PolyAdenine cryogels for fast and effective RNA purification

Köse, Kazım; Erol, Kadir; ÿzgür, ErdoL·an; Uzun, Lokman; Deni̇zli̇, Adil

doi:10.1016/j.colsurfb.2016.07.003

Cited by 32 publications

(19 citation statements)

References 39 publications

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“…In the case of elemental analysis results, the poly(HEMA-GMA)@Nic-Ca(II) cryogels have the N% value corresponding the ligand amount of 32.9 mg/g cryogel (nicotinamide 1.175 mmol/g cryogel). The surface area of cryogels was estimated as 8.736 m 2 /g which quite comparable with the literature (24,25). The Ca(II) amount immobilized on the cryogels was found as 455 mmoles/g.…”

Section: Results and Discussion Characterization Studiessupporting

confidence: 87%

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

Erol¹

2016

Journal of the Turkish Chemical Society, Section A: Chemistry

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Abstract:The separation and purification of an important biomolecule calmoduline is extremely important. The adsorption technique is quite popular, and the cryogels as adsorbents with the macro porous structure and interconnected flow channels are preferred in this field. In this study, the adsorption of calmoduline via Ca(II) immobilized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA), cryogels was studied through changing interaction time, calmoduline initial concentration, and temperature conditions. For the characterization of cryogels, the swelling test, Fourier Transform Infrared (FT-IR) Spectroscopy, Scanning Electron Microscopy (SEM), surface area (BET), elemental analysis and ICP-OES methods were performed. Nicotinamide molecule was used as Ca(II) being the chelating agent and the adsorption capacity of the cryogels was estimated as 1.812 mg calmoduline/g cryogel. The adsorption models of the process were examined via the Langmuir and Freundlich isotherm models.

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Section: Results and Discussion Characterization Studiessupporting

confidence: 87%

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

Erol¹

2016

Journal of the Turkish Chemical Society, Section A: Chemistry

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“…Because of cryogelic polymerization, there were big cavities formed as a result of water removal at the end of the polymerization. The cavities with rough surface (approximately 3.343 m2/g, via Quantachrome AutosorbVR iQ-Chemi, Florida, USA, which was comparable with the literature [26][27][28]) suitable for the adsorption of lead ions could be seen it SEM (Carl Zeiss AG-EVO® 50 Series, Germany) images given in Figure 2. The water uptake amount of cryogels was found (376% on average) using the difference between the weights of dry and water retained cryogel according to the formula given by Equation 1.…”

Section: Synthesis and Characterization Of Poly(2hydroxyethylmethacrysupporting

confidence: 72%

Lead Removal from Industrial Waste

Bilgin.

Yüce

Köse

et al. 2017

Hittite J. Sci. Eng.

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T he increasing number of factories with the heavy metal wastes, especially lead, such as a battery, dye, and fertilizer manufacturing, etc. [1], in consequence of the fast developments of agricultural and industrial fields, threaten the ecological environment and human health. The heavy metal ions cannot be degraded, and resulted in an accumulation in the living organism tissues [2]. The lead limits in drinking water allowed by the World Health Organization is 10 μg/L [3]. Moreover, even very little concentration of lead can cause severe consequences for living things including humans [4]. Because its high affinity to enzymes having thiol (-SH) and phosphate ions (PO 4 3-), ligands and biomolecules inhibiting the haem biosynthesis, Pb 2+ ions are very hazardous for the permeability of cell membranes in kidney, liver, and brain and likewise toxic for nervous and reproduction system, resulting in abortion, dead and neonatal birth, mental retardation [5, 6], etc. Additionally, it may form complexes with the oxogroups of enzymes which are very active in porphyrin metabolism and haemoglobin synthesis [7]. Besides the potential neurological and carcinogenic effects of lead, it may cause cognitive and physical retardation in child development, especially in the interval of 0-7 age [8]. Lead has been classified as a carcinogenic

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“…Cryogel is a macroporous material composed of a cross-linked hydrophilic polymer network with well-controlled macroporosity (with pore size between 1 and 100 μm). 31 − 33 The large pores and interconnected channels of cryogels are the products of cryogelation polymerization. 32 These macroporous structures allow a high flow rate and fast mass transfer, making it possible to realize the direct separation of cells even from complex biological samples without complicated pretreatment processes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Boronic Acid-Functionalized Cryogels Using Modular and Clickable Building Blocks for Bacterial Separation

Hajizadeh

Gong

Lin

et al. 2020

J. Agric. Food Chem.

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Composite cryogels containing boronic acid ligands are synthesized for effective separation and isolation of bacteria. The large and interconnected pores in cryogels enable fast binding and release of microbial cells. To control bacterial binding, an alkyne-tagged boronic acid ligand is conjugated to azide-functionalized cryogel via the Cu(I)-catalyzed azide–alkyne cycloaddition reaction. The boronic acid-functionalized cryogel binds Gram-positive and Gram-negative bacteria through reversible boronate ester bonds, which can be controlled by pH and simple monosaccharides. To increase the capacity of affinity separation, a new approach is used to couple the alkyne-tagged phenylboronic acid to cryogel via an intermediate polymer layer that provides multiple immobilization sites. The morphology and chemical composition of the composite cryogel are characterized systematically. The capability of the composite cryogel for the separation of Gram-positive and Gram-negative bacteria is investigated. The binding capacities of the composite cryogel for Escherichia coli and Staphylococcus epidermidis are 2.15 × 10 9 and 3.36 × 10 9 cfu/g, respectively. The bacterial binding of the composite cryogel can be controlled by adjusting pH. The results suggest that the composite cryogel may be used as affinity medium for rapid separation and isolation of bacteria from complex samples.

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PolyAdenine cryogels for fast and effective RNA purification

Cited by 32 publications

References 39 publications

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

Lead Removal from Industrial Waste

Preparation of Boronic Acid-Functionalized Cryogels Using Modular and Clickable Building Blocks for Bacterial Separation

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