Poly-ß-hydroxybutyrate content and dose of the bacterial carrier for Artemia enrichment determine the performance of giant freshwater prawn larvae

Abstract

The beneficial effects of poly-β-hydroxybutyrate (PHB) for aquaculture animals have been shown in several studies. The strategy of applying PHB contained in a bacterial carrier has, however, hardly been considered. The effect of administering PHB-accumulated Alcaligenes eutrophus H16 containing 10 or 80 % PHB on dry weight, named A10 and A80, respectively, through the live feed Artemia was investigated on the culture performance of larvae of the giant freshwater prawn (Macrobrachium rosenbergii). Feeding larvae with Artemia nauplii enriched in a medium containing 100 and 1,000 mg L(-1) A80 significantly increased the survival with about 15 % and the development of the larvae with a larval stage index of about 1 as compared to feeding non-enriched Artemia. The survival of the larvae also significantly increased with about 35 % in case of a challenge with Vibrio harveyi. The efficiency of these treatments was equal to a control treatment of Artemia enriched in an 800 mg L(-1) PHB powder suspension, while Artemia enriched in 10 mg L(-1) A80, 100 mg L(-1) A10, and 1,000 mg L(-1) A10 did not bring similar effects. From our results, it can be concluded that PHB supplemented in a bacterial carrier (i.e., amorphous PHB) can increase the larviculture efficiency of giant freshwater prawn similar to supplementation of PHB in powdered form (i.e., crystalline PHB). When the level of PHB in the bacterial carrier is high, similar beneficial effects can be achieved as crystalline PHB, but at a lower live food enrichment concentration expressed on PHB basis.

Immunostimulatory effects of dietary poly-β-hydroxybutyrate in European sea bass postlarvae

“…However, in the present study, none of the estimated growth‐related parameters such as total length, dry weight and Fulton's condition factor K were affected by the PHB treatment. In Siberian sturgeon larvae, dietary PHB decreased growth (Najdegerami et al., ), whereas it increased growth in giant freshwater prawn larvae, Chinese mitten crab larvae and sea bass juveniles (De Schryver et al., ; Nhan et al., ; Sui et al., ; Thai et al., ), while no effect was observed on larval size in blue mussels (Hung et al., ). Generally, the effect of PHB on larval survival and growth performance seems to be species‐specific as well as depend on the onset, dose and duration of the PHB supplementation and the developmental stage of the investigated organism.…”

“…The bacteria had a PHB content of 75% of the cell dry weight and were produced as described in Thai et al. ().…”

“…The PHB solution consisted of freeze-dried PHB-accumulated bacteria (Alcaligenes eutrophus) dissolved in UV-treated salt water (salinity: 32 g/L) at a concentration of 10 8 bacteria/ml. The bacteria had a PHB content of 75% of the cell dry weight and were produced as described in Thai et al (2014).…”

Poly-beta-hydroxybutyrate-enrichedArtemiasp. for giant tiger prawnPenaeus monodonlarviculture

“…) or bioencapsulated in Artemia (Thai et al . ) protected the postlarvae of P. monodon and larvae of M. rosenbergii , respectively. Lipid supplementation alone or the combined supplementation of lipids and PHB did not result in an increased resistance of the postlarvae.…”

Microbial Poly‐3‐Hydroxybutyrate (PHB) as a Feed Additive for Fishes and Piglets

“…JL47 significantly enhanced the innate immunity of postlarvae of the crab . PHB was also found to improve the survival of the larvae of the prawn Macrobrachium rosenbergii and the blue mussel Mytilus edulis , as well as the juveniles of the Nile tilapia Oreochromis niloticus . Additionally, PHB not only affects marine organisms, but the feed composition shapes the intestinal bacterial communities and affects the health of large livestock …”