Polyacrylamide Gel Electrophoretic Study on the Effect of Estrogen on Human Thyroxine-binding Prealbumin

Sato, Toshiro; Saito, Shintaro; Inagaki, Kiyoshi; Tayama, Sumio; Torikai, T

doi:10.1620/tjem.93.339

Cited by 18 publications

(6 citation statements)

References 29 publications

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“…When palmitic acid was added to serum in vitro, however, no increase in binding capacity of TBPA was observed. Such so-called see-saw phenomena in the binding capacity of TBG and TBPA have already been reported with regard to estrogen (Torikai 1967, Sakurada et al 1967) and steroid (Werner and Platman 1965) administration. It is interesting that the binding capacity of TBP induced by exogenous agents shows a see-saw like change, unlike the decrease of binding capacity of both TBG and TBPA in hyperthyroid patients (Sakurada et al 1968).…”

Section: ) Results Of In Vitro Observationmentioning

confidence: 61%

“…T4 binding capacity of TBG was measured according to the method of Tanaka and Starr (1959). Distribution of 131I-T4 to each TBP fraction, binding capacity and concentration of TBPA were measured by the method of polyacrylamide gel electrophoresis described in our previous report (Sakurada et al 1967). Blood sugar was determined by the autoanalyzer.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Heparinoid on Free Thyroxine Concentration in Human Serum

Sato

Tayama

Saito

et al. 1973

Tohoku J. Exp. Med.

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Sodium salt of dextran sulfate ester (MDS-Kowa, Kowa Co., Japan) was administered intravenously to 4 euthyroid subjects, 7 patients with hyperthyroidism and 5 patients with hypothyroidism. Serum FFA rose to the level 4-6 times higher than the preinjection level 15 min after the intravenous injection. At the same time, elutable fraction (EF) of T4, free T4, and binding capacity and concentration of TBPA increased, while binding capaty of TBG decreased. Addi tion of dextran sulfate to serum in vitro caused no change in binding capacity of TBG and EF of T,,. Addition of palmitic acid to serum in vitro with increas ing concentration resulted in a gradual decrease in the distribution of 131I-T4 to TBG and binding capacity of TBG, while EF of T4 gradually increased. Intravenous injection of dextran sulfate ester following the prior injection of protamine sulfate in dog caused a mild rise in serum FFA, but failed to increase the EF of T4. When reverse-flow paper electrophoresis was carried out after addition of 14C-palmitic acid to sera of normal and TBG-deficient subjects, it was confirmed that palmitic acid bound to albumin and TBG fraction.These results suggest that dextran sulfate ester raises serum FFA first, then the increased FFA combines with TBP at least with TBG, thus competing for binding sites with T4, and released T4 in turn increases free T4 level in the serum.heparinoid; free thyroxine; thyroxine-binding globulin Hollander et al. (1967) first reported that free fatty acids (FFA) and serum per cent free thyroxine (T4) increased by the intravenous injection of heparin. Braverman et al. (1969), on the other hand, reported that fluctuation of the con centration of FFA within the physiological range has little effect on free T4 in serum. The competition of FFA with T4 for one or other of T ,-binding globulin. (TBG), T4-binding prealbumin (TBPA) and albumin was supposed to be the cause of increase of free T4 through the rise of FFA.In the present report, sodium salt of dextran sulfate ester, a heparinoid which has intense lipemia-clearing activity,, was used to study the effect of FFA upon T4-binding protein (TBP) and free T4.

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Section: ) Results Of In Vitro Observationmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Effect of Heparinoid on Free Thyroxine Concentration in Human Serum

Sato

Tayama

Saito

et al. 1973

Tohoku J. Exp. Med.

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“…The patient showed, as in hypothyroidism (Sakurada et al 1967) elevated binding capacity and concentration of TBPA which does not bind T3 (Tata et al 1961). These findings were consistent with the report of Oppenheimer et al (1965), and this may suggest a possible involvement of T4 in the metabolism of TBPA.…”

mentioning

confidence: 90%

“…Serum BEI (Kontaxis and Pickering 1958) was low , but resin sponge uptake of 131I-T3 (Triosorb Test, Abbott Co.) was normal . Binding capacity of thyroxine-binding globulin (TBG) (Tanaka and Starr 1959) was within the normal limits, and concentration and binding capacity of thyroxine-binding prealbumin (TBPA) (Sakurada et al 1967) were high. Both elutahle fractions of T4 and T3 ) (per cent free T4 and T3) were very low.…”

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confidence: 99%

A Case of Triiodothyronine-secretor with Hypometabolism

Sato¹,

Demura²,

Onodera³

et al. 1973

Tohoku J. Exp. Med.

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“…was low, but serum free T3 was normal in all four members. When T4 binding capacity of TBPA was measured by the method of the present author (Sakurada et al 1967) (polyacrylanude gel electrophoresis, borate buffer, pH 8.2) and by the T4-kinetics (Table 5): T4 kinetics in Case H.T. was studied by the method of Ingbar and Freinkel (1955).…”

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confidence: 99%

Effect of Estrogen on Serum Total and Free Thyroxine and Triiodothyronine in a Thyroxine-binding Globulin Deficient Family

Sato

Yamaguchi

Yamamoto

et al. 1974

Tohoku J. Exp. Med.

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a family with X-chromosome-linked inheritance of thyroxine (T4)-binding globulin (TBG) deficiency, serum free T4 values were low in three of four members, but free triiodothyronine (T3) values were normal in all four of them. Binding capacities of T4-binding prealbumin (TBPA) were high in three of four members. In the propositus, a 47-year-old male, whose T4-binding capacity of TBG was null, half-time of serum T4 was shortened, turnover rate, distribution space and clearance rate all increased, extrathyroidal organic iodine pool decreased and degradation rate of T4 was unchanged compared with normal values. While half-time of his serum T3 was prolonged, turnover rate, extrathyroidal pool and degradation rate of T3 decreased, and T3 distribution space and clearance rate increased compared with normal values.When 0.24 mg of ethinylestradiol-3-methylester was administered daily by mouth to the propositus for 1 month, halftime of serum T4 was prolonged, total T4 increased and T4-binding capacity of TBPA decreased but free T4, T4-binding capacity of TBG and the degradation rate of T4 were unchanged.Meanwhile half-time of his serum T3 was shortened, total and free T3 levels and degradation rate of T3 increased.T3 radioimmunoassay; estrogen; free T3 and T4; TBG-deficiency The first euthyroid man with deficient TBG was reported by . In the present paper, the metabolism of T4 and T3 and binding capacities of TBG and TBPA and the effect of estrogen on them are studied in a TBG-deficient family.

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Polyacrylamide Gel Electrophoretic Study on the Effect of Estrogen on Human Thyroxine-binding Prealbumin

Cited by 18 publications

References 29 publications

Effect of Heparinoid on Free Thyroxine Concentration in Human Serum

Effect of Heparinoid on Free Thyroxine Concentration in Human Serum

A Case of Triiodothyronine-secretor with Hypometabolism

Effect of Estrogen on Serum Total and Free Thyroxine and Triiodothyronine in a Thyroxine-binding Globulin Deficient Family

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