Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Majumdar, P. K.; McFadden, Bruce A.

doi:10.1128/jb.157.3.795-801.1984

Cited by 9 publications

(2 citation statements)

References 47 publications

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“…Nucleic acids were precipitated from the aqueous phase by the addition of 2.5 volumes of ethanol and incubation at -20°C overnight. The precipitate was collected by centrifugation at 27,000 x g for 30 (26). Reaction mixtures were then cooled on ice, diluted by the addition of 300 pl1 of hybridization buffer, and layered onto 17-ml linear gradients of 5 to 20% (wt/vol) sucrose dissolved in 50 mM sodium acetate-150 mM NaCl (pH 5.1).…”

Section: Methodsmentioning

confidence: 99%

“…RNA molecules in RNA preparations from several eubacterial species (12). In contrast to eucaryotic poly(A)+ RNAs, eubacterial poly(A)+ RNAs have much shorter poly(A) tracts, are unstable, exhibit a wide range of sizes, and comprise a much smaller percentage of the total cellular RNA (6,12,19,30,37,41). In several cases, eubacterial poly(A)+ RNAs have been shown by in vitro translation to be functional mRNAs (30,31,45).…”

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Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii

Brown¹,

Reeve²

1985

J Bacteriol

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RNA molecules have been isolated from Methanococcus vannielii by oligodeoxythymidylate-cellulose affinity chromatography at 4C. Approximately 16% of the label in RNA isolated from cultures allowed to incorporate [3H]uridine for 3 min at 37C was poly(A)+ RNA. In contrast, less than 1% of the radioactivity in RNA labeled over a period of several generations was contained in poly(A)+ RNA molecules. Electrophoretic separation of poly(A)+ RNA molecules showed a heterogeneous population with mobilities indicative of sizes ranging from 900 to 3,000 bases in length. The population of poly(A)+ RNA molecules was found to have a half-life in vivo of approximately 12 min. Polyadenylate [poly(A)] tracts were isolated by digestion with RNase A and RNase T, after 3' end labeling of the poly(A)+ RNA with RNA ligase.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii

Brown¹,

Reeve²

1985

J Bacteriol

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Bacterial/archaeal/organellar polyadenylation

Mohanty

Kushner

2010

WIREs RNA

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Although the first poly(A) polymerase (PAP) was discovered in Escherichia coli in 1962, the study of polyadenylation in bacteria was largely ignored for the next 30 years. However, with the identification of the structural gene for E. coli PAP I in 1992, it became possible to analyze polyadenylation using both biochemical and genetic approaches. Subsequently, it has been shown that polyadenylation plays a multifunctional role in prokaryotic RNA metabolism. While the bulk of our current understanding of prokaryotic polyadenylation comes from studies on E. coli, recent experiments with Cyanobacteria, organelles and Archaea, although limited, have widened our view on the diversity, complexity, and universality of the polyadenylation process.For example, the identification of polynucleotide phosphorylase (PNPase), a reversible phosphorolytic enzyme that is highly conserved in bacteria, as an additional PAP in E. coli caught everyone by surprise. In fact, PNPase has now been shown to be the source of post-transcriptional RNA modifications in a wide range of cells of prokaryotic origin including those that lack a eubacterial PAP homologue. Accordingly, the past few years have witnessed increased interest in the mechanism and role of post-transcriptional modifications in all species of prokaryotic origin. However, the fact that many of the poly(A) tails are very short and unstable as well as the presence of polynucleotide tails has posed significant technical challenges to the scientific community trying to unravel the mystery of polyadenylation in prokaryotes. This review discusses the current state of knowledge regarding polyadenylation and its functions in bacteria, organelles and Archaea. Keywords poly(A) polymerase; polynucleotide phosphorylase; Hfq; RNA degradation Polyadenylation is a post-transcriptional event that involves the addition of untemplated adenosine residues to the 3' ends of RNA substrates. The first bacterial poly(A) polymerase (PAP) was identified almost 50 years ago in Escherichia coli [1,2]. A PAP was also identified in eukaryotic cells at about the same time [3,4]. However, polyadenylation in bacteria was virtually ignored for next 30 years, in part because eukaryotic poly(A) tails were relatively long, nearly uniform in length, and were found on almost all mRNAs. Furthermore, even though poly(A) tails were detected in E. coli and Bacillus subtilis [5][6][7][8][9], the overall low abundance of polyadenylated transcripts and the apparent lack of evidence for a physiological role led to the belief that polyadenylation was only important in higher organisms (See definition of polyadenylation in the glossary of Lewin, Genes I through Genes VIII).* To whom correspondence should be addressed. NIH Public Access Author ManuscriptWiley Interdiscip Rev RNA. Author manuscript; available in PMC 2011 September 27. Published in final edited form as:Wiley Interdiscip Rev RNA. 2010 September 27; 2(2): 256-276. doi:10.1002/wrna.51. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscri...

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Transcriptional Regulation of Rubisco Gene Expression by CO2 in Rhodospirillum rubrum

1990

Current Research in Photosynthesis

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Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Cited by 9 publications

References 47 publications

Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii

Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii

Bacterial/archaeal/organellar polyadenylation

Transcriptional Regulation of Rubisco Gene Expression by CO2 in Rhodospirillum rubrum

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