Polyamines as anabolic growth regulators revealed by transcriptome analysis and metabolite profiles of tomato fruits engineered to accumulate spermidine and spermine

Srivastava, Alka; Chung, Sang Ho; Fatima, Tahira; Datsenka, Tatsiana; Handa, Avtar K.; Mattoo, Autar K.

doi:10.5511/plantbiotechnology.24.57

Cited by 36 publications

(37 citation statements)

References 33 publications

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“…S1, S2). Previous data have demonstrated that high PA levels are linked to a differential accumulation of specific gene transcripts that encompass a wide variety of cellular processes and that PA are endogenous inducers and novel signal transmitters (Srivastava et al 2007;Handa and Mattoo 2010). In this sense, it cannot be ruled out that ET-dependent Put formation could act as a second messenger modulating signal transduction and affecting various morphological and physiological processes in the cell walls during maturefruit abscission.…”

Section: Pa Metabolism During Et-induced Mature-fruit Abscissionmentioning

confidence: 90%

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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Exogenous ethylene and some inhibitors of polyamine biosynthesis can induce mature-fruit abscission in olive, which could be associated with decreased nitric oxide production as a signaling molecule. Whether H₂O₂ also plays a signaling role in mature-fruit abscission is unknown. The possible involvement of H₂O₂ and polyamine in ethylene-induced mature-fruit abscission was examined in the abscission zone and adjacent cells of two olive cultivars. Endogenous H₂O₂ showed an increase in the abscission zone during mature-fruit abscission, suggesting that accumulated H₂O₂ may participate in abscission signaling. On the other hand, we followed the expression of two genes involved in the polyamine biosynthesis pathway during mature-fruit abscission and in response to ethylene or inhibitors of ethylene and polyamine. OeSAMDC1 and OeSPDS1 were expressed differentially within and between the abscission zones of the two cultivars. OeSAMDC1 showed slightly lower expression in association with mature-fruit abscission. Furthermore, our data show that exogenous ethylene or inhibitors of polyamine encourage the free putrescine pool and decrease the soluble-conjugated spermidine, spermine, homospermidine, and cadaverine in the olive abscission zone, while ethylene inhibition by CoCl₂ increases these soluble conjugates, but does not affect free putrescine. Although the impact of these treatments on polyamine metabolism depends on the cultivar, the results confirm that the mature-fruit abscission may be accompanied by an inhibition of S-adenosyl methionine decarboxylase activity, and the promotion of putrescine synthesis in olive abscission zone, suggesting that endogenous putrescine may play a complementary role to ethylene in the normal course of mature-fruit abscission.

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Section: Pa Metabolism During Et-induced Mature-fruit Abscissionmentioning

confidence: 90%

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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“…This titer corresponded to approximately nine haploid genome equivalents and would thus be expected to contain any single copy sequence with [99% probability. The complete, unamplified library was screened by plaque hybridization with a 0.45 kb class I shsp17.6 (Sl17.6 CI) cDNA probe derived from tomato fruit Srivastava et al 2007). Screening of the entire unamplified library resulted in the isolation of a single-phage clone that strongly hybridized with the probe under stringent washing conditions (65°C in 29 SSC, 0.1% SDS for 20 min followed by a single wash in 0.29 SSC, 0.1% SDS for 30 min).…”

Section: Plant Materials and Rna Isolationmentioning

confidence: 99%

Features of a unique intronless cluster of class I small heat shock protein genes in tandem with box C/D snoRNA genes on chromosome 6 in tomato (Solanum lycopersicum)

Goyal

Kumar

Shukla

et al. 2011

Planta

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Physical clustering of genes has been shown in plants; however, little is known about gene clusters that have different functions, particularly those expressed in the tomato fruit. A class I 17.6 small heat shock protein (Sl17.6 shsp) gene was cloned and used as a probe to screen a tomato (Solanum lycopersicum) genomic library. An 8.3-kb genomic fragment was isolated and its DNA sequence determined. Analysis of the genomic fragment identified intronless open reading frames of three class I shsp genes (Sl17.6, Sl20.0, and Sl20.1), the Sl17.6 gene flanked by Sl20.1 and Sl20.0, with complete 5' and 3' UTRs. Upstream of the Sl20.0 shsp, and within the shsp gene cluster, resides a box C/D snoRNA cluster made of SlsnoR12.1 and SlU24a. Characteristic C and D, and C' and D', boxes are conserved in SlsnoR12.1 and SlU24a while the upstream flanking region of SlsnoR12.1 carries TATA box 1, homol-E and homol-D box-like cis sequences, TM6 promoter, and an uncharacterized tomato EST. Molecular phylogenetic analysis revealed that this particular arrangement of shsps is conserved in tomato genome but is distinct from other species. The intronless genomic sequence is decorated with cis elements previously shown to be responsive to cues from plant hormones, dehydration, cold, heat, and MYC/MYB and WRKY71 transcription factors. Chromosomal mapping localized the tomato genomic sequence on the short arm of chromosome 6 in the introgression line (IL) 6-3. Quantitative polymerase chain reaction analysis of gene cluster members revealed differential expression during ripening of tomato fruit, and relatively different abundances in other plant parts.

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“…Polyamines (PAs) are polycationic, ubiquitous compounds that have essential functions in all organisms studied thus far involving regulation at both transcriptional and translational levels (Veress et al, 2000;Kasukabe et al, 2004;Yoshida et al, 2004;Alcázar et al, 2005;Igarashi and Kashiwagi, 2006;Srivastava et al, 2007;Mattoo and Handa, 2008;Handa and Mattoo, 2010). Putrescine (Put), spermidine (Spd), and spermine (Spm) are the three most prominent PAs in plants.…”

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Polyamines Attenuate Ethylene-Mediated Defense Responses to Abrogate Resistance to Botrytis cinerea in Tomato

et al. 2011

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Transgenic tomato (Solanum lycopersicum) lines overexpressing yeast spermidine synthase (ySpdSyn), an enzyme involved in polyamine (PA) biosynthesis, were developed. These transgenic lines accumulate higher levels of spermidine (Spd) than the wild-type plants and were examined for responses to the fungal necrotrophs Botrytis cinerea and Alternaria solani, bacterial pathogen Pseudomonas syringae pv tomato DC3000, and larvae of the chewing insect tobacco hornworm (Manduca sexta). The Spd-accumulating transgenic tomato lines were more susceptible to B. cinerea than the wild-type plants; however, responses to A. solani, P. syringae, or M. sexta were similar to the wild-type plants. Exogenous application of ethylene precursors, S-adenosylMet and 1-aminocyclopropane-1-carboxylic acid, or PA biosynthesis inhibitors reversed the response of the transgenic plants to B. cinerea. The increased susceptibility of the ySpdSyn transgenic tomato to B. cinerea was associated with down-regulation of gene transcripts involved in ethylene biosynthesis and signaling. These data suggest that PA-mediated susceptibility to B. cinerea is linked to interference with the functions of ethylene in plant defense.

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Polyamines as anabolic growth regulators revealed by transcriptome analysis and metabolite profiles of tomato fruits engineered to accumulate spermidine and spermine

Cited by 36 publications

References 33 publications

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Features of a unique intronless cluster of class I small heat shock protein genes in tandem with box C/D snoRNA genes on chromosome 6 in tomato (Solanum lycopersicum)

Polyamines Attenuate Ethylene-Mediated Defense Responses to Abrogate Resistance to Botrytis cinerea in Tomato

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