Poly(N-isopropylacrylamide)-graft-polypropylene membranes containing adsorbed antibody for cell separation

Okamura, Ai; Itayagoshi, Midori; Hagiwara, Taeko; Yamaguchi, Manae; Kanamori, Toshiyuki; Shinbo, Toshio; Wang, Pichao

doi:10.1016/j.biomaterials.2004.04.028

Cited by 55 publications

(25 citation statements)

References 20 publications

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“…Recently, PNIPAM-based stimuli-responsive surfaces have received considerable attention because of their potential applications in many fields, including drug release [19][20][21], tissue engineering [22][23][24], sensing [25], and smart separation [26][27][28][29][30][31]. These applications mainly rely on the responsive wettability of PNIPAMmodified surfaces [32].…”

Section: Introductionmentioning

confidence: 99%

Thermo-responsive stick-slip behavior of advancing water contact angle on the surfaces of poly(N-isopropylacrylamide)-grafted polypropylene membranes

et al. 2010

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Wettability of a solid surface is highly important to its practical application, especially for the surface that shows thermoresponsive properties. In this paper, we describe a thermo-responsive stick-slip behavior of water droplets on the surfaces of poly(N-isopropylacrylamide) (PNIPAM)-grafted polypropylene membranes. Field emission scanning electron microscope (FESEM) images elucidate that the morphology of PNIPAM-grafted membrane surface is thermo-responsive, i.e., the surface becomes rougher above the lower critical solution temperature (LCST) of PNIPAM. On the surface of nascent polypropylene membranes, the water droplet shows a smooth motion resulting in advancing and receding water contact angles of 111° and ~65°, respectively. On the PNIPAM-grafted membrane surfaces, the water droplet shows a stick-slip pattern above the LCST, whereas it advances smoothly below the LCST. This phenomenon is reproducible and can be ascribed to the energy barriers enhanced by the shrink of PNIPAM chains above the LCST. We also find that the slip contact angle decreases from 102° to 92° after several stick-slip cycles. This decrease is attributed to the water adsorption on the grafted PNIPAM layer, which is confirmed by the continuous decrease of the receding water contact angle. stick-slip behavior, water contact angle, thermo-responsive surface, poly(N-isopropylacrylamide), polypropylene membrane

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Section: Introductionmentioning

confidence: 99%

Thermo-responsive stick-slip behavior of advancing water contact angle on the surfaces of poly(N-isopropylacrylamide)-grafted polypropylene membranes

et al. 2010

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“…In recent years, increased attention has been paid to hydrogels and polymers that display controlled changes in volume in response to small changes in environmental factors such as temperature, pH, ionic strength, and electric field. Polymers that are sensitive to temperature, pH, and electric fields have been suggested for use in biomedical1–10 and biotechnology systems, e.g., intelligent drug release systems for immobilization of enzymes11 and other biocompounds,12 and in separation processes 13,14. Poly( N ‐isopropylacrylamide) is one of the most studied of such polymers, which shows a reversible, thermosensitive‐phase transition in aqueous solution 15–17.…”

Section: Introductionmentioning

confidence: 99%

Temperature and pH‐Sensitive Swelling Behavior of Binary DMAEMA/4VP Grafts on Poly(propylene) Films

Burillo

Bucio

Arenas

et al. 2007

Macro Materials & Eng

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Poly(N,N‐dimethylaminoethyl methacrylate) (PDMAEMA) is a polymer or hydrogel that is both thermosensitive and pH sensitive, with a low critical solution temperature (LCST) around 38 °C and a pH critical point of 2.5. Poly(4‐vinylpyridine) (P4VP) shows pH sensitivity with a critical point of 5.1. Grafting of stimuli‐sensitive polymers onto mechanically durable poly(propylene) (PP) substrates was used in this study. We have focused on the influence of temperature and pH on the response of binary graft films produced by gamma irradiation in one and two steps. An LCST‐type hydration transition in the grafts was observed by measuring swelling of the films and water contact angle at different temperatures and pH. An upper critical solution temperature (UCST)‐type behavior was also observed by swelling PP‐g‐DMAEMA and DMAEMA/4VP binary grafting onto PP films at pH 2.2.magnified image

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“…[9] In addition, membranes with functional surfaces constructed by various chemical/physical methods have been applied for the concentration/separation of proteins [10,11] and cells. [12] In our previous work, the glycosylation of microporous poly(propylene) membranes (MPPMs) was realized by either 'surface grafting' or 'surface immobilization' strategies. [13] The former is based on the synthesis of saccharide derivatives with a vinyl group and the subsequent formation of grafting glycopolymers on the membrane surfaces.…”

Section: à2mentioning

confidence: 99%

High‐Density Glycosylation of Polymer Membrane Surfaces by Click Chemistry for Carbohydrate–Protein Recognition

Wang

2010

Macromol. Rapid Commun.

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To biologically mimic the carbohydrate-protein interactions in artificial systems, one of the challenges is to construct a glycosylated surface with a high glycosyl density to yield a notable 'glycoside cluster effect'. A novel strategy is presented for high density glycosylation of the surface of a microporous poly(propylene) membrane (MPPM) by click chemistry. It is promising that the surface glycosyl density can be well controlled over a wide range and the maximum value is over 10 µmol · cm(-2) . The recognition capability of these glycosylated MPPMs to lectins indicates the occurrence of the 'glycoside cluster effect' when the glycosyl density on the membrane surface exceeds 0.20 µmol · cm(-2) .

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Poly(N-isopropylacrylamide)-graft-polypropylene membranes containing adsorbed antibody for cell separation

Cited by 55 publications

References 20 publications

Thermo-responsive stick-slip behavior of advancing water contact angle on the surfaces of poly(N-isopropylacrylamide)-grafted polypropylene membranes

Thermo-responsive stick-slip behavior of advancing water contact angle on the surfaces of poly(N-isopropylacrylamide)-grafted polypropylene membranes

Temperature and pH‐Sensitive Swelling Behavior of Binary DMAEMA/4VP Grafts on Poly(propylene) Films

High‐Density Glycosylation of Polymer Membrane Surfaces by Click Chemistry for Carbohydrate–Protein Recognition

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