Poly(vinyl chloride) tubing with covalently bound alkaline phosphatase and alternative approach for investigations of open-tubular bioreactors

Rozum, Beata; Gajownik, Kamil; Tymecki, Łukasz; Koncki, Robert

doi:10.1016/j.ab.2010.01.016

Cited by 16 publications

(6 citation statements)

References 13 publications

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“…ALP was covalently immobilized on silicon surfaces by Ehlert et al143 Different trimethoxysilane linkers were used of which the aminopropyl trimethoxysilane gave the best results. To coat open‐tubular bioreactors the carboxyl‐groups of carboxylated poly(vinyl chloride) (PVC) were activated via carbodiimide chemistry by Rozum et al followed by attachment of ALP onto the EDC–NHS activated PVC surface 144. Using a similar carbodiimide‐based approach, Saiyed et al145 immobilized ALP onto magnetic nanoparticles.…”

Section: Deposition Of Biomoleculesmentioning

confidence: 99%

Wet‐Chemical Deposition of Functional Coatings for Bone Implantology

Nijhuis

Leeuwenburgh

Jansen

2010

Macromolecular Bioscience

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Since the early 1980s, research on the modification of bone implant surfaces by applying coatings has mainly focused on the application of inorganic calcium phosphate (CaP) coatings using physical deposition techniques. Organic components of the extracellular bone matrix, on the other hand, play an essential role in the process of bone healing, but cannot be applied using these physical techniques. Therefore, a recent trend in biomaterials research involves development of novel wet-chemical deposition techniques for both inorganic and organic coatings. This study reviews the major wet-chemical coating techniques that are used for the deposition of inorganic CaP coatings and organic biomolecules coatings.

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Section: Deposition Of Biomoleculesmentioning

confidence: 99%

Wet‐Chemical Deposition of Functional Coatings for Bone Implantology

Nijhuis

Leeuwenburgh

Jansen

2010

Macromolecular Bioscience

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“…For this study, a plastic open-tubular reactor with chemically immobilized urease has been applied. According to immobilization protocol reported elsewhere [ 39 ], the inner walls of PVC tubing have been coated with carboxylated PVC and the functional groups have been applied for covalent binding of urease molecules using a one-step carbodiimide method. The dimensions of bioreactor (90 μL of inner volume of 2.0-cm-long tubing) mounted between LEDs define both the internal volume of the resulting flow cell and the optical path length for photometric measurement.…”

Section: Resultsmentioning

confidence: 99%

“…KH-95871-30) purchased from Cole-Palmer (USA). As proposed elsewhere [ 39 ], the tubing was coated with PVC–COOH by flowing through its solution in THF (60 mg/mL) and evaporation of solvent residues at room temperature. For enzyme immobilization, the reactor was filled with a water solution of urease (20 mg/mL) containing EDAC (10 mg/mL) and left at room temperature overnight.…”

Section: Methodsmentioning

confidence: 99%

Towards optoelectronic urea biosensors

2015

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Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development of optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, immobilized in the form of an open-tubular microbioreactor or biosensing membrane that has been integrated with two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both enzyme-based biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, and well-reproducible urea detection in the millimolar range of concentrations. Potential analytical applications of the developed urea bioPEDDs have been announced. Both presented constructions will be easily adapted for the development of other optoelectronic biosensors exploring various enzyme-based schemes of biodetection.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-014-8434-z) contains supplementary material, which is available to authorized users.

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“…Polycarbonate could be the material of choice for such implementations not only for its malleability and durability, but also for its efficiency in coating with proteins and the resulting large conversion rates in the reactors. For example, a tubular reactor with ALP immobilized using EDC on the inner wall of 1/16-inch poly(vinyl chloride) (PVC) tubing 26 offered smaller throughput than the reactors presented here, while it used ca. 20-fold larger amount of the enzyme in the immobilization procedure and the enzyme presented ca.…”

Section: Discussionmentioning

confidence: 96%

Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

2012

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This paper examines a set of techniques for the immobilization of enzymes on the surface of microchannels fabricated in polycarbonate (PC). Our experiments identify the method that uses combined physico-chemical immobilization on a layer of polyethyleneimine (PEI) as a reproducible vista for the robust immobilization of proteins. As an example, we demonstrate the fabrication, throughput and stability of an open-tubular reactor draped with alkaline phosphatase (ALP, EC 3.1.3.1) as a model enzyme. As PC is suitable for industrial applications the method could potentially be used to immobilize proteins in numbered-up implementations.

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Poly(vinyl chloride) tubing with covalently bound alkaline phosphatase and alternative approach for investigations of open-tubular bioreactors

Cited by 16 publications

References 13 publications

Wet‐Chemical Deposition of Functional Coatings for Bone Implantology

Wet‐Chemical Deposition of Functional Coatings for Bone Implantology

Towards optoelectronic urea biosensors

Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

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