Polyamidoamine-grafted silica nanoparticles as pseudostationary phases for capillary electrochromatographic separation of proteins

Gao, Jingjie; Latep, Nurgul; Ge, Ying; Jing, Tian; Wu, Jingqing; Qin, Weidong

doi:10.1002/jssc.201200837

Cited by 18 publications

(13 citation statements)

References 40 publications

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“…The Fe 3 O 4 –SiO 2 nanocomposites were allowed to react with 3‐aminopropyltriethoxysilane (APTS, Aladdin, Shanghai, China) to introduce active amine groups to the surfaces. Afterward, various generations of polyamidoamines were grafted to the nanoparticles via alternate Michael addition and amidation . Please refer to Section 1 of the Supporting Information for details.…”

Section: Methodsmentioning

confidence: 99%

DNA fragments assembled on polyamidoamine‐grafted core‐shell magnetic silica nanoparticles for removal of mercury(II) and methylmercury(I)

Liang

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND Hg(II) and MeHg(I) are toxic pollutants causing wide concerns. Polyamidoamine‐grafted magnetic silica nanoparticles (Fe3O4‐SiO2‐PAMAMs) were synthesized as beads, on which herring sperm DNA fragments were immobilized as reacting moieties for removal of Hg(II) and MeHg(I) from water matrices. The factors influencing the removal, such as DNA fragments, generation of the polyamidoamine spacers and pH, were studied. Further, the adsorption mechanisms were explored by means of adsorption kinetics and isotherms. RESULTS Assembling DNA fragments on the polyamidoamine‐grafted beads promoted sharply the removal of both Hg(II) and MeHg(I). Adsorptions of Hg(II) and MeHg(I) followed pseudo‐second‐order kinetics; but their adsorption isotherms obeyed Langmuir and Freundlich models, respectively. Under the optimum conditions, the adsorption equilibrium time was approximately 5 min for Hg(II) and 5 s for MeHg(I) with maximum percentage removals of ∼95% and ∼90%, respectively. The adsorbent could be regenerated easily without significant decrease in removal efficiencies. CONCLUSION The Fe3O4‐SiO2‐PAMAM beads could bind DNA steadily, and the DNA fragments interacted strongly with mercury species. The strategy enabled the water‐soluble DNAs to work as efficient removing reagents for rapid and cost‐effective removal of Hg(II) and MeHg(I). © 2016 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

DNA fragments assembled on polyamidoamine‐grafted core‐shell magnetic silica nanoparticles for removal of mercury(II) and methylmercury(I)

Liang

et al. 2016

J of Chemical Tech & Biotech

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“…In the case of modified‐SiNPs, they have been recently used including SiNPs bonded with only polyamidoamines for the separation of proteins and subsequent functionalization with β‐CD for enantioseparation of three racemic drugs as well as amines‐bonded SiNPs for separation of oligosaccharide derivatives and nucleic acid bases and plant auxins in grapes . In this last case, after peeled off, skived, centrifuged, filtered, and diluted two times with buffer solution, the studied analytes were added obtaining recovery values higher than 88.1% and RSDs for peak areas and separation times lower than 3.2 and 1.9%, respectively.…”

Section: Nanomaterials Applications In Capillary Electrochromatographymentioning

confidence: 99%

Recent applications of nanomaterials in capillary electrophoresis

et al. 2017

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Nanomaterials have found an important place in Analytical Chemistry and, in particular, in Separation Science. Among them, metal-organic frameworks, magnetic and non-magnetic nanoparticles, carbon nanotubes and graphene, as well as their combinations, are the most important nanomaterials that have been used up to now. Concerning capillary electromigration techniques, these nanomaterials have also been used as both pseudostationary phases in electrokinetic chromatography (EKC) and as stationary phases in microchip capillary electrophoresis (CE) and capillary electrochromatography (CEC), as a result of their interesting and particular properties. This review article pretends to provide a general and critical revision of the most recent applications of nanomaterials in this field (period 2010-2017).

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“…A wide range of materials have been investigated for PSP-CEC, including polymer NP, carbon nanotubes, gold NP, and silica NP (SNP) [67]. To improve the stability and functionality of SNP, Gao et al synthesized polyamidoamine-grafted SNP (PAMAM-SNP) and utilized them for a separation of basic and acidic proteins [68] (Fig. 6).…”

Section: Techniquesmentioning

confidence: 99%

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

2014

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The development of therapeutic proteins and peptides is an expensive and time-intensive process. Biologics, which have become a multi-billion dollar industry, are chemically complex products that require constant observation during each stage of development and production. Post-translational modifications along with chemical and physical degradation from oxidation, deamidation, and aggregation, lead to high levels of heterogeneity that affect drug quality and efficacy. The various separation modes of capillary electrophoresis (CE) are commonly utilized to perform quality control and assess protein heterogeneity. This review attempts to highlight the most recent developments and applications of CE separation techniques for the characterization of protein and peptide therapeutics by focusing on papers accepted for publication in the in the two-year period between January 2012 and December 2013. The separation principles and technological advances of CE, capillary gel electrophoresis, capillary isoelectric focusing, capillary electrochromatography and CE-mass spectrometry are discussed, along with exciting new applications of these techniques to relevant pharmaceutical issues. Also included is a small selection of papers on microchip electrophoresis to show the direction this field is moving with regards to the development of inexpensive and portable analysis systems for on-site, high-throughput analysis.

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Polyamidoamine-grafted silica nanoparticles as pseudostationary phases for capillary electrochromatographic separation of proteins

Cited by 18 publications

References 40 publications

DNA fragments assembled on polyamidoamine‐grafted core‐shell magnetic silica nanoparticles for removal of mercury(II) and methylmercury(I)

DNA fragments assembled on polyamidoamine‐grafted core‐shell magnetic silica nanoparticles for removal of mercury(II) and methylmercury(I)

Recent applications of nanomaterials in capillary electrophoresis

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

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