Polyamine synthesis in plants: isolation and characterization of spermidine synthase from soybean (Glycine max) axes

Yun, Sang Oh; Lee, Yong Sun; Lee, Sung Ho; Cho, Young Dong

doi:10.1016/s0304-4165(00)00039-8

Cited by 45 publications

(19 citation statements)

References 36 publications

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“…The size of the amplification fragment was the same as that of the introduced gene (PC), confirming that all lines of transgenic plants we obtained had successfully incorporated the FSPD1 gene. Immunoblot analysis with the antibody raised against C. ficifolia SPDS revealed the expression in transgenic plants of an immunoreactive protein of 35.5 kDa ( Figure 1B), which corresponded, in terms of molecular mass, to soybean SPDS (Yoon et al 2000). The phenotypes of transgenic plants were not greatly different from those of WT plants, but the internode was characteristically shorter in the former.…”

Section: Analysis Of the 35s:fspd1 Plantsmentioning

confidence: 97%

Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase

Kasukabe

Watakabe

et al. 2006

Plant Biotechnology

113

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Section: Analysis Of the 35s:fspd1 Plantsmentioning

confidence: 97%

Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase

Kasukabe

Watakabe

et al. 2006

Plant Biotechnology

113

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“…2A). Immunoblot analysis with an antibody raised against C. ficifolia SPDS revealed the presence of an immunoreactive protein of 35.5 kDa, which corresponded, in terms of molecular mass, to soybean SPDS (Yoon et al 2000) (Fig. 2B).…”

Section: Analysis Of the 35s:fspd1 Plantsmentioning

confidence: 99%

Overexpression of Spermidine Synthase Enhances Tolerance to Multiple Environmental Stresses and Up-Regulates the Expression of Various Stress-Regulated Genes in Transgenic Arabidopsis thaliana

Kasukabe¹,

He²,

Nada³

et al. 2004

Plant and Cell Physiology

547

312

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;Polyamines play pivotal roles in plant defense to environmental stresses. However, stress tolerance of genetically engineered plants for polyamine biosynthesis has been little examined so far. We cloned spermidine synthase cDNA from Cucurbita ficifolia and the gene was introduced to Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. The transgene was stably integrated and actively transcribed in the transgenic plants. As compared with the wild-type plants, the T2 and T3 transgenic plants exhibited a significant increase in spermidine synthase activity and spermidine content in leaves together with enhanced tolerance to various stresses including chilling, freezing, salinity, hyperosmosis, drought, and paraquat toxicity. During exposure to chilling stress (5°C), the transgenics displayed a remarkable increase in arginine decarboxylase activity and conjugated spermidine contents in leaves compared to the wild type. A cDNA microarray analysis revealed that several genes were more abundantly transcribed in the transgenics than in the wild type under chilling stress. These genes included those for stress-responsive transcription factors such as DREB and stress-protective proteins like rd29A. These results strongly suggest an important role for spermidine as a signaling regulator in stress signaling pathways, leading to build-up of stress tolerance mechanisms in plants under stress conditions.

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“…3A; Fontecave et al 2004). In the pre-biotic RNA world, this might have been a key role for a SAM-dependent ribozyme, as both spermine and spermidine are synthesized from SAM (Yoon et al 2000). These positively charged, polyvalent amines are known to interact with RNA, DNA, and phospholipids in vivo (Loenen 2006).…”

Section: S-adenosyl Methioninementioning

confidence: 99%

Riboswitch effectors as protein enzyme cofactors

Cochrane¹,

Strobel²

2008

RNA

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The recently identified glmS ribozyme revealed that RNA enzymes, like protein enzymes, are capable of using small molecules as catalytic cofactors to promote chemical reactions. Flavin mononucleotide (FMN), S-adenosyl methionine (SAM), adenosyl cobalamin (AdoCbl), and thiamine pyrophosphate (TPP) are known ligands for RNA riboswitches in the control of gene expression, but are also catalytically powerful and ubiquitous cofactors in protein enzymes. If RNA, instead of just binding these molecules, could harness the chemical potential of the cofactor, it would significantly expand the enzymatic repertoire of ribozymes. Here we review the chemistry of AdoCbl, SAM, FMN, and TPP in protein enzymology and speculate on how these cofactors might have been used by ribozymes in the prebiotic RNA World or may still find application in modern biology.

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Polyamine synthesis in plants: isolation and characterization of spermidine synthase from soybean (Glycine max) axes

Cited by 45 publications

References 36 publications

Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase

Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase

Overexpression of Spermidine Synthase Enhances Tolerance to Multiple Environmental Stresses and Up-Regulates the Expression of Various Stress-Regulated Genes in Transgenic Arabidopsis thaliana

Riboswitch effectors as protein enzyme cofactors

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