PolyA-tailed and fluorophore-labeled aptamer-gold nanoparticle conjugate for fluorescence turn-on bioassay using iodide-induced ligand displacement

Li, Wei; Dong, Yifan; Wang, Xi; Li, Hui; Xu, Danke

doi:10.1016/j.bios.2014.10.047

Cited by 29 publications

(16 citation statements)

References 42 publications

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“…The preparation of the conjugates is rather time-consuming (1-2 days) and inevitably costly [24]. Since vulnerable RNA chains may be degraded during the preparation process, few reports have described the use of RNA-AuNP conjugates in biomedical applications.…”

Section: Resultsmentioning

confidence: 99%

A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk

et al. 2016

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To date, there are few reports regarding the development of RNA aptamer-based biosensors for the detection of small molecules. The possible reason is attributed to the weak nuclease resistance of RNA in biological environments. In this study, we have developed an RNA aptamer-based gold nanoparticle (AuNP) sensor for fluorometric detection of Neomycin B in milk. This aptasensor depends on the selfassembly of the RNA aptamer/Neomycin B complex and fluorescence quenching by AuNPs. This biosensor exhibited a low detection limit of 0.01 μM, with a linear dynamic range from 0.1 to 10 μM in milk, and a good selectivity toward Neomycin B. Specifically, because of the shorter RNA fragments and the nuclease inhibition ability of the RNA-modified AuNPs, the RNA sequences remained stable during the experiments. This work will serve as an example for the development of novel biosensors based on RNA aptamers.

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Section: Resultsmentioning

confidence: 99%

A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk

et al. 2016

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“…More interestingly, AuNP:poly-A10-ASSVd LAMP barcodes was accompanied by several remarkable advantages compared to the conjugation methods, which is more stable when adjusting the length of poly-A for a long storage time period. 36−39 A high density of probe attachment was achieved drastically at low pH in a few minutes, 40,41 and poly-A-based AuNP conjugate adopts an upright conformation favorable for hybridization. 42 ■ EXPERIMENTAL SECTION Total RNA Extraction from Apple Leaves and Sequencing Analysis.…”

Section: ■ Resultsmentioning

confidence: 99%

Poly-adenine-Coupled LAMP Barcoding to Detect Apple Scar Skin Viroid

et al. 2018

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Apple Scar Skin Viroid (ASSVd), a nonprotein coding, circular RNA pathogen is relatively difficult to detect by immunoassay. We report here a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to improve selectivity for diagnostic use in detecting ASSVd in plants. ASSVd RT-LAMP was accelerated using loop primers and was found to be highly sensitive with a detection limit of 10 copies of cDNA-ASSVd within 30 min. Real-time LAMP and melting curve analysis could differentiate between the true-positive LAMP amplicons and false-positive nonspecific primer amplification products. The optimized RT-LAMP was then followed by the addition of nonthiolated AuNP:poly-adenine (A10)-ASSVd LAMP barcodes, showing a high authentication capacity with colorimetric changes. This type of barcoding assay is a potential alternative for rapid and multiple viroid diagnosis, providing for visible sensing in the field that can be applied to viroid-free planting.

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“…In our previous work, many assays in use of DNA-functionalized metal nanoparticles have been developed for protein detection [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

PolyA-tailed and fluorophore-labeled aptamer-gold nanoparticle conjugate for fluorescence turn-on bioassay using iodide-induced ligand displacement

Cited by 29 publications

References 42 publications

A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk

A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk

Poly-adenine-Coupled LAMP Barcoding to Detect Apple Scar Skin Viroid

A highly sensitive fluorescence turn-on platform with silver nanoparticles aptasening for human platelet-derived growth factor-BB

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