Poly(<scp>ADP</scp>‐ribose) glycohydrolase silencing‐mediated <scp>H2B</scp> expression inhibits benzo(a)pyrene‐induced carcinogenesis

Zeng, Zhuoying; Lu, Jingjing; Wu, Desheng; Zuo, Ran; Li, Yuxi; Huang, Haiyan; Yuan, Jun; Hu, Zhangli

doi:10.1002/tox.23034

Cited by 18 publications

(16 citation statements)

References 15 publications

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“…The expression levels of the KoWRKY genes were analyzed in various tissues, including root, stem, leaf, flower, pistil, stamen, sepal, and fruit tissues. The expression data were transformed into log 2 [transcripts per million (TPM)+1] values for differential expression analysis ( Zeng et al, 2020 ). The resulting gene expression profiles were visualized as a heatmap via TBtools software ( Chen et al, 2020a ).…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of WRKY Genes and Their Responses to Chilling Stress in Kandelia obovata

You

Zhao³

et al. 2022

Front. Genet.

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Background:Kandelia obovata, a dominant mangrove species, is widely distributed in tropical and subtropical areas. Low temperature is the major abiotic stress that seriously limits the survival and growth of mangroves. WRKY transcription factors (TFs) play vital roles in responses to biotic and abiotic stresses. However, genome-wide analysis of WRKY genes in K. obovata and their responses to chilling stress have not been reported.Methods: Bioinformatic analysis was used to identify and characterize the K. obovata WRKY (KoWRKY) gene family, RNA-seq and qRT–PCR analyses were employed to screen KoWRKYs that respond to chilling stress.Results: Sixty-four KoWRKYs were identified and they were unevenly distributed across all 18 K. obovata chromosomes. Many orthologous WRKY gene pairs were identified between Arabidopsis thaliana and K. obovata, showing high synteny between the two genomes. Segmental duplication events were found to be the major force driving the expansion for the KoWRKY gene family. Most of the KoWRKY genes contained several kinds of hormone- and stress-responsive cis-elements in their promoter. KoWRKY proteins belonged to three groups (I, II, III) according to their conserved WRKY domains and zinc-finger structure. Expression patterns derived from the RNA-seq and qRT–PCR analyses revealed that 9 KoWRKYs were significantly upregulated during chilling acclimation in the leaves. KEGG pathway enrichment analysis showed that the target genes of KoWRKYs were significantly involved in 11 pathways, and coexpression network analysis showed that 315 coexpressed pairs (KoWRKYs and mRNAs) were positively correlated.Conclusion: Sixty-four KoWRKYs from the K. obovata genome were identified, 9 of which exhibited chilling stress-induced expression patterns. These genes represent candidates for future functional analysis of KoWRKYs involved in chilling stress related signaling pathways in K. obovata. Our results provide a basis for further analysis of KoWRKY genes to determine their functions and molecular mechanisms in K. obovata in response to chilling stress.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of WRKY Genes and Their Responses to Chilling Stress in Kandelia obovata

You

Zhao³

et al. 2022

Front. Genet.

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“…The 17 genes used to establish the d-signature comprised 16 protein-coding genes and one lncRNA gene, all of which were upregulated in CRC samples. The H2BFS expression level in lung cancer tissue has been reported to be higher than that in normal lung tissue ( 29 ). However, its expression in CRC remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Plasma Extracellular Vesicle Long RNAs Have Potential as Biomarkers in Early Detection of Colorectal Cancer

