Poly(Vinylidene Fluoride-Trifluorethylene)/barium titanate membrane promotes de novo bone formation and may modulate gene expression in osteoporotic rat model

Abstract

Osteoporosis is a chronic disease that impairs proper bone remodeling. Guided bone regeneration is a surgical technique that improves bone defect in a particular region through new bone formation, using barrier materials (e.g. membranes) to protect the space adjacent to the bone defect. The polytetrafluorethylene membrane is widely used in guided bone regeneration, however, new membranes are being investigated. The purpose of this study was to evaluate the effect of P(VDFTrFE)/BT [poly(vinylidene fluoride-trifluoroethylene)/barium titanate] membrane on in vivo bone formation. Twenty-three Wistar rats were submitted to bilateral ovariectomy. Five animals were subjected to sham surgery. After 150 days, bone defects were created and filled with P(VDF-TrFE)/BT membrane or PTFE membrane (except for the sham and OVX groups). After 4 weeks, the animals were euthanized and calvaria samples were subjected to histomorphometric and computed microtomography analysis (microCT), besides real time polymerase chain reaction (real time PCR) to evaluate gene expression. The histomorphometric analysis showed that the animals that received the P(VDF-TrFE)/BT membrane presented morphometric parameters similar or even better compared to the animals that received the PTFE membrane. The comparison between groups showed that gene expression of RUNX2, BSP, OPN, OSX and RANKL were lower on P(VDF-TrFE)/BT membrane; the gene expression of ALP, OC, RANK and CTSK were similar and the gene expression of OPG, CALCR and MMP9 were higher when compared to PTFE. The results showed that the P(VDF-TrFE)/BT membrane favors bone formation, and therefore, may be considered a promising biomaterial to support bone repair in a situation of osteoporosis.

Influence of Green Tea Extract with Different Concentrations of Epigallocatechin Gallate on Calvaria Bone Repair of Ovariectomized Rats

“…Histomorphometric results revealed that the mean values of bone neoformation between sham and ovariectomized groups were similar, without statistical significance. These data are in agreement with previous results obtained by our group research (Scalize et al, 2016), despite the high mean values obtained for bone Int. J.…”

Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic bone repair

“…In this study, we selected the PVDF-TrFE/BT membrane due to its good in vitro biocompatibility and the capacity to induce bone formation in both normal and osteoporotic rats and decrease bone resorption by inhibiting osteoclastogenesis. 8,29,30,31,32,33 In addition, in a previous study, the PVDF-TrFE/BT membrane was successfully combined with osteoblast injections to increase the amount of bone formation in the calvarial defects of normal rats. 11 For bone regeneration based on the use of cells, several tissues have been investigated as cell source; however, the bone marrow still remains the most common source, at least in part, due to its ease of accessibility and multilineage differentiation potential.…”

“…The PVDF-TrFE/BT membrane was used as it can promote bone formation in both normal and osteoporotic rats. 8,11 The defect size was selected based on previous studies where no significant bone formation was observed even 12 weeks post-surgery. 15,16 Two weeks postimplantation, the animals were randomly distributed to receive local injections of 50 µl of PBS (Gibco-Invitrogen) containing 5 × 10 6 MSCs collected from healthy rats (n = 5) or 50 µl of PBS without MSCs (Control, n = 5).…”

“…6,7 Recent research has demonstrated promising results using GBR in terms of bone repair of osteoporotic rat calvarial defects, wherein there was induction of more bone formation than that in untreated defects. 8 Although there was increment in bone repair induced by the composite poly(vinylidene-trifluoroethylene)/ barium titanate (PVDF-TrFE/BT) membrane under osteoporotic condition, only small amount of bone formation was observed. This could be, at least in part, due to a decreased migration of mesenchymal stem cells (MSCs) to the defects induced by osteoporosis as it is well known that MSC migration and invasion of injured areas are critical to the process of bone healing.…”

Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation

“…Circular bone defects of 5 mm in the calvaria in adult rats were performed in this study as reports show that such defects provide an adequate model for evaluating bone neoformation in the craniofacial region . The experimental period of 30 days in this study was chosen in line with other reports . During this period a layer of osteoblasts on neoformed bone from irradiated animals was observed, while in the irradiated animals only non‐calcified connective tissue was present.…”