture, antimicrobial agents, and dermal protection. The biomedical applications of n-chitosan include epithelial tissue regeneration, bone and dental tissue regeneration, as well as protection against bacteria, fungi and viruses. It has been found that the nano size enhances the performances of chitins and chitosans in all cases considered, with no exceptions. Biotechnological approaches will boost the applications of the said safe, eco-friendly and benign nanomaterials not only in these fields, but also for biosensors and in targeted drug delivery areas.

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“… 28 Also, short poly-Nacetyl glucosamine (sNAG) fibers have been shown to activate bone regeneration in rabbit femurs. 29 …”

Section: Introductionmentioning

confidence: 99%

The potential role of regenerative medicine in the management of traumatic patients

et al. 2014

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Abstract:Traumatic injury represents the most common cause of death in ages 1 to 44 years and a significant proportion of patients treated in hospital emergency wards each year. Unfortunately, for patients who survive their injuries, survival is not equal to complete recovery. Many traumatic injuries are difficult to treat with conventional therapy and result in permanent disability. In such situations, regenerative medicine has the potential to play an important role in recovery of function. Regenerative medicine is a field that seeks to maintain or restore function with the development of biological substitutes for diseased or damaged tissues. Several regenerative approaches are currently under investigation, with a few achieving clinical application. For example, engineered skin has gained FDA approval, and more than 20 tissue engineered skin substitutes are now commercially available. Other organ systems with promising animal models and small human series include the central and peripheral nervous systems, the musculoskeletal system, the respiratory and genitourinary tracts, and others. This paper will be a clinically oriented review of the regenerative approaches currently under investigation of special interest to those caring for traumatic patients.

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Poly-N-Acetyl Glucosamine Fibers Activate Bone Regeneration in a Rabbit Femur Injury Model

Abstract: The sNAG membrane test material activated the regeneration of new bone tissue in a rabbit femur bone model after 28 days of implantation, whereas the control bone wax material did not.

Cited by 6 publications

References 7 publications

Design and Characterization of a Therapeutic Non-phospholipid Liposomal Nanocarrier with Osteoinductive Characteristics To Promote Bone Formation

Design and Characterization of a Therapeutic Non-phospholipid Liposomal Nanocarrier with Osteoinductive Characteristics To Promote Bone Formation

Emerging Biomedical Applications of Nano-Chitins and Nano-Chitosans Obtained via Advanced Eco-Friendly Technologies from Marine Resources

The potential role of regenerative medicine in the management of traumatic patients

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