Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia

Aly, Magda M.; Jaar, Tahani Ali Mohamed; Bokhari, Fardus M.

doi:10.9790/3008-1201015361

Cited by 3 publications

(4 citation statements)

References 26 publications

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“…Similarly, a previous study reported that A. fumigatus was able to grow actively on natural and synthetic polyester polymers [30]. Furthermore, soil isolates of A. fumigatus were able to degrade poly-β-hydroxybutyrate acid (PHB) biopolymers [31].…”

Section: Discussionmentioning

confidence: 93%

“…Scherer found that 30 • C is the optimum temperature for the growth of A. fumigatus [30]. While Aly and coworkers [31] reported that the maximum PHB depolymerase production by A. fumigatus was at pH 5 and 30 • C. In addition, Jung and his colleagues showed that A. fumigatus strain 76T-3 grew better at a temperature of 40 • C [23]. While Al Hosni and coworkers found that the most suitable temperature for A. fumigatus to biodegrade polycaprolactone (PCL) is 25-37 • C [40].…”

Section: Discussionmentioning

confidence: 99%

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Optimizing Eco-Friendly Degradation of Polyvinyl Chloride (PVC) Plastic Using Environmental Strains of Malassezia Species and Aspergillus fumigatus

El-Dash,

Yousef,

Aboelazm

et al. 2023

IJMS

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Worldwide, huge amounts of plastics are being introduced into the ecosystem, causing environmental pollution. Generally, plastic biodegradation in the ecosystem takes hundreds of years. Hence, the isolation of plastic-biodegrading microorganisms and finding optimum conditions for their action is crucial. The aim of the current study is to isolate plastic-biodegrading fungi and explore optimum conditions for their action. Soil samples were gathered from landfill sites; 18 isolates were able to grow on SDA. Only 10 isolates were able to the degrade polyvinyl chloride (PVC) polymer. Four isolates displayed promising depolymerase activity. Molecular identification revealed that three isolates belong to genus Aspergillus, and one isolate was Malassezia sp. Three isolates showed superior PVC-biodegrading activity (Aspergillus-2, Aspergillus-3 and Malassezia) using weight reduction analysis and SEM. Two Aspergillus strains and Malassezia showed optimum growth at 40 °C, while the last strain grew better at 30 °C. Two Aspergillus isolates grew better at pH 8–9, and the other two isolates grow better at pH 4. Maximal depolymerase activity was monitored at 50 °C, and at slightly acidic pH in most isolates, FeCl3 significantly enhanced depolymerase activity in two Aspergillus isolates. In conclusion, the isolated fungi have promising potential to degrade PVC and can contribute to the reduction of environmental pollution in eco-friendly way.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Optimizing Eco-Friendly Degradation of Polyvinyl Chloride (PVC) Plastic Using Environmental Strains of Malassezia Species and Aspergillus fumigatus

El-Dash,

Yousef,

Aboelazm

et al. 2023

IJMS

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“…For fungi, growth on agar medium is generally more comfortable than in broth medium (Aly et al, 2017). Thus, no further assessment was conducted for isolates that showed weakly activity on solid agar medium containing PHB as a carbon source.…”

Section: Discussionmentioning

confidence: 99%

Optimisation of growth conditions for maximum degradation of poly βhydroxyl butrate by two soil fungi

Aly¹,

Bokhari²,

Albureikan³

et al. 2020

ijrps

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Poly β- hydroxyl butrate (PHB) is a polymer produced by bacteria and can be used safely in different modern application to replace biobased plastic which has adverse effects on the environments. This study is aimed to isolate some fungi for PHB degradation and optimise the growth conditions for maximum degradation. Contaminated soil samples were collected from the industrial area of Jeddah and used for fungal isolations. All fungal isolates were screened for PHB biodegradation on solid agar medium. Out of 20 isolates, 2 isolates were the most active in PHB degradation. They were identified as Trichoderma asperellum NM 6, and Aspergillus fumigates NM10 using morphological and molecular methods. The effects of some growth factors on growth and PHB degradation by the two isolated fungi were determined. Growth was measured by either CPV (ml) or dry weight (g/l) while PHB degradation was detected by depolymerase assay (U/ml). Presence of yeast extract (2.5 g/l) and glucose (1 g/l) enhanced both growth and PHB degradation by the two isolates. Similarly, adjusting medium pH at 7-7.5 and incubation at 25°C after for five days led to maximum growth and PHB degradation. The presence of PHB in growth medium enhanced both growth and PHB degradation and the maximum growth (CPV ml or dry weight (g/l) were in medium containing 0.5 g/l of PHB for both tested fungi. The maximum growth, measured by either CPV (ml) or dry weight (g/l), was in the medium that was inoculated with 10x106 spore/ml for the two tested fungi. In conclusion, PHB degradation by the two tested fungi was similar. It was affected by the same physical and biochemical factors and optimisation of these conditions enhanced both growth and degradation process by the two tested fungi.

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“…Aspergillus has been known as one of the most predominant genera of lipase-producing and PHB-degrading molds (Matavulj and Molitoris, 1992). Some studies also reported that these molds had the ability to degrade PHB (Kumaravel et al, 2010;Lodhi, et al, 2011;Merugu, 2012;Aburas, 2016;Aly et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

Yanti

Sriwahyuni

Rayani

et al. 2019

Preprint

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The present study was investigated the potential of lipolytic fungi (molds) isolated from landfill soil in degrading Poly-β-hydroxybutyrate (PHB). Screening of PHB-degrading lipolytic molds was done in two stages, such as screening of lipolytic molds which was identified by the formation of orange fluorescent halos around the colony on rhodamine B agar medium and the degradation PHB ability test was identified by the formation of clear zone around colony on PHB emulsion medium. Characterization of isolates was done based on phenotypic characters and the identification was done by numerical-phenetic analysis. Three lipolytic mold isolates that have ability in degrading PHB bioplastic i.e isolate KC1, KE1 and KE6. These molds have asexual spore form conidia, foot cell, septate hyphae, unbranched conidiophore, and spore mass located at the apex of phialid. The identification results showed that isolate KC1 is identic to Aspergillus terreus, KE1 is identic to Aspergillus niger and KE6 is identic to Aspergillus fumigatus.

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Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia

Abstract: Poly-β-hydroxybutyrate

Cited by 3 publications

References 26 publications

Optimizing Eco-Friendly Degradation of Polyvinyl Chloride (PVC) Plastic Using Environmental Strains of Malassezia Species and Aspergillus fumigatus

Optimizing Eco-Friendly Degradation of Polyvinyl Chloride (PVC) Plastic Using Environmental Strains of Malassezia Species and Aspergillus fumigatus

Optimisation of growth conditions for maximum degradation of poly βhydroxyl butrate by two soil fungi

The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

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