Polyamine levels in red blood cells from patient groups of different sex and age

Elworthy, Paul; Hitchcock, Edward

doi:10.1016/0304-4165(89)90166-9

Cited by 15 publications

(16 citation statements)

References 24 publications

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“…The trend of a slight decrease in the blood spermine concentration with aging was observed among 42 volunteers; however, the concentrations of either spermine or spermidine did not have an inverse correlation with age (53). Blood cells can take up polyamine from the surrounding environment, and the largest source in the body is the intestinal lumen (19 -22).…”

Section: Discussionmentioning

confidence: 99%

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Soda¹,

Kano²,

Nakamura³

et al. 2005

The Journal of Immunology

113

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Natural polyamines, spermine, spermidine, and putrescine, play a pivotal role in the regulation of gene expression; therefore, the age-dependent decreases and the disease-dependent increases in polyamine synthesis suggest a possible contribution of polyamines to the age-related and disease-associated changes in cellular function. In this study, we examined the effects of polyamines on the cellular function and the expression of adhesion molecules on human PBMCs from healthy volunteers. Flow cytometry revealed that PBMCs cultured with spermine decreased mean fluorescent intensities (MFIs) of CD11a and CD18 in the lymphocyte light-scattered region, but not in the monocyte region. This suppression was observed in a dose- and time-dependent manner and found nonspecifically on all cell subsets we tested (CD3+, CD4+, CD8+, CD19+, CD45RA+, CD45RO+, CD4+CD45RA+, CD4+CD45RO+, CD8+CD45RA+, CD8+CD45RO+). The decreases of CD11a and CD18 MFIs were accompanied by the decrease in adherent capacity of PBMCs to HUVECs. Spermine did not hinder cell activities or cell viability. Among 42 healthy volunteers (mean, 49.5 years old; from 26 to 69), blood spermine levels inversely correlated with the CD11a MFIs of cells in the lymphocyte region (r = −0.48; p = 0.001), but not with those in the monocyte region. The effects of spermidine seemed weaker than those of spermine, and blood spermidine levels had no correlation with CD11a MFIs of the lymphocyte region. Putrescine had no effect on the expressions of membrane molecules. Polyamines, especially spermine, decrease LFA-1 (CD11a/CD18) expression on human lymphocyte and adhesion capacity of PBMCs to HUVECs.

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Section: Discussionmentioning

confidence: 99%

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Soda¹,

Kano²,

Nakamura³

et al. 2005

The Journal of Immunology

113

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“…The ability to synthesize polyamines decreases with aging. However, when polyamine concentrations in blood cells are measured in healthy human adults, the aging-associated decline in polyamine concentrations is not remarkable, and large inter-individual differences are found [ 19 , 84 ]. Polyamines exist in almost all living organisms, and thus, foods that are comprised of various types of organisms and their related substances contain polyamines, though at a wide variety of concentrations [ 85 , 86 , 87 , 88 ].…”

Section: Source Of Body Polyaminesmentioning

confidence: 99%

Polyamine Metabolism and Gene Methylation in Conjunction with One-Carbon Metabolism

Soda

2018

IJMS

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Recent investigations have revealed that changes in DNA methylation status play an important role in aging-associated pathologies and lifespan. The methylation of DNA is regulated by DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) in the presence of S-adenosylmethionine (SAM), which serves as a methyl group donor. Increased availability of SAM enhances DNMT activity, while its metabolites, S-adenosyl-l-homocysteine (SAH) and decarboxylated S-adenosylmethionine (dcSAM), act to inhibit DNMT activity. SAH, which is converted from SAM by adding a methyl group to cytosine residues in DNA, is an intermediate precursor of homocysteine. dcSAM, converted from SAM by the enzymatic activity of adenosylmethionine decarboxylase, provides an aminopropyl group to synthesize the polyamines spermine and spermidine. Increased homocysteine levels are a significant risk factor for the development of a wide range of conditions, including cardiovascular diseases. However, successful homocysteine-lowering treatment by vitamins (B6, B12, and folate) failed to improve these conditions. Long-term increased polyamine intake elevated blood spermine levels and inhibited aging-associated pathologies in mice and humans. Spermine reversed changes (increased dcSAM, decreased DNMT activity, aberrant DNA methylation, and proinflammatory status) induced by the inhibition of ornithine decarboxylase. The relation between polyamine metabolism, one-carbon metabolism, DNA methylation, and the biological mechanism of spermine-induced lifespan extension is discussed.

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“…In animal tissue, an aging-associated decline in ODC activity is observed, with a concomitant gradual decrease in polyamine concentration (Das & Kanungo, 1982;Laitinen et al, 1982). However, when polyamine concentrations in whole blood (mainly in erythrocytes and leukocytes) are measured in adult humans, the aging-associated decline in polyamine concentrations is not remarkable, and large inter-individual differences are found (Elworthy & Hitchcock, 1989;Soda et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Biological Effects of Polyamines on the Prevention of Aging-associated Diseases and on Lifespan Extension

Soda

2015

FSTR

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Polyamine levels in red blood cells from patient groups of different sex and age

Cited by 15 publications

References 24 publications

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Polyamine Metabolism and Gene Methylation in Conjunction with One-Carbon Metabolism

Biological Effects of Polyamines on the Prevention of Aging-associated Diseases and on Lifespan Extension

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