Polyamines in colorectal cancer. Evaluation of polyamine concentrations in the colon tissue, serum, and urine of 50 patients with colorectal cancer

Löser, Christian; Fölsch, Ulrich R.; Paprotny, Christoph; Creutzfeldt, W.

doi:10.1002/1097-0142(19900215)65:4<958::aid-cncr2820650423>3.0.co;2-z

Cited by 87 publications

(65 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study indicated that the amount of polyamines excreted in the urine was higher in patients with cancer compared to that in healthy individuals (3). However, results of subsequent studies showed that the total amount, as well as the amount of individual free and monoacetylated polyamines in the urine, were not suitable as reliable tumor markers, as these yielded a number of false positive and negative results (4,5). N 1 ,N 12 -diacetylspermine (DiAcSpm) is a minor component of human urine that constitutes less than 0.5% of the total polyamine species in normal human urine.…”

Section: Introductionmentioning

confidence: 86%

The clinical usefulness of urinary N1,N12-diacetylspermine (DiAcSpm) levels as a tumor marker in patients with colorectal cancer

et al. 2012

View full text Add to dashboard Cite

Abstract. The aim of this study was to evaluate the usefulness of urinary N 1 ,N 12 -diacetylspermine (DiAcSpm) measured by the colloidal gold aggregation method as a tumor marker for colorectal cancer (CRC). The preoperative urine of 113 CRC patients was collected, and the urinary DiAcSpm was measured by a reagent kit for DiAcSpm determination based on colloidal gold aggregation using automatic biochemical analyzers. The urinary DiAcSpm levels significantly correlated with distant metastasis and Tumor-Node-Metastasis (TNM) stage. The positive rates of urinary DiAcSpm were significantly higher than those of serum carcinoembryonic antigen (CEA) or cancer antigen 19-9 (CA19-9) in stages 0+Ⅰ, II, III and IV. The positive rates of urinary DiAcSpm were also significantly higher than those of serum CEA or CA19-9 in the early and advanced CRC groups according to the Japan Classification of Colorectal Cancer. Therefore, urinary DiAcSpm, measured by a reagent kit for DiAcSpm determination based on colloidal gold aggregation, may be useful as a non-invasive tumor marker in patients with CRC.

show abstract

Section: Introductionmentioning

confidence: 86%

The clinical usefulness of urinary N1,N12-diacetylspermine (DiAcSpm) levels as a tumor marker in patients with colorectal cancer

et al. 2012

View full text Add to dashboard Cite

show abstract

“…This led to a great flurry of activity examining the possibility that polyamines in body fluids may be biological markers of malignancy (for reviews, see Janne et al 1978;Pegg, 1988). Unfortunately, as a marker, polyamines, particularly urinary polyamines, were nonspecific with false positives being observed in patients with gastrointestinal diseases (Loser et al 1990) and during pregnancy (Russell et al 1971).…”

Section: Cancermentioning

confidence: 99%

Polyamines in human health

Wallace

1996

Proc. Nutr. Soc.

View full text Add to dashboard Cite

Les polyamines dans la santC de l'homme R E S U M ELes polyamines sont essentielles pour la croissance de toutes les cellules vivantes. Elles sont synthCtisCes de n o w par une sCrie de reactions de dCcarboxylation et d'aminopropyl transfkrase, ou sont absorbCes de l'alimentation et de la flore intestinale. I1 existe une relation positive entre les taux de la croissance des cellules et le contenu intracellulaire des polyamines. En gCnCral, le contenu en polyamines est ClevC dans les cellules qui grandissent rapidement, et faible dans les cellules en repos. On trouve couramment des concentrations de polyamines ClevCes dans les cellules et tissus de cancer, mais la fonction prCcise des polyamines dans les cellules canckreuses n'est pas Claire. Le contenu en polyamines dans le systbme urinaire est Cgalement augment6 dans les maladies malignes, et ceci peut donner des informations sur la rCponse des patients individuels au traitement, bien qu'il ne puisse pas servir B 1'Ctablissement du diagnostic. La voie de la biosynthbse des polyamines est une cible pour des agents anticandreux spkcifiquement conps, le mieux utilisC d'entre eux itant 1'wdifluoromCthylornithine (DFMO) qui inhibe la dCcarboxylase de l'ornithine (EC 4.1.1.17), la premikre enzyme limitante dans cette voie. La DFMO est un agent antiproliferant in v i m mais ses effets sont plus cytostatiques que cytotoxiques. Chez l'homme, l'utilisation de la DFMO en monothCrapie comme agent anticanckreux a CtC decevante, mais, parce qu'il n'est pas toxique, le DFMO a CtC combine avec un certain nombre d'autres medicaments parmi lesquels le nitrosourke, le mCthylglyoxa1 bis(guany1hydrazone) (MGBG) et la cyclosporine A, produisant une ClCvation des effets antitumoraux. On a synthCtisC de nouveaux inhibiteurs compCtitifs de la voie vers la dkcarboxylase d'adCnosylmCthionine-S et basis et sur la structure du MGBG. Ceux-ci amCliorent significativement I'activitC antitumorale sur la DFMO ou son parent le MGBG. De plus, ces nouveaux inhibiteurs de la dkcarboxylase d'adCnosylmCthionine-S (EC 4.1.1 S O ) ont montrC une toxicit6 moins importante que le MGBG. Une autre stratCgie pour l'klimination des polyamines dans les cellules est l'utilisation des analogues des polyamines, trbs notoirement les dCrivCs bis(Cthy1s). Ces analogues agissent de plusieurs manikres: inhibition de la biosynthkse en retour, induction d'acCtyltransfCrase N' spermidinehpermine, et couplage aux sites intracellulaires. Ces analogues sont captCs par le transporteur des polyamines mais ne remplacent pas les polyamines en terme de fonction. Une strategic alternative vise 2t Climiner totalement les polyamines en combinant les inhibiteurs de la biosynthbse des polyamines et les reconvertir avec des antibiotiques et un rCgime sans polyamines. La encore, le succbs a CtC obtenu chez des modbles animaux. La DFMO peut Cgalement &re un agent chimioprkventif chez des patients qui ont un risque ClevC de dCvelopper un cancer. On peut soutenir que l'utilisation la plus bCnCfique des inhibiteurs des polyhttps://www.cam...

show abstract

“…High polyamine levels have been detected in most types of cancers (7)(8)(9). In colorectal cancer, the polyamine contents are increased 3-to 4-fold over those found in the equivalent normal tissue (10,11). Depletion of the intracellular polyamine pools invariably inhibits cell growth.…”

Section: Introductionmentioning

confidence: 99%

Adenovirus-mediated expression of spermidine/spermine N1-acetyltransferase gene induces S-phase arrest in human colorectal cancer cells

Sun

Liu²,

Wang³

et al. 1994

Oncol Rep

View full text Add to dashboard Cite

Abstract. Spermidine/spermine N 1 -acetyltransferase (SSAT) is a key enzyme of polyamine catabolism. In a previous study, we constructed a recombinant adenovirus, Ad-SSAT, which can express human SSAT. In the present study, we investigated the effect of Ad-SSAT on the growth and cell cycle of colorectal cancer cells. We found that Ad-SSAT increased the expression of SSAT and inhibited the growth of HT-29 and LoVo cells. The growth inhibition was caused by cell cycle arrest in the S phase. Furthermore, Ad-SSAT was shown to suppress the expression of cyclin A and nuclear factor E2F-1 in HT-29 and LoVo cells. The inhibitory effect of Ad-SSAT on cyclin A promoter activity was also observed in a reporter gene assay. Our results suggest that the expression of SSAT mediated by Ad-SSAT infection inhibits the growth of colorectal cancer cells and induces cell cycle arrest at the S phase, through a mechanism involving the suppression of cyclin A and E2F-1 expression.

show abstract

Polyamines in colorectal cancer. Evaluation of polyamine concentrations in the colon tissue, serum, and urine of 50 patients with colorectal cancer

Cited by 87 publications

References 25 publications

The clinical usefulness of urinary N1,N12-diacetylspermine (DiAcSpm) levels as a tumor marker in patients with colorectal cancer

The clinical usefulness of urinary N1,N12-diacetylspermine (DiAcSpm) levels as a tumor marker in patients with colorectal cancer

Polyamines in human health

Adenovirus-mediated expression of spermidine/spermine N1-acetyltransferase gene induces S-phase arrest in human colorectal cancer cells

Contact Info

Product

Resources

About