Polyacrylamide-gel disc electrophoresis of native cerebrospinal fluid proteins with special reference to immunoglobulins and some clinical applications

Takeoka, Tsuneyuki; Gotoh, Fumio; Furumi, Koichi; Mori, Kosuke

doi:10.1016/0022-510x(76)90172-6

Cited by 14 publications

(7 citation statements)

References 23 publications

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“…3 (7 fraction). This alteration of CSF protein patterns due to the BCB damage is not specific to MS, and has been observed in other neurological diseases (Takeoka et al, 1976(Takeoka et al, , 1980. The percent of CSF prealbumin had been found to be decreased in the acute phase of definite MS, with a concomitant increase of total protein concentration (Lamoureux et al, 1975).…”

Section: Discussionmentioning

confidence: 55%

“…Normal reference specimens of CSF reported previously (Takeoka et al, 1976) were obtained from 27 subjects without apparent neurological diseases, at the time of spinal anesthesia for minor surgery.…”

Section: Csf Specimensmentioning

confidence: 99%

“…The total protein content of the CSF was determined by spectrophotometric (Hitachi model 200-10, Tokyo, Japan) turbidimetry with 3% sulphosalicylic acid (Meulemans, 1960), using normal human control serum (Hyland Lab., CA, USA) as a standard. The upper limit of normal value was taken as 45 mg/dl (Takeoka et al, 1976).…”

Section: Determination Of Protein Concentrationmentioning

confidence: 99%

“…(Takeoka et al, 1980). Immunoglobulins were fractionated in the G zone (Takeoka et al, 1976), as demonstrated by immunofixation in the left-hand gel of the figure. The polymers of the Hp 2-1 or 2-2 bands were not observed in a disc electrophoretogram, or were hardly detectable in the G zone by immunofixation, even though the serum Hp phenotype of this case was type 2-2.…”

Section: Immunofixationmentioning

confidence: 99%

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Impairment of Blood‐Cerebrospinal Fluid Barrier in Multiple Sclerosis

Takeoka

Shinohara

Furumi

et al. 1983

Journal of Neurochemistry

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The blood-CSF barrier (BCB) function in active multiple sclerosis (MS) was studied by means of CSF proteins analysis using disc electrophoresis and immunofixation. Forty-five CSF samples were obtained by repeat lumbar punctures at various intervals, from four autopsy-proven cases and three male and nine female patients with clinically definite MS. When total protein content was increased, the percentages of prealbumin and tau fraction were decreased significantly in association with the presence of haptoglobin (Hp) polymers in nearly all the samples, as a result of increased permeability of the BCB. Even when the total protein content was normal, Hp polymers were detected in 56% of the samples, and the tau fraction tended to be decreased. Monoclonal immunoglobulin and Hp polymers were both recognized in some cases. The results suggested a more frequent occurrence of BCB impairment in MS than had formerly been revealed by CSF albumin analysis, and accorded with the recent reports of contrast-enhancing lesions of MS brain in computerized tomography.

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Section: Discussionmentioning

confidence: 55%

Section: Csf Specimensmentioning

confidence: 99%

Section: Determination Of Protein Concentrationmentioning

confidence: 99%

Section: Immunofixationmentioning

confidence: 99%

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Impairment of Blood‐Cerebrospinal Fluid Barrier in Multiple Sclerosis

Takeoka

Shinohara

Furumi

et al. 1983

Journal of Neurochemistry

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“…Little has been reported about the concentration of CSF-TP in Asian populations. To our knowledge, only two studies have reported on CSF-TP in Japanese patients, but these were presented over 30 years ago [11,12]. Since the CSF measuring methods and the accuracy of testing have been greatly improved in the past decades, it is crucial to review the CSF-TP reference interval for various populations [2,11,12].…”

Section: Introductionmentioning

confidence: 99%

Cerebrospinal Fluid Protein Concentration in Healthy Older Japanese Volunteers

Yoshihara

Zaitsu

Ito

et al. 2021

IJERPH

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The concentration of cerebrospinal fluid total protein (CSF-TP) is important for the diagnosis of neurological emergencies. Recently, some Western studies have shown that the current upper reference limit of CSF-TP is quite low for older patients. However, little is reported about the concentration of CSF-TP in the older Asian population. In this study, we retrospectively analyzed the CSF-TP concentrations in healthy older Japanese volunteers. CSF samples in 69 healthy Japanese volunteers (age range: 55–73 years) were collected by lumbar puncture, and the data of CSF were retrospectively analyzed. The mean (standard deviation) CSF-TP was 41.7 (12.3) mg/dL. The older group (≥65 years old) had higher CSF-TP concentration than the younger group (55–64 years old). The 2.5th percentile and 97.5th percentile of CSF-TP were estimated as 22.5 and 73.2 mg/dL, respectively, which were higher than the current reference range in Japan (10–40 mg/dL). Conclusions: The reference interval of CSF-TP in the older population should be reconsidered for the precise diagnosis of neurological emergencies.

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Human Cerebrospinal Fluid Immunology

Trotter¹,

Rust²

1989

The Cerebrospinal Fluid

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Polyacrylamide-gel disc electrophoresis of native cerebrospinal fluid proteins with special reference to immunoglobulins and some clinical applications

Cited by 14 publications

References 23 publications

Impairment of Blood‐Cerebrospinal Fluid Barrier in Multiple Sclerosis

Impairment of Blood‐Cerebrospinal Fluid Barrier in Multiple Sclerosis

Cerebrospinal Fluid Protein Concentration in Healthy Older Japanese Volunteers

Human Cerebrospinal Fluid Immunology

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