(Poly)phenolic fingerprint and chemometric analysis of white (Morus alba L.) and black (Morus nigra L.) mulberry leaves by using a non-targeted UHPLC–MS approach

Sánchez-Salcedo, Eva M.; Tassotti, Michele; Rio, Daniele Del; Hernández, Francisca; Martínez, Juan José; Mena, Pedro

doi:10.1016/j.foodchem.2016.05.121

Cited by 111 publications

(63 citation statements)

References 28 publications

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“…alba and M . nigra which revealed that flavonols were the most relevant and caffeoylquinic acids are the phenolic compounds present in larger quantities in mulberry leaves, being chlorogenic acid (5-caffeoylquinic acid) the predominant one [ 63 ]. In a previous study, Sánchez-Salcedo et al (2015) found that the predominant flavonols in mulberry leaves of M .…”

Section: Resultsmentioning

confidence: 99%

Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity

Freitas¹,

Fontes²,

Souza³

et al. 2016

PLoS ONE

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Melanogenesis is a process responsible for melanin production, which is stored in melanocytes containing tyrosinase. Inhibition of this enzyme is a target in the cosmetics industry, since it controls undesirable skin conditions such as hyperpigmentation due to the overproduction of melanin. Species of the Morus genus are known for the beneficial uses offered in different parts of its plants, including tyrosinase inhibition. Thus, this project aimed to study the inhibitory activity of tyrosinase by extracts from Morus nigra leaves as well as the characterization of its chromatographic profile and cytotoxicity in order to become a new therapeutic option from a natural source. M. nigra leaves were collected, pulverized, equally divided into five batches and the standardized extract was obtained by passive maceration. There was no significant difference between batches for total solids content, yield and moisture content, which shows good reproducibility of the extraction process. Tyrosinase enzymatic activity was determined for each batch, providing the percentage of enzyme inhibition and IC50 values obtained by constructing dose-response curves and compared to kojic acid, a well-known tyrosinase inhibitor. High inhibition of tyrosinase activity was observed (above 90% at 15.625 μg/mL). The obtained IC50 values ranged from 5.00 μg/mL ± 0.23 to 8.49 μg/mL ± 0.59 and were compared to kojic acid (3.37 μg/mL ± 0.65). High Performance Liquid Chromatography analysis revealed the presence of chlorogenic acid, rutin and, its major compound, isoquercitrin. The chromatographic method employed was validated according to ICH guidelines and the extract was standardized using these polyphenols as markers. Cytotoxicity, assessed by MTT assay, was not observed on murine melanomas, human keratinocytes and mouse fibroblasts in tyrosinase IC50 values. This study demonstrated the potential of M. nigra leaf extract as a promising whitening agent of natural source against skin hyperpigmentation.

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Section: Resultsmentioning

confidence: 99%

Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity

Freitas¹,

Fontes²,

Souza³

et al. 2016

PLoS ONE

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“…Compound 34 fragmented to m/z 609 (loss of hexoside) and 301 (loss of rutinoside-hexoside). Regarding kaempferol derivatives, compounds 33, 42, 48, and 59 had already been described in mulberry fruits (Juan et al, 2012;Natić et al, 2015;, while kaempferol-rutinoside-hexoside (22) and kaempferol-rutinoside (39) have been recently identified in mulberry leaves (Dugo et al, 2009;Sánchez-Salcedo, Tassotti, Del Rio, Hernández, Martínez, & Mena, 2016), but not in fruits. Some flavonols reported for Serbian mulberry fruits (Natić et al, 2015) were not detected in the present study, which could account for geographical and genotypic differences.…”

Section: Identification Of Phytochemicals Compounds In Mulberry Fruitsmentioning

confidence: 99%

Phytochemical evaluation of eight white (Morus alba L.) and black (Morus nigra L.) mulberry clones grown in Spain based on UHPLC-ESI-MSn metabolomic profiles

Mena

Sánchez-Salcedo

Tassotti

et al. 2016

Food Research International

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“…In addition, nuciferine from N. nucifera leaf ameliorated hepatic steatosis in high-fat diet/streptozocin-induced diabetic mice [ 15 ]. It was reported that flavonoids from Nelumbo nucifera leaves, including quercetin, catechin, hyperoside, isoquercitrin, and astragalin, exhibit lipolytic activity and have hypolipidemic effects on adipose tissue [ 16 , 17 ]. Various studies have demonstrated that bioactive constituents such as isoquercitrin, chlorogenic, acid and rutin from M. alba (Mulberry) leaves ameliorate hyperglycemia in type 2 diabetes [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Preventive Effect of Flavonol Derivatives Abundant Sanglan Tea on Long-Term High-Fat-Diet-Induced Obesity Complications in C57BL/6 Mice

Guruvaiah

Guo

et al. 2018

Nutrients

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Sanglan Tea (SLT) is a Chinese medicine-based formulation that is consumed as a health drink for the effective management of obesity-associated complications. However, its chemical components and mechanism of action in the prevention of hepatic steatosis and obesity-related impairments have been uncertain. In this study, we aimed to unveil the chemical profile of SLT and to explore its preventive mechanism in high-fat-diet-induced non-alcoholic fatty liver disease (NAFLD) and obesity-related consequences in C57BL/6 mice. Ultrahigh-performance liquid chromatography (UHPLC) coupled to a quadrupole-orbitrap high-resolution mass spectrometry (MS) analysis of SLT indicated that analogs of quercetin and kaempferol are major compounds of flavonoids in SLT. A dietary supplement of SLT efficiently managed the blood glucose elevation, retained the serum total cholesterol (TC), LDL-cholesterol (LDL-C), and triglyceride (TG) levels, as well as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, and reduced the fat storage in the liver induced by a high-fat diet. The underlying mechanism of this preventive effect is hypothesized to be related to the inhibition of over-expression of lipogenesis and adipogenesis-related genes. Overall, this study suggests that SLT, being rich in quercetin and kaempferol analogs, could be a potential food supplement for the prevention of high-fat-diet-induced NAFLD and obesity-related complications.

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(Poly)phenolic fingerprint and chemometric analysis of white (Morus alba L.) and black (Morus nigra L.) mulberry leaves by using a non-targeted UHPLC–MS approach

Cited by 111 publications

References 28 publications

Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity

Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity

Phytochemical evaluation of eight white (Morus alba L.) and black (Morus nigra L.) mulberry clones grown in Spain based on UHPLC-ESI-MSn metabolomic profiles

Preventive Effect of Flavonol Derivatives Abundant Sanglan Tea on Long-Term High-Fat-Diet-Induced Obesity Complications in C57BL/6 Mice

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