Polyamine patterns in the cerebrospinal fluid of patients with Parkinson's disease and multiple system atrophy

Paik, Man‐Jeong; Ahn, Young Hwan; Lee, Phil Hyu; Kang, Hyun-Seung; Park, Chan Bae; Choi, Sangdun; Lee, Gwang

doi:10.1016/j.cca.2010.05.034

Cited by 68 publications

(67 citation statements)

References 19 publications

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“…Paik et al (2010) measured several polyamines in the CSF of patients with PD, MSA and controls. These substances are important for cell growth, and act as important modulators of a variety of ion channels, including glutamate NMDA and AMPA receptors.…”

Section: Other Compoundsmentioning

confidence: 99%

Cerebrospinal fluid biochemical studies in patients with Parkinson's disease: toward a potential search for biomarkers for this disease

Jiménez‐Jiménez¹,

Alonso‐Navarro

García‐Martín

et al. 2014

Front. Cell. Neurosci.

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The blood-brain barrier supplies brain tissues with nutrients and filters certain compounds from the brain back to the bloodstream. In several neurodegenerative diseases, including Parkinson's disease (PD), there are disruptions of the blood-brain barrier. Cerebrospinal fluid (CSF) has been widely investigated in PD and in other parkinsonian syndromes with the aim of establishing useful biomarkers for an accurate differential diagnosis among these syndromes. This review article summarizes the studies reported on CSF levels of many potential biomarkers of PD. The most consistent findings are: (a) the possible role of CSF urate on the progression of the disease; (b) the possible relations of CSF total tau and phosphotau protein with the progression of PD and with the preservation of cognitive function in PD patients; (c) the possible value of CSF beta-amyloid 1-42 as a useful marker of further cognitive decline in PD patients, and (d) the potential usefulness of CSF neurofilament (NFL) protein levels in the differential diagnosis between PD and other parkinsonian syndromes. Future multicentric, longitudinal, prospective studies with long-term follow-up and neuropathological confirmation would be useful in establishing appropriate biomarkers for PD.

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Section: Other Compoundsmentioning

confidence: 99%

Cerebrospinal fluid biochemical studies in patients with Parkinson's disease: toward a potential search for biomarkers for this disease

Jiménez‐Jiménez¹,

Alonso‐Navarro

García‐Martín

et al. 2014

Front. Cell. Neurosci.

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“…AC3 has been described in kidney and in spermatozoa [42], and CNG2 in the cochlea [38], in vascular tissue [39] and in the brain [43,44]. As none of the ORs present in the CP have identified ligands, we investigated the putative functionality of the transduction pathway by evaluating electrophysiological responses in mice CP explants to a set of polyamines occurring naturally in the CSF [25,26], which are also known to elicit underwater olfactory responses in the fish olfactory epithelium [23,24,45].…”

Section: Discussionmentioning

confidence: 99%

“…The discovery of a novel CSF surveillance mechanism in CP, where ORs and TAARs may function as sensors and may play a physiological role in regulating aspects of CSF composition, may be very relevant. Polyamines naturally occur in the CSF and changes in their concentrations have been associated with neurodegenerative diseases and stroke [25,26]. Physiological polyamines (cadaverine, putrescine, spermine and spermidine) are closely related to neuronal cell activity in the brain, including protection of neuronal cells from oxidative damage, interaction with neurotransmitter receptors, adjustment of substances in degenerating cells and calcium flux regulation [51][52][53][54].…”

Section: Discussionmentioning

confidence: 99%

“…The functionality of the olfactory transduction pathway(s) was evaluated in brain-CP explants by electrophysiological studies, using a set of polyamines as stimuli. These odorants were chosen because they are detected by the piscine olfactory epithelium in the water [23,24], and are also components of the CSF, whose levels are altered in brain disease [25,26]. Crucially, CP electrophysiological responses indicate that the olfactory-type chemosensory apparatus is functional through two different transduction pathways, the AC3/cAMP and the PLC/ IP 3 pathways, and possibly a third, unexplored, transduction mechanism.…”

