Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide

Papadakis, Anastasia K.; Roubelakis‐Angelakis, Kalliopi A.

doi:10.1007/s00425-004-1400-9

Cited by 120 publications

(97 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results are supported by the work of Ren et al [26] in Arabidopsis thaliana where they observed that the root length reduced by the jasmonic acid alone treatment, was restored on supplementation of jasmonic acid with epibrassinolide. The results of our experiments proved the hypothesis Modulation of jasmonic acid and polyamines by 24- [27]. The amino groups present in PAs undergo reversible protonation.…”

Section: Discussionsupporting

confidence: 81%

Modulation of jasmonic acid and polyamines by 24- epibrassinolide in Brassica juncea L. under copper stress

Kaur¹,

Bhardwaj²,

Thukral³

2016

IOSR

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 81%

Modulation of jasmonic acid and polyamines by 24- epibrassinolide in Brassica juncea L. under copper stress

Kaur¹,

Bhardwaj²,

Thukral³

2016

IOSR

View full text Add to dashboard Cite

“…Arabidopsis Actin8 as was used as an internal control to normalize gene expression level. al., 2003), and Spd catabolism by PAO results in hydrogen peroxide production, which at low concentration functions as a signaling molecule stimulating the induction of the antioxidant machinery (Papadakis and Roubelakis-Angelakis, 2005;Moschou et al, 2008a). Our documentation of the activation of antioxidant genes in 10A06-overexpressing as well as in SPDS2-overexpressing Arabidopsis plants infected with H. schachtii provides support for ROS scavenging as a likely mechanism of 10A06 effector function.…”

Section: Discussionmentioning

confidence: 53%

Arabidopsis Spermidine Synthase Is Targeted by an Effector Protein of the Cyst Nematode Heterodera schachtii

et al. 2009

View full text Add to dashboard Cite

Cyst nematodes are sedentary plant parasites that cause dramatic cellular changes in the plant root to form feeding cells, socalled syncytia. 10A06 is a cyst nematode secretory protein that is most likely secreted as an effector into the developing syncytia during early plant parasitism. A homolog of the uncharacterized soybean cyst nematode (Heterodera glycines), 10A06 gene was cloned from the sugar beet cyst nematode (Heterodera schachtii), which is able to infect Arabidopsis (Arabidopsis thaliana). Constitutive expression of 10A06 in Arabidopsis affected plant morphology and increased susceptibility to H. schachtii as well as to other plant pathogens. Using yeast two-hybrid assays, we identified Spermidine Synthase2 (SPDS2), a key enzyme involved in polyamine biosynthesis, as a specific 10A06 interactor. In support of this protein-protein interaction, transgenic plants expressing 10A06 exhibited elevated SPDS2 mRNA abundance, significantly higher spermidine content, and increased polyamine oxidase (PAO) activity. Furthermore, the SPDS2 promoter was strongly activated in the nematode-induced syncytia, and transgenic plants overexpressing SPDS2 showed enhanced plant susceptibility to H. schachtii. In addition, in planta expression of 10A06 or SPDS2 increased mRNA abundance of a set of antioxidant genes upon nematode infection. These data lend strong support to a model in which the cyst nematode effector 10A06 exerts its function through the interaction with SPDS2, thereby increasing spermidine content and subsequently PAO activity. Increasing PAO activity results in stimulating the induction of the cellular antioxidant machinery in syncytia. Furthermore, we observed an apparent disruption of salicylic acid defense signaling as a function of 10A06. Most likely, increased antioxidant protection and interruption of salicylic acid signaling are key aspects of 10A06 function in addition to other physiological and morphological changes caused by altered polyamines, which are potent plant signaling molecules.Sedentary plant-parasitic nematodes are obligate biotrophs that meet their nutritional requirements solely from modified, but living, root cells of their host plants. The most economically important plantparasitic nematodes are the sedentary endoparasitic root-knot nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera and Globodera spp.). The soybean cyst nematode (Heterodera glycines) and the closely related sugar beet cyst nematode (Heterodera schachtii) are of particular importance in U.S. agriculture.Cyst nematode infection involves the penetration of second-stage juveniles (J2) into host roots followed by intracellular migration to the vasculature, where the nematodes initiate specialized feeding structures called syncytia. Feeding site initiation and formation are characterized by complex morphological and physiological changes of the parasitized plant root cells. This process includes endoreduplication, cell wall modification and dissolution leading to cell fusion, disappearance of large vacuoles, inc...

show abstract

“…Spermidine has the same precursor as aminocyclopropane, an important source of ethylene that can induce senescence prematurely (Imai et al, 2004). However, the accumulation of the polyamines might not be an ARC per se but may rather reflect a protection mechanism against ROS generation and programmed cell death (Papadakis and Roubelakis-Angelakis, 2005). Moreover, polyamines such as cytokinins are well known for their senescence-retarding effect.…”

Section: The Old5 Mutation Results In a Metabolite Profile Characterimentioning

confidence: 99%

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

et al. 2008

View full text Add to dashboard Cite

Leaf senescence in Arabidopsis thaliana is a strict, genetically controlled nutrient recovery program, which typically progresses in an age-dependent manner. Leaves of the Arabidopsis onset of leaf death5 (old5) mutant exhibit early developmental senescence. Here, we show that OLD5 encodes quinolinate synthase (QS), a key enzyme in the de novo synthesis of NAD. The Arabidopsis QS was previously shown to carry a Cys desulfurase domain that stimulates reconstitution of the oxygen-sensitive Fe-S cluster that is required for QS activity. The old5 lesion in this enzyme does not affect QS activity but it decreases its Cys desulfurase activity and thereby the long-term catalytic competence of the enzyme. The old5 mutation causes increased NAD steady state levels that coincide with increased activity of enzymes in the NAD salvage pathway. NAD plays a key role in cellular redox reactions, including those of the tricarboxylic acid cycle. Broad-range metabolite profiling of the old5 mutant revealed that it contains higher levels of tricarboxylic acid cycle intermediates and nitrogen-containing amino acids. The mutant displays a higher respiration rate concomitant with increased expression of oxidative stress markers. We postulate that the alteration in the oxidative state is integrated into the plant developmental program, causing early ageing of the mutant.

show abstract

Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide

Cited by 120 publications

References 54 publications

Modulation of jasmonic acid and polyamines by 24- epibrassinolide in Brassica juncea L. under copper stress

Modulation of jasmonic acid and polyamines by 24- epibrassinolide in Brassica juncea L. under copper stress

Arabidopsis Spermidine Synthase Is Targeted by an Effector Protein of the Cyst Nematode Heterodera schachtii

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

Contact Info

Product

Resources

About