Polyamines and ethylene metabolism during cold acclimation in zoysiagrass (Zoysia Japonica Steud.)

Sun, Xinbo; Li, Xin; Zhu, Junfei; Huang, Ning; Xiu-ju, Bian; Li, Huibin; Wang, Lihong; Han, Liebao

doi:10.1007/s11738-020-03122-z

Cited by 10 publications

(6 citation statements)

References 57 publications

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“…Zoysiagrass is primarily used in golf courses and lawns because of its fine texture, fast spreading and high density. Efforts are ongoing to breed for and select cultivars that have excellent turf quality, late winter dormancy, early spring green‐up and cold hardiness (Jin et al, 2022; Sun et al, 2020). The stay‐green ( SGR ) phenotype is a highly desirable trait in zoysiagrasses; however, the trait is lacking in these genetic resources.…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium‐mediated transformation via establishment of stable tissue culture system inZoysia matrella(L.) Merrill ‘Wakaba’

Gondo

Ushiyama

et al. 2022

Grassland Science

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Zoysia matrella (L.) Merrill is a perennial C4 warm-season turfgrass grown for landscapes, golf courses, sports fields and recreation parks. To create a new breeding method by genome editing, an efficient genetic transformation system is essential. In this study, we report the efficient protocol of Agrobacterium-mediated transformation through the establishment of a stable tissue culture system for Z. matrella 'Wakaba'.The embryogenic callus was induced from shoot apices of nodal segments incubated in Murashige and Skoog (MS) medium containing 2 mg/l 2,4-dichlorophnoxyacetic acid (2,4-D), 0.1 mg/l 6-benzylaminopurine (BAP) and 5 μM CuSO 4 (MS-DBC).Repeated subculture of compact high-quality callus in MS-DBC medium produced a highly regenerative callus with dense pre-embryogenic clusters, and it was used as a transformation target. Agrobacterium strain EHA105 harboring pANIC8B vector containing the β-glucuronidase gene (GUS) and hygromycin B phosphotransferase gene was used. Agrobacterium-infected calli were cocultured for 5 days with 100 μM acetosyringone and then subjected to selection pressure of 50 mg/l hygromycin. This optimized protocol yielded transformation efficiencies of up to 6.6%. Southern blot analysis verified one to three copies of the GUS gene in different independent transgenic plants. All transgenic plants were morphologically normal, and the GUS expressions were stable. Our optimized in vitro and transgenic system will facilitate the new breeding technology of genome editing in zoysiagrass.

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Section: Discussionmentioning

confidence: 99%

Agrobacterium‐mediated transformation via establishment of stable tissue culture system inZoysia matrella(L.) Merrill ‘Wakaba’

Gondo

Ushiyama

et al. 2022

Grassland Science

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“…They have dual effects, such as being protectors and perpetrators of stress damage to the cells. It has been demonstrated that PAs play a role in providing cold tolerance [ 45 , 46 ]. In this work, we found that amine metabolism related genes showed specific DAS events (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The accumulation of ROS was frequently accompanied by an increase in polyamines during cold acclimation [ 46 ]. ROS, such as H 2 O 2 , can be produced by polyamine catabolism processes.…”

Section: Discussionmentioning

confidence: 99%

Full-length transcriptome analysis of maize root tips reveals the molecular mechanism of cold stress during the seedling stage

Chen

et al. 2022

BMC Plant Biol

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Background As maize originated in tropical or subtropical zones, most maize germplasm is extremely sensitive to low temperatures during the seedling stage. Clarifying the molecular mechanism of cold acclimation would facilitate the breeding of cold tolerant maize varieties, which is one of the major sustainability factors for crop production. To meet this goal, we investigated two maize inbred lines with contrasting levels of cold tolerance at the seedling stage (IL85, a cold tolerant line; B73, a cold sensitive line), and performed full-length transcriptome sequencing on the root tips of seedlings before and after 24 h of cold treatment. Results We identified 152,263 transcripts, including 20,993 novel transcripts, and determined per-transcript expression levels. A total of 1,475 transcripts were specifically up-regulated in the cold tolerant line IL85 under cold stress. GO enrichment analysis revealed that 25 transcripts were involved in reactive oxygen species (ROS) metabolic processes and 15 transcripts were related to the response to heat. Eight genes showed specific differential alternative splicing (DAS) in IL85 under cold stress, and were mainly involved in amine metabolism. A total of 1,111 lncRNAs were further identified, 62 of which were up-regulated in IL85 or B73 under cold stress, and their corresponding target genes were enriched in protein phosphorylation. Conclusions These results provide new insights into the molecular mechanism of cold acclimation during the seedling stage in maize, and will facilitate the development of cultivars with improved cold stress tolerance.

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“…Polyamines have been shown to regulate ethylene biosynthesis by inhibiting ACC accumulation. In addition, the common precursors sharing PAs and ethylene have antagonistic effects: PAs inhibit plant cell senescence, while ethylene promotes it [37][38][39].…”

Section: The Level Of Ethylene Emissionmentioning

confidence: 99%

Dynamics of Polyamines, Proline, and Ethylene Metabolism under Increasing Cold in Winter Oilseed Rape

Jankovska-Bortkevič

Jurkonienė

Gavelienė

et al. 2023

IJMS

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Cold stress is among the most important environmental factors reducing the yield of crops. The present study aimed to investigate the impact of increasing cold stress conditions on winter oilseed rape polyamines, proline, and ethylene metabolism in acclimated and non-acclimated winter oilseed rape. This study was carried out under controlled conditions in the laboratory. The winter oilseed rape hybrid ‘Visby’ was used in the experiment. Acclimated and non-acclimated plants were subjected to a two-day-long increasing cold (from −1 °C to −3 °C) treatment. HPTLC, RT-qPCR, spectral analysis, and gas chromatography methods were used to analyse the levels of polyamines, gene expression, proline, and ethylene, respectively. This study showed a decrease in putrescine, spermidine, and spermine content during cold acclimation and a decrease in putrescine and spermidine levels at sub-zero temperatures. There were intensive changes in ADC2 gene expression, proline, and ethylene levels in non-acclimated plants: a substantial increase after exposure to −1 °C temperature and a sharp decrease after exposure to −3 °C temperature. The changes in these parameters were lower or absent in acclimated plants. The phenomena observed in this study add new insights to the knowledge about the plant stress response and suggest questions to be answered in the future.

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Polyamines and ethylene metabolism during cold acclimation in zoysiagrass (Zoysia Japonica Steud.)

Cited by 10 publications

References 57 publications

Agrobacterium‐mediated transformation via establishment of stable tissue culture system inZoysia matrella(L.) Merrill ‘Wakaba’

Agrobacterium‐mediated transformation via establishment of stable tissue culture system inZoysia matrella(L.) Merrill ‘Wakaba’

Full-length transcriptome analysis of maize root tips reveals the molecular mechanism of cold stress during the seedling stage

Dynamics of Polyamines, Proline, and Ethylene Metabolism under Increasing Cold in Winter Oilseed Rape

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