et al. 2022

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BackgroundEarly detection of colorectal cancer (CRC) is crucial to the treatment and prognosis of patients. Traditional screening methods have disadvantages.Methods231 blood samples were collected from 86 CRC, 56 colorectal adenoma (CRA), and 89 healthy individuals, from which extracellular vesicle long RNAs (exLRs) were isolated and sequenced. An CRC diagnostic signature (d-signature) was established, and prognosis-associated cell components were evaluated.ResultsThe exLR d-signature for CRC was established based on 17 of the differentially expressed exLRs. The d-signature showed high diagnostic efficiency of CRC and control (CRA and healthy) samples with an area under the curve (AUC) of 0.938 in the training cohort, 0.943 in the validation cohort, and 0.947 in an independent cohort. The d-signature could effectively differentiate early-stage (stage I–II) CRC from healthy individuals (AUC 0.990), as well as differentiating CEA-negative CRC from healthy individuals (AUC 0.988). A CRA d-signature was also generated and could differentiate CRA from healthy individuals both in the training (AUC 0.993) and validation (AUC 0.978) cohorts. The enrichment of class-switched memory B-cells, B-cells, naive B-cells, and mast cells showed increasing trends between CRC, CRA, and healthy cohorts. Class-switched memory B-cells, mast cells, and basophils were positively associated with CRC prognosis while natural killer T-cells, naive B-cells, immature dendritic cells, and lymphatic endothelial cells were negatively associated with prognosis.ConclusionsOur study identified that the exLR d-signature could differentiate CRC from CRA and healthy individuals with high efficiency and exLR profiling also has potential in CRA screening and CRC prognosis prediction.

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“…A mutation in histone H2B, as a new mechanism of epigenetic dysfunction, occurs in diverse cancers and represents a new class of oncogenic drivers [13,14]. A recent study demonstrated the increased levels of several subtypes of histone H2B after BaP-induced malignant cell transformation, and PARG silencing could inhibit BaP-induced carcinogenesis by downregulating the expression of H2B, indicating the important role of H2B in oncogenesis [15]. Previous bioinformatic studies showed the high expression level of HIST1H2BK in various types of cancer, which was associated with poor prognosis [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK

Huang¹,

Li²,

Zhao³

et al. 2022

J. Cancer

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Background: FAM110A belongs to the FAM110 family, which mainly functions in biological processes associated with the cell cycle. However, the biological functions in which FAM110A participates are largely undefined. In particular, its potential role in cancer remains unknown. The goal of this study was to uncover the role and mechanism of FAM110A in pancreatic cancer. Methods: Based on bioinformatics databases, qPCR and Western blot assays, we verified the elevated expression level of FAM110A in PDAC. Subsequently, FAM110A, HIST1H2BK and TSPAN1 overexpression or knockdown stable transfected cells were employed for biological functions' studies to explore the role in PDAC in vitro and in vivo. RNA-Seq, Western blot and luciferase-reporter assays were used to explore mechanism of FAM110A action in PDAC, and the involved pathway was verified by tumor phenotypic rescue experiments. Results: In this study, we demonstrated for the first time that FAM110A is an oncogene that promotes cell proliferation, migration, invasion and tumorigenesis in pancreatic cancer. HIST1H2BK was identified as the downstream target of FAM110A, while the promotion effect caused by FAM110A overexpression could be abolished by HIST1H2BK knockdown. Moreover, for the first time, we revealed the oncogenic role of HIST1H2BK in pancreatic cancer, and the tumor-promoting capacity of HIST1H2BK may be associated with its regulatory effect on G9a. In addition, we demonstrated that TSPAN1 displayed a positive transcriptional regulatory effect on FAM110A. Conclusions: Collectively, FAM110A plays an oncogenic role in PDAC, and the newly identified TSPAN1/FAM110A/HIST1H2BK/G9a pathway is involved in the modulation of pancreatic cancer progression and provides a novel prognostic and therapeutic strategy for pancreatic cancer treatment.

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Poly(ADP‐ribose) glycohydrolase silencing‐mediated H2B expression inhibits benzo(a)pyrene‐induced carcinogenesis

Cited by 18 publications

References 15 publications

Genome-Wide Identification of WRKY Genes and Their Responses to Chilling Stress in Kandelia obovata

Genome-Wide Identification of WRKY Genes and Their Responses to Chilling Stress in Kandelia obovata

Plasma Extracellular Vesicle Long RNAs Have Potential as Biomarkers in Early Detection of Colorectal Cancer

TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK

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