Section: Introductionmentioning

confidence: 99%

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‘Smelling’ the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus

et al. 2016

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The olfactory-type signaling machinery has been known to be involved not only in odorant detection but also in other tissues with unsuspected sensory roles. As a barrier, the choroid plexus (CP) is an active participant in the monitoring of the cerebrospinal fluid (CSF), promptly responding to alterations in its composition. We hypothesized that olfactory signaling could be active in CP, contributing to the surveillance of the CSF composition. We determined the mRNA and protein expression of the major components of the olfactory transduction pathway in the rat CP, including odorant receptors, the olfactory G-protein (Gaolf), adenylate cyclase 3 and cyclic nucleotide-gated channel 2. The functionality of the transduction pathway and the intracellular mechanisms involved were analyzed by DC field potential recording electrophysiological analysis, in an ex vivo CP-brain setup, using polyamines as stimuli and blockers of the downstream signaling pathways. Concentration-dependent responses were obtained for the polyamines studied (cadaverine, putrescine, spermine and spermidine), all known to be present in the CSF. Transfection of a CP epithelial cell line with siRNA against Gaolf effectively knocked down protein expression and reduced the CP cells' response to spermine. Thus, the key components of the olfactory chemosensory apparatus are present and are functional in murine CP, and polyamines seem to trigger both the cAMP and the phospholipase C-inositol 1,4,5-trisphosphate pathways. Olfactory-like chemosensory signaling may be an essential component of the CP chemical surveillance apparatus to detect alterations in the CSF composition, and to elicit responses to modulate and maintain brain homeostasis.

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“…AA levels in hMSCs-T were normalized to the corresponding mean in the hMSCs group. Subsequently, each normalized value was plotted as a line radiating from a common central point, and the far ends of the lines were joined together to produce star patterns using MS Excel as described elsewhere [18][19][20].…”

Section: Star Symbol Plottingmentioning

confidence: 99%

Characterization of a growth-elevated cell line of human bone marrow-derived mesenchymal stem cells by SV40 T-antigen

Lee

Shim

Paik

et al. 2015

Biotechnol Bioproc E

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Human bone marrow-derived mesenchymal stem cells (hMSCs) are capable of self-renewal and differentiation into various tissue lineages, attracting attention as tools for use in cell therapy. However, hMSCs have very poor proliferative capacity and a short life span in culture. To overcome this problem, we expressed the T antigen of SV40 in hMSCs because it is known to have the ability to elevate the growth rate of various primary animal cells. We obtained several hMSCs lines (hMSCs-T) known for stable expression of T antigen. Cells expressing T antigen proliferated on the monolayer of hMSCs, forming high density foci. hMSCs-T showed changed morphology and improved growth rate and life span, and demonstrated preservation of the potential for differentiation into osteoblasts. In addition, hMSCs-T did not proliferate in soft agar culture, indicating that the cells did not transform into tumor cells. In order to evaluate metabolic change of amino acids in hMSCs-T compared to primary hMSCs, we investigated altered amino acids (AA) with gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode (GC-SIM-MS). A total of 14 AAs were positively measured. Results from the Student's t-test on the hMSCs group mean of the hMSCs-T group showed significantly elevated levels of glycine, proline, pipecolic acid, aspartic acid, lysine and tryptophan, whereas valine, leucine and isoleucine as branched-chain amino acids (BCAAs), and phenylalanine showed a significant decrease. Altered AAs metabolic pattern in the hMSCs-T may explain the disturbance of AA metabolism related to the expression of SV40 T antigen in hMSCs.

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Polyamine patterns in the cerebrospinal fluid of patients with Parkinson's disease and multiple system atrophy

Cited by 68 publications

References 19 publications

Cerebrospinal fluid biochemical studies in patients with Parkinson's disease: toward a potential search for biomarkers for this disease

Cerebrospinal fluid biochemical studies in patients with Parkinson's disease: toward a potential search for biomarkers for this disease

‘Smelling’ the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus

Characterization of a growth-elevated cell line of human bone marrow-derived mesenchymal stem cells by SV40 T-antigen

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