Poly(N-isopropylacrylamide) co-polymer films as potential vehicles for delivery of an antimitotic agent to vascular smooth muscle cells

Doorty, Kevina B.; Golubeva, T.; Gorelov, Alexander; Rochev, Yuri; Allen, Lorcan T.; Dawson, Kenneth A.; Gallagher, William M.; Keenan, A. K.

doi:10.1016/s1054-8807(02)00165-5

Cited by 68 publications

(49 citation statements)

References 16 publications

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“…Copolymer films of PNIPAAm and poly(N-butylacrylamide) were shown by Wilson et al and Doorty et al to give a sustained release of drugs from the film over a considerable time period [3,126]. They showed the released amounts of drug loaded at room temperature to be inversely proportional to the hydrophobic monomer content once heated to 37 °C [3,126].…”

Section: Filmsmentioning

confidence: 99%

“…Most of the studies discussed in this review use polymers that present an LCST. The most common of these is poly(N-isopropylacrylamide) (PNIPAAm) [1][2][3][4]. PNIPAAm has a lower critical solution temperature, LCST of around 32 °C, a very useful temperature for biomedical applications since it is close to the body temperature (37 °C).…”

Section: Open Accessmentioning

confidence: 99%

“…PNIPAAm has a lower critical solution temperature, LCST of around 32 °C, a very useful temperature for biomedical applications since it is close to the body temperature (37 °C). Adjustment of the LCST of PNIPAAm has been achieved by copolymerizing with hydrophilic or hydrophobic monomers rendering the overall hydrophilicity of the polymer higher or lower respectively [3,5,6,15]. Other polymers with thermoresponsive properties include poly(N,N-diethylacrylamide) (PDEAAm) with an LCST over the range of 25 to 32 °C, poly(N-vinlycaprolactam) (PVCL) [7,8,16] with an LCST between 25 and 35 °C, poly [2-(dimethylamino)ethyl methacrylate] (PDMAEMA) [17][18][19][20][21][22] with an LCST of around 50 °C [21] and poly(ethylene glycol) (PEG), also called poly(ethylene oxide) (PEO) whose LCST is around 85 °C [23][24][25][26].…”

Section: Open Accessmentioning

confidence: 99%

“…"Smart" materials, i.e., materials that have the ability to respond to external stimuli, represent one of the most exciting and immerging class of materials [1][2][3][4][5][6][7][8][9]. One of the main groups of "smart" materials is based on polymers because they are cheaper and more easily tailored than metals or ceramics.…”

Section: Introductionmentioning

confidence: 99%

“…One of the main groups of "smart" materials is based on polymers because they are cheaper and more easily tailored than metals or ceramics. These polymeric "smart" materials can respond to stimuli such as pH, temperature, ionic strength, electric or magnetic field, light and/or chemical and biological stimuli and consequently have a wide range of applications that include sensors, drug delivery, gene delivery and tissue engineering [1][2][3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Thermoresponsive Polymers for Biomedical Applications

2011

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Thermoresponsive polymers are a class of "smart" materials that have the ability to respond to a change in temperature; a property that makes them useful materials in a wide range of applications and consequently attracts much scientific interest. This review focuses mainly on the studies published over the last 10 years on the synthesis and use of thermoresponsive polymers for biomedical applications including drug delivery, tissue engineering and gene delivery. A summary of the main applications is given following the different studies on thermoresponsive polymers which are categorized based on their 3-dimensional structure; hydrogels, interpenetrating networks, micelles, crosslinked micelles, polymersomes, films and particles.

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Section: Filmsmentioning

confidence: 99%

Section: Open Accessmentioning

confidence: 99%

Section: Open Accessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thermoresponsive Polymers for Biomedical Applications

2011

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Stimulation‐Sensitive Drug Delivery Systems

Shuai

Cheng

2014

Bioinspired and Biomimetic Polymer Systems for Drug and Gene Delivery

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Extended delivery of the antimitotic agent colchicine from thermoresponsive N‐isopropylacrylamide‐based copolymer films to human vascular smooth muscle cells

Wilson

Gorelov

Rochev

et al. 2003

J Biomedical Materials Res

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The aim of this study was to establish the capacity of thermoresponsive poly(N-isopropylacrylamide) copolymer films to deliver bioactive concentrations of an antimitotic agent to human vascular smooth muscle cells (HASMC) over an extended period of time. Copolymer films were prepared using a 50:50 (w/w) ratio of N-isopropylacrylamide (NiPAAm) monomer to the more hydrophobic N-tert-butylacrylamide (NtBAAm) and loaded with the antimitotic agent colchicine (0.1 micromol per film) at room temperature. Colchicine release from films was sustained over a 14-day period. At 24 h postloading, the concentration of colchicine in the medium overlying films was 2.12 +/- 0.16 microM; this fell to 0.20 +/- 0.01 microM at 7 days and decreased further to 0.12 +/- 0.01 microM after 14 days. Colchicine released from copolymer films inhibited proliferation when subsequently placed on HASMC: at 0.1 microM, released colchicine reduced proliferation to 18.5 +/- 0.8% of control cells (p < 0.001, n = 9). The antiproliferative effect of released colchicine was comparable to that of native colchicine, as observed in separate experiments. Furthermore, colchicine released from 50:50 polymer films inhibited the proliferation of cells grown in the same environment as the copolymer. Inhibition of cell proliferation was not due to the release of cytotoxic particles from the copolymer because medium incubated with copolymer for 5 days and then applied to HASMC did not alter cell viability. In conclusion, this study demonstrates that 50:50 NiPAAm:NtBAAm copolymers can deliver bioactive concentrations of the antimitotic agent colchicine to human vascular cells over an extended period of time.

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Poly(N-isopropylacrylamide) co-polymer films as potential vehicles for delivery of an antimitotic agent to vascular smooth muscle cells

Cited by 68 publications

References 16 publications

Thermoresponsive Polymers for Biomedical Applications

Thermoresponsive Polymers for Biomedical Applications

Stimulation‐Sensitive Drug Delivery Systems

Extended delivery of the antimitotic agent colchicine from thermoresponsive N‐isopropylacrylamide‐based copolymer films to human vascular smooth muscle cells

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