Poly(NaSS) Functionalization Modulates the Conformation of Fibronectin and Collagen Type I To Enhance Osteoblastic Cell Attachment onto Ti6Al4V

Abstract

Functionalization of surfaces with poly(sodium styrenesulfonate) (poly(NaSS)) has recently been found to enhance osteointegration of implantable materials. Radical polymerization of poly(NaSS) on titanium (Ti)-based substrates has been used to improve their long-term performance by preventing fibrosis and consequently implant loosening. However, the influence of the sulfonate groups on the early cell behavior and the associated molecular phenomena remains to be understood. In this work, we used quartz crystal microbalance with dissipation (QCM-D) to elucidate the role of poly(NaSS) in enhancing osteoblastic cell attachment. This was measured by following the cell attachment using the MC3T3-E1 cell line, on fetal bovine serum (FBS) preadsorbed surfaces and on substrates adsorbed with a series of relevant proteins, bovine serum albumin (BSA), fibronectin (Fn), and collagen type I (Col I). Comparison of the performance of poly(NaSS) with other clinically important substrates such as Ti alloy Ti6Al4V, gold, and poly(desamino-tyrosyl-tyrosine ethyl ester carbonate) (poly(DTEc)) indicates poly(NaSS) to be a superior substrate for MC3T3-E1 cells attachment. This attachment was found to be integrin mediated in the presence of Fn and Col I. Antibodies specific to the RGD peptide and the N- and C-terminal HB-binding domains reacted more intensively with Fn adsorbed on poly(NaSS). Fn adapts a conformation favorable to RGD mediated cell attachment when adsorbed onto poly(NaSS).

Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging

“…Our result suggests that DNA was not affected by the poly(AAm‐co‐SS) hydrogel. However, there is concern that proteins might be affected by the polymer, because some previous reports showed protein denaturation by poly(sodium p ‐styrenesulfonate) . In this study, poly(AAm‐co‐SS) hydrogels with high refractive index were used as a polyelectrolyte gel, but the contribution of poly(AAm‐co‐SS) to the refractive index was limited.…”

“…However, there is concern that proteins might be affected by the polymer, because some previous reports showed protein denaturation by poly(sodium p-styrenesulfonate). 28,29 In this study, poly(AAm-co-SS) hydrogels with high refractive index were used as a polyelectrolyte gel, but the contribution of poly(AAm-co-SS) to the refractive index was limited. We are currently conducting studies of the optical tissue clearing using other anionic monomers for their application to threedimensional GFP imaging.…”

Analysis of early cellular responses of anterior cruciate ligament fibroblasts seeded on different molecular weight polycaprolactone films functionalized by a bioactive poly(sodium styrene sulfonate) polymer

“…Some scientists seek to immobilize directly extracellular matrix (ECM) proteins to the surface including fibronectin (Fn) and hyaluronic acid [5], [12], [13] while others use only specific sequences of proteins as the RGD sequences [14], [15] and some use synthetic biomolecules [16]- [18]. With the aim of ligament reconstruction and in continuity of already developed bioactive synthetic ligament [19]- [21], the current study focused on the functionalization of PCL surfaces by poly(sodium styrene sulfonate) (pNaSS), a bioactive polymer that was demonstrated [16][17][18][19][20][21], [47][48][49][50] to endow surfaces with bioactive properties as the modulation of adhesive protein conformations, the modulation of cell and bacteria adhesion and the improvement of in vivo biointegration.…”

Enhancement of plasma protein adsorption and osteogenesis of hMSCs by functionalized siloxane coatings for titanium implants

“…It has structural functions and interacts with cells via integrin receptors and with other ECM proteins, for example, Fn . The adsorption behavior of these proteins were correlated with surface properties such as wettability, topography, and functionality that are known to affect biological response …”