Poly(vinylferrocene-co-2-hydroxyethyl methacrylate) mediator as immobilized enzyme membrane for the fabrication of amperometric glucose sensor

Amperometric Enzyme-based Biosensors for Lowering the Interferences

“…Besides, the other performances including linear range, response time and limit of detection were 1.1-15 mM (human range included), 95-105 s and 1.1 mM, respectively. Comparing to the previous works of co-immobilization of GOD and mediators, the sensitivity is better than 0.79 (Himuro et al, 2009), 0.111 (Crespilho et al, 2008) and 1.86 (Qiu et al, 2007) mA M -1 cm -2 In the first work, the GOD was linked on the mediator-based copolymer backbone, poly(vinylferrocene-co-2-hydroxyethyl methacrylate), and polyamidoamine particle linked by GOD was co-immobilized with the gold nanoparticles modified by cobalt hexacyanoferrate (mediator) on conducting glass in the second work. In the last work, the GOD and ferrocene monocarboxylic acid-modified Fe 3 O 4 nanoparticles were both entrapped on carbon paste electrode.…”

“…In the literatures reported, the covalent method is a more effective way to stabilize the mediators on the electrode. For example, the mediators were linked on the MWCNTs (Qiu et al, 2009), the polymer matrix (Himuro et al, 2009) or even the enzyme directly (Wu et al, 2008). Moreover, the mediator was linked with the electrode and the redox center of enzyme for increasing the efficiency of electron transfer from enzymes to the external circuit through mediators (Zayats et al, 2008).…”

Highly Sensitive Carbon Nanotube-Based Sensing for Lactate and Glucose Monitoring in Cell Culture

“…These results also validate the phenomenon reported from Carrara et al [6] regarding the inverse linear correlation between sensitivity and detection limit when using nanomaterials for biosensing. Comparing the obtained values in this work with nonnanostructured biosensors [27], [28], an improvement of performance with respect to the case of mediated electron-transfer is demonstrated. The advantages brought from nanomaterials are really evident, especially in ranges of concentration which are of interest for a large pool of applications, and they make carbon nanotubes the key-element for the future of nanobiosensing.…”

Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

“…An attractive approach to tackle this challenge is to use a polymeric mediator which has mediator moieties chemically attached to polymer chains. Because of its large molecular size, the polymeric mediator can be co-immobilized with enzyme at electrode by various means, including surface grafting, [31][32][33][34] layer-by-layer surface adsorption, [35][36][37] retention behind semi-permeable dialysis membranes, [38][39][40] physical entrapment [41][42][43] or cross-link in hydrogels, [44][45][46][47] entrapment in electropolymerized [48,49] or chemically formed layers [50] or in inorganic layers, [51,52] and blend in carbon pastes. [53] At LifeScan Scotland Limited, we have synthesized a ferrocene polymeric mediator which is a copolymer of vinylferrocene, acrylamide and 2-(diethylamino)ethyl methacrylate.…”