Polyamine Metabolism in P388 Leukemia Cells and in Ascites Tumor-Bearing Mice

Kremmer, T.; Selmeci, L.; Bardócz, Susan; Holczinger, L.; Bálint, Susan

doi:10.1159/000163271

Cited by 4 publications

(6 citation statements)

References 11 publications

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“…We have shown earlier that P388/S leuke mia cells grown intrapcritoneally in BDF| mice have an active polyamine metabolism at the early phase or cell proliferation (1-2 days), but a continuous decrease in all poly amine fractions can be observed throughout tumor growth [19,23]. Our present results indicate (table I) that ODC activities of P388/S leukemia cells originating from DFMO-treated mice were very low and just detectable during the whole period of experi- merits.…”

Section: Resultsmentioning

confidence: 91%

“…DFMO hydrochloride monohydrate (RMI 71.782, DFMO kindly donated by Centre de Recherche Merrell International, Stras bourg, France) was dissolved every 2 days in distilled water at a concentration of 2% for administration via drinking water. P388/S leukemia cells were main tained in BDF| mice by serial transplantation in our Institute as previously described [19]-DFMO was given to BDF| mice, 60-70 in each group, in water as sole drinking fluid. 2 weeks before the intraperitoneal transplantation of P388/S leuke mia cells and continued throughout tumor growth.…”

Section: Methodsmentioning

confidence: 99%

“…Aliquots of cells (usually 5, 30 and 50 millions for ODC, polyamine and nucleotide phosphate determinations, respective ly) from pooled cell suspensions of 5-10 mice were collected by centrifugation (5.000 rpm, 0 ®C, 10 min). ODC activity and polyamine content of tumor cells were measured as described earlier [19][20][21]. For deter mination of nucleotide phosphates, tumor cells were washed in phosphate-buffered saline (PBS.…”

Section: Methodsmentioning

confidence: 99%

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In vivo Effects of DL-α-Difluoromethylornithine on the Polyamine and Nucleotide Phosphate Metabolism in P388/S Leukemia Cells

Kremmer¹,

Boldizsár²,

Holczinger³

1986

Pathobiology

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In vivo effects of DL-α-difluoromethylornithine (DFMO) on the metabolism of polyamines and nucleotide phosphates were monitored in P388/S leukemia cells grown intra-peritoneally in BDF₁ inbred male mice. Inhibiting the ornithine decarboxylase (ODC) activity DFMO depleted putrescine and spermidine to 30–50 and 50–60%, respectively, and increased spermine to 25–60% compared with the controls, when given as 2% solution in drinking water of the tumor-bearing animals. DFMO treatment caused a parallel 56% elevation of total nucleotide content in tumor cells with distinct and significant increase of some nucleotide phosphates. The most pronounced alterations were shown in the intracellular UTP (202%), CTP (103%), ADP (92%) and ATP (71%) concentrations. Changes in polyamine and nucleotide phosphate metabolisms were dependent on tumor progression. A possible explanation of the metabolic events induced by DFMO is discussed.

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Section: Resultsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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In vivo Effects of DL-α-Difluoromethylornithine on the Polyamine and Nucleotide Phosphate Metabolism in P388/S Leukemia Cells

Kremmer¹,

Boldizsár²,

Holczinger³

1986

Pathobiology

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“…For vacuumpackaged meat stored at low temperatures, Enterobacteriaceae, especially Hafnia alvei and Serratia liquefaciens, have been identified as playing a major role in the accumulation of diamines (Dainty et al, 1986). The situation is complicated by the fact that socalled polyamines constitute essential compounds of living cells (Kremmer et al, 1984), while other amines cause adverse health effects (Bardocz, 1995) such as histamine (reviewed by Lehane and Olley, 2000) and tyramine (Beutling, 1996). Other amines potentiate such effects or are associated with tumorous growth (spermine, spermidine and putrescine; Kaminski et al, 1987;Seiler, 2003).…”

Section: Introductionmentioning

confidence: 99%

Biogenic Amines and Polyamines and Total Aerobic Count During Storage of Vacuum-Packaged Porcine Kidney, Liver and Spleen

Valero

Bauer

Smulders

et al. 2005

Food sci. technol. int.

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Biogenic amines and polyamines (cadaverine, histamine, 2-phenylethylamine, putrescine, spermidine, spermine, tryptamine and tyramine) were analysed in vacuum-packaged porcine livers, kidneys and spleens stored at 3°C and 7°C (for up to 6 days) or 0°C (for up to 21 days). Total aerobic count, pH and sensory assessment were done in parallel. While histamine, 2-phenylethylamine and tryptamine concentrations were nearly constant, spermidine and spermine showed a moderately declining trend, irrespective of the storage temperature. Cadaverine, putrescine and tyramine concentrations increased with storage time and temperature. Maximum concentrations at day 21 at 0°C were: 122 mg/kg for cadaverine, 207.35 mg/kg for putrescine and 63.19 mg/kg for tyramine. The correlation of concentrations of the latter three amines and the total aerobic count was ranging from r ϭ 0.54 to 0.89. A significant rise in amine concentrations was observed only when the total aerobic count exceeded 6 log 10 cfu/g. Concentrations of cadaverine, putrescine and tyramine may be useful to confirm spoilage of vacuum-packaged inner organs. The fraction of the potential food-borne pathogen Aeromonas in high-pH organs (spleen, kidney) during storage was significantly higher than in liver, with low pH. It was observed that the spermine : spermidine ratio of spleen (3 : 2; weight base) was significantly different from that of liver and kidney (4 : 1).

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“…(1) P388 cells in culture represent a poly amine pattern similar to those growing in vivo [8], characterized by the predominance of Spd, while Pu and Spn contributed to the total polyamines in a smaller extent only.…”

Section: Resultsmentioning

confidence: 97%

Changes in the Polyamine and Nucleotide Metabolism of P388 Leukemia Cells Treated with DL-α-Difluoromethylornithine in Culture

et al. 1988

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Influence of DL-α-difiuoromethylornithine (DFMO) treatment on the growth kinetics, labelling index, extra- and intracellular polyamine and nucleotide concentrations was monitored in cultured P388 leukemia cells. A substantial decrease of cell proliferation was observed when the cells were continuously treated with 1–5 mM DFMO. Depletion of cellular polyamines, mostly of putrescine and spermidine, was seen with a concomitant but delayed increase of spermidine and spermine levels in the culture medium. Changes of DNA content and of labelling index of untreated and treated cells seem to indicate that DFMO arrested cells in G₁/S transition. The results presented here provide additional in vitro evidence on the characteristic changes in the metabolic imbalance of ornithine in tumor cells induced by DFMO via inhibition of ornithine decarboxylase and ornithine carbamoyl transferase activities.

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Polyamine Metabolism in P388 Leukemia Cells and in Ascites Tumor-Bearing Mice

Cited by 4 publications

References 11 publications

In vivo Effects of <i>DL</i>-α-Difluoromethylornithine on the Polyamine and Nucleotide Phosphate Metabolism in P388/S Leukemia Cells

In vivo Effects of <i>DL</i>-α-Difluoromethylornithine on the Polyamine and Nucleotide Phosphate Metabolism in P388/S Leukemia Cells

Biogenic Amines and Polyamines and Total Aerobic Count During Storage of Vacuum-Packaged Porcine Kidney, Liver and Spleen

Changes in the Polyamine and Nucleotide Metabolism of P388 Leukemia Cells Treated with <i>DL</i>-α-Difluoromethylornithine in Culture

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