Polyacrylic polyhydrazides as reagents for detection of glycoproteins

Heimgartner, Urs; Kozulié, Branko; Mosbach, Klaus

doi:10.1016/0003-2697(89)90414-4

Cited by 16 publications

(1 citation statement)

References 32 publications

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“…[2] One group of applications deals with immobilization reactions. Examples include the immunochemical detection of glycosphingoli pids on thin-layer chromatograms, [16,18] the detection of glycoproteins, [16,17] and affinity chromatography. [20,21] Synthetic ONs modified at their 5'-end with an aldehyde group can be easily attached to latex microspheres containing hydrazide moieties.…”

Section: Introductionmentioning

confidence: 99%

A Novel Versatile Phosphoramidite Building Block for the Synthesis of 5'- and 3'-Hydrazide Modified Oligonucleotides

Antsypovich¹,

Kiedrowski²

2005

Nucleosides, Nucleotides & Nucleic Acids

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We introduce a novel versatile phosphoramidite building block for the modification of oligonucleotides (ONs) with acyl hydrazides on the 5'- or 3'-terminus, or both. The reaction of these hydrazide functionalized ONs with 4-methoxyphenylaldehyde is demonstrated for solution derivatization. Hydrazides are considered nowadays as promising reactants, which show enhanced reactivity at neutral and slightly acidic conditions and higher stability of yielding products as compared to the aliphatic amines, which are broadly used for ONs derivatization. Our method to introduce hydrazides into ONs employs a phosphoramidite modifier designed to split, during ammonia or lithium hydroxide treatment, into two hydrazides via beta-elimination of a central bis-2-carbonylethoxysulfone unit. It allows the creation of ONs derivatized with a hydrazide moiety at the 5'-, 3'- and both 5'- and 3'-termini, as well as two different hydrazide containing ONs at the same time, viz. in one sequence on the same solid support In latter case one can, for example, synthesize two hydrazide containing ONs, where one is 5'-modified and second one is 3'-modified.

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Section: Introductionmentioning

confidence: 99%

A Novel Versatile Phosphoramidite Building Block for the Synthesis of 5'- and 3'-Hydrazide Modified Oligonucleotides

Antsypovich¹,

Kiedrowski²

2005

Nucleosides, Nucleotides & Nucleic Acids

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Increased selectivity in the detection of glycoproteins on nitrocellulose membranes by washing with sodium hydroxide solution

Kondo

Harada

Sunada³

et al. 1991

Electrophoresis

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We increased selectivity in the detection of glycoproteins on nitrocellulose membranes by introducing a washing step using sodium hydroxide solution. Glycoproteins on the nitrocellulose membrane were first oxidized by sodium periodate; biotin hydrazide was then coupled to the aldehyde groups generated in the sugar moiety of the glycoproteins. The membrane was washed twice using sodium hydroxide solution, and avidin-horseradish peroxidase was then coupled to the remaining biotin. This system allows the detection of nanograms of glycoproteins on nitrocellulose membranes, and its specificity allows the clear distinction of glycoproteins from the nonglycosylated protein of bovine serum albumin.

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Toxic Extracellular Enzymes

Kalisz¹,

Kalisz²

1992

Plant Toxin Analysis

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Polyacrylic polyhydrazides as reagents for detection of glycoproteins

Cited by 16 publications

References 32 publications

A Novel Versatile Phosphoramidite Building Block for the Synthesis of 5'- and 3'-Hydrazide Modified Oligonucleotides

A Novel Versatile Phosphoramidite Building Block for the Synthesis of 5'- and 3'-Hydrazide Modified Oligonucleotides

Increased selectivity in the detection of glycoproteins on nitrocellulose membranes by washing with sodium hydroxide solution

Toxic Extracellular Enzymes

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