Polyacrylamide Gel Electrophoresis of Whole-Cell Proteins of Porcine Strains of Haemophilus

Nicolet, Jacques; Paroz, P; Krawinkler, M.

doi:10.1099/00207713-30-1-69

Cited by 28 publications

(19 citation statements)

References 34 publications

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“…with two 100% S subgroups; however. to what extent these subgroups could be related to type I and type II biovars, as determined from comparative protein profiles (Nicolet and Krawinkler 1981;Nicolet et al 1980), remains unknown. Also uncertain is the taxonomic position of Haemophilus sp.…”

Section: Nztnzerical Taxonomic Analysismentioning

confidence: 99%

Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications

O'Reilly¹,

Rosendal²,

Niven³

1984

Can. J. Microbiol.

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Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both strips allowed adequate differentiation of closely related organisms. Numerical taxonomic analysis of the data demonstrated that the majority of the haemophili and actinobacilli studied could be placed in one of four major clusters; these clusters contained, respectively, the H. pleuropneumoniae--A. pleuropneumoniae strains, the H. parasuis strains, strains belonging to Haemophilus taxon "minor group," and strains belonging to an unusual group of mannitol-positive, urease-negative haemophili. A representative of Haemophilus species taxon C and an unusual Actinobacillus isolate appeared to be comparatively unrelated to organisms in the four major clusters. Although it may, on occasion, be difficult to place an unusual isolate in any one particular group, owing to the uncertain taxonomy of some of these organisms, it is concluded that API test strips can serve as useful tools for the characterization and differentiation of porcine haemophili and actinobacilli.

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Section: Nztnzerical Taxonomic Analysismentioning

confidence: 99%

Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications

O'Reilly¹,

Rosendal²,

Niven³

1984

Can. J. Microbiol.

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“…Two different OMP profiles, biotype I and biotype II, were identified in H. parasuis strains using SDS-PAGE (Nicolet et al, 1980;Morozumi and Nicolet, 1986a;Ruiz et al, 2001). Isolates from nasal mucosa of healthy pigs represent biotype I with proteins of molecular weight of about 68 kDa and between 23-40 kDa.…”

Section: Virulence and Virulence Factorsmentioning

confidence: 99%

Haemophilus parasuis and Glässer's disease in pigs: a review

Nedbalcová¹,

Satran²,

Jaglič³

et al. 2006

Vet. Med.

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ABSTRACT:Haemophilus parasuis is a common epiphyte of the upper respiratory tract of pigs. The factors of H. parasuis pathogenicity that enable some strains to be virulent and consequently cause a clinical disease have not been established yet. Fifteen serovars of H. parasuis have been described at present. Individual serovars differ in virulence, and considerable differences in virulence also exist within each serovar. Virulent strains can particularly participate as microorganisms secondary to pneumonia, cause septicaemia without polyserositis or Glässer's disease characterized by polyserositis, pericarditis, arthritis and meningitis. Clinical symptoms of this disease are highly variable. Therefore, culture detection of causative agent, particularly from the brain, joints and polyserositis is an essential diagnostic tool. The disease caused by H. parasuis can be treated with antibiotics; however, oral or parenteral administration of very high doses of antibiotics is necessary. The level of animal hygiene and animal husbandry are important factors for prevention of this disease. Commercial or autogenous vaccines can be used in the immunoprophylaxis of pre-parturient sows and their progeny after weaning. For the production of autogenous vaccines, it is most effective to use isolates from animals with lesions present in CNS. Isolates recovered from arthritic and systemic sites of infection are less suitable and isolates recovered from lungs are not suitable at all because of their heterogeneity.

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“…parasuis show almost uniform biochemical properties (3), it is possible to differentiate them by polyacrylamide gel electrophoresis (PAGE) of sodium dodecyl sulfate-solubilized cells into two types based on different peptide patterns, PAGE types I and I1 (7). The PAGE type I1 strains are always associated with pathologic conditions, particularly with GlAsser's syndrome.…”

mentioning

confidence: 99%

Deoxyribonucleic Acid Relatedness among Strains of Haemophilus parasuis and Other Haemophilus spp. of Swine Origin

Morozumi¹,

Pauli

Braun

et al. 1986

International Journal of Systematic Bacteriology

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The levels of cross-hybridization between deoxyribonucleic acids from Haemophilus parasuis, Haemophilus pleuropneumoniae, and unnamed Haemophilus taxon C were less than 15 % . The levels of deoxyribonucleic acid complementarity between H . parasuis strains belonging to serovars 1 to 4 and polyacrylamide gel electrophoresis type I and I1 strains were very high (86 f 7%), but the levels of complementarity between strain Nagasaki (serovar 5, polyacrylamide gel electrophoresis type 11) and the remaining strains of H . parasuis were rather low (64 * 5%). Our results indicate that, with the exception of serovar 5, the species H . parusuis comprises a rather homogeneous, genetically closely related group of bacteria. A further division of H . parasuis strains into subspecies according to their polyacrylamide gel electrophoresis peptide profiles is not justified on the basis of deoxyribonucleic acid sequence homologies. However, the taxonomic position of serovar 5 needs further investigation.Haemophilus parasuis (2) is a well-known species in veterinary bacteriology. This organism is a widespread and common inhabitant of the nasal cavities of pigs and is also involved in porcine respiratory diseases as a secondary invader. Furthermore, it is recognized as the agent of Glasser's syndrome (fibrinous polyserositis, meningitis, polyarthritis, or septicemia) (8).Although the strains of H . parasuis show almost uniform biochemical properties (3), it is possible to differentiate them by polyacrylamide gel electrophoresis (PAGE) of sodium dodecyl sulfate-solubilized cells into two types based on different peptide patterns, PAGE types I and I1 (7). The PAGE type I1 strains are always associated with pathologic conditions, particularly with GlAsser's syndrome. In addition, recent, more detailed studies have shown that some structural heterogeneity (capsulation, susceptibility to acriflavine, etc.) can occur, which allows the strains (6) to be classified into at least five serovars (unpublished data).Consequently, we wanted to determine whether H . parasuis strains could also be separated genetically and whether the deoxyribonucleic acid (DNA) patterns could be correlated with PAGE types and serovars. There is little information concerning the genetic relationships among H . parasuis strains, except that DNA base compositions have been determined for a few strains (3).In this study we examined the DNA relationships amorig strains belonging to different serovars and having different PAGE profiles and compared the levels of hybridization with other species isolated from pigs, including Haemophilus (Actinobacillus) pleuropneumoniae biovar 1 (10) and Haemophilus sp. taxon C (3). MATERIALS AND METHODS Bacterial strains.The following H . parasuis strains were used in this study: strains NCTC 7440 (National Collection of Type Cultures, London, England) and 4 (Japan), representing serovar 1; strain SW140 (Japan), representing serovar 2; strain SW114 (Japan), representing serovar 3; strain SW124 (Japan), representing serovar 4; and strain ...

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Polyacrylamide Gel Electrophoresis of Whole-Cell Proteins of Porcine Strains of Haemophilus

Cited by 28 publications

References 34 publications

Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications

Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications

Haemophilus parasuis and Glässer's disease in pigs: a review

Deoxyribonucleic Acid Relatedness among Strains of Haemophilus parasuis and Other Haemophilus spp. of Swine Origin

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