Polyadenylation, methylation, and capping of the RNA synthesized in vitro by African swine fever virus

Salas, María; Kuznar, Juan; Viñuela, Eladio

doi:10.1016/0042-6822(81)90176-8

Cited by 89 publications

(42 citation statements)

References 20 publications

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“…Virion assembly takes place in discrete cytoplasmic areas called viral factories, where the virus becomes engulfed by a two-membraned collapsed cisterna derived from the endoplasmic reticulum (6). Mature particles contain the enzymes required for early RNA synthesis and processing (19,30,31), most of which are known to be encoded by the virus genome (34). ASFV gene expression appears to follow a cascade mechanism similar to that described for poxviruses (22).…”

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confidence: 99%

Structure of African Swine Fever Virus Late Promoters: Requirement of a TATA Sequence at the Initiation Region

García‐Escudero

Viñuela

2000

J Virol

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A number of mutations, including deletions, linker scan substitutions, and point mutations, were performed in the promoter of the late African swine fever virus (ASFV) gene coding for the capsid protein p72. The consequences of the mutations in terms of promoter activity were analyzed by luciferase assays using plasmids transfected into infected cells. The results showed that the promoter function is contained between nucleotides ؊36 and ؉5 relative to the transcription initiation site. Moreover, two major essential regions for promoter activity, centered at positions ؊13 and ؉3, were located along the 41-bp sequence, the latter mapping in the transcription start site. Sequence alignment with other ASFV late promoters showed homology in the region of transcriptional initiation, where the presence of the sequence TATA was observed in most of the promoters. Substitution of these four residues in three other late viral promoters strongly reduced their respective activities. These results show that cis-acting control elements of ASFV p72 gene transcription are restricted to a short sequence of about 40 bp and suggest that transcription of late genes is initiated around a TATA sequence that would function as an initiator element.

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confidence: 99%

Structure of African Swine Fever Virus Late Promoters: Requirement of a TATA Sequence at the Initiation Region

García‐Escudero

Viñuela

2000

J Virol

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“…Meanwhile, transcription of early genes and posttranscriptional modification of the mRNA occur inside the virion; this is ensured by packaged proteins such as the viral RNA polymerase and, hence, independent of cellular enzymes (37)(38)(39)(40)(41). Late gene expression, however, depends on viral DNA replication and viral early proteins.…”

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confidence: 99%

DNA Virus Replication Compartments

Schmid

Speiseder

Dobner

et al. 2014

J Virol

158

142

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bViruses employ a variety of strategies to usurp and control cellular activities through the orchestrated recruitment of macromolecules to specific cytoplasmic or nuclear compartments. Formation of such specialized virus-induced cellular microenvironments, which have been termed viroplasms, virus factories, or virus replication centers, complexes, or compartments, depends on molecular interactions between viral and cellular factors that participate in viral genome expression and replication and are in some cases associated with sites of virion assembly. These virus-induced compartments function not only to recruit and concentrate factors required for essential steps of the viral replication cycle but also to control the cellular mechanisms of antiviral defense. In this review, we summarize characteristic features of viral replication compartments from different virus families and discuss similarities in the viral and cellular activities that are associated with their assembly and the functions they facilitate for viral replication.

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“…Its genome is formed by a double-stranded DNA molecule of about 170 kbp with hairpin loops and terminal inverted repeats (Gonzfilez et al, 1986;Sogo et al, 1984), similar to those of poxvirus DNA (Baroudy et al, 1982;Wittek & Moss, 1980). Like the poxviruses (Moss, 1990), ASF virus packages a complete enzymatic system for the synthesis of early RNA (Kuznar et al, 1980;Salas et al, 1981Salas et al, , 1986.…”

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confidence: 99%

African swine fever virus thymidylate kinase gene: sequence and transcriptional mapping

Yáñez

Rodríguez²,

Rodrı́guez³

et al. 1993

Journal of General Virology

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A putative thymidylate kinase gene of African swine fever virus has been identified at the left end of the Sall I' fragment of the virus genome. The gene, designated A240L, has the potential to encode a protein of 240 amino acids with an M r of 27 754 and is transcribed early after infection. Primer extension analysis indicates that transcription is initiated a short distance from the first ATG codon of open reading frame A240L. The deduced amino acid sequence of this open reading frame shows significant similarity with the human, yeast and vaccinia virus thymidylate kinases, the degree of identity being 23"7, 25 and 23"5 %, respectively. The putative African swine fever virus thymidylate kinase sequence is essentially collinear with the other thymidylate kinase sequences, but contains a carboxy-terminal extension of 37 amino acids rich in glutamic and aspartic acids. The A240L protein conserves the ATP-binding and nucleotide/nucleoside-binding domains characteristic of thymidylate kinases.

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Polyadenylation, methylation, and capping of the RNA synthesized in vitro by African swine fever virus

Cited by 89 publications

References 20 publications

Structure of African Swine Fever Virus Late Promoters: Requirement of a TATA Sequence at the Initiation Region

Structure of African Swine Fever Virus Late Promoters: Requirement of a TATA Sequence at the Initiation Region

DNA Virus Replication Compartments

African swine fever virus thymidylate kinase gene: sequence and transcriptional mapping